LSU VETERINARY COBRE: MOLECULAR IMMUNOPATHOLOGY CORE

路易斯安那州立大学兽医 COBRE：分子免疫病理学核心

• 批准号: 8359776
• 负责人: Konstantin G Kousoulas
• 金额: $ 52.23万
• 依托单位: LOUISIANA STATE UNIV A&M COL BATON ROUGE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8359776
• 关键词: Animal Model; Antibody Formation; Biological Assay; Biometry; Cell Separation; Cells; Centers of Research Excellence; Clinical Pathology; Communicable Diseases; Confocal Microscopy; Consultations; Custom; DNA Library; DNA Sequence; Diagnosis; Ensure; Funding; Future; Grant; Histocytochemistry; Immune; In Situ Hybridization; In Vitro; Infectious Diseases Research; Laboratories; Lasers; Louisiana; Lymphokines; Microscopy; Molecular; Molecular Biology; National Center for Research Resources; Nature; Pathologist; Pathology; Principal Investigator; Production; Recombinant DNA; Research; Research Infrastructure; Research Personnel; Resources; Services; Source; Technology; Technology Transfer; Time; Tissues; Training; Transmission Electron Microscopy; United States National Institutes of Health; Universities; Viral; Work; base; cDNA Library Construction; cost; immunopathology; in vivo; molecular pathology; new technology; nonhuman primate; pathogen; protein profiling; synthetic polymer Bioplex

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Louisiana State University COBRE Molecular Immunopathology (MIP) Core is a consortium arrangement of five existing Centralized Facilities and Service Units of the LSU-SVM working together to provide state-of-the art service, as well as to develop new technologies to detect, diagnose and analyze the effect of infectious disease pathogens in vitro and in vivo. Dr. Xavier Alvarez (TNPRC) and Dr. David Scollard (NHDP) will participate in the MIP Core. Their participation ensures technology transfer of state-of-the art confocal and molecular pathological technologies already implemented for non-human primate and other animal models to researchers at LSU-SVM and the COBRE-CEIDR. The MIP Consortium is composed of the following existing SVM Centralized Facilities: 1) BIOMMED concentrates on molecular biology and currently provides advanced recombinant DNA and cDNA library constructions, real-time PCR, DNA sequencing, BioPlex lymphokine profiling, protein production and purification, custom antibody production etc. 2) The Centralized Pathology Service Laboratory currently provides routine anatomical, clinical and molecular pathology, the later including immuno-histochemical approaches to detect pathogens and specialized cells. A specialized Unit wihin this Service Laboratory has been formed under the direction of Dr. Nobuko Wakamatsu (Board Certified Pathologist). This unit will be an intimate part of the MIP Core and will serve as liason to other pathology services. 3) the FACS Facility provides advanced immunological assays for lymphokine profiling, cell sorting, etc. 4) The Microscopy Center provides, transmission and electron microscopy, confocal microscopy and laser capture microscopy. New assays that will be developed by the MIP Core include the use of in situ hybridization to detect, viral , rickettsial and bacterial pathogens, tissue immunoprofiling to determine via confocal and immuno-histochemistry the nature of infiltrating immune cells within diseased tissues, etc. 5) The Biostatistics Service Unit provides statistical consultation and training to LSU-based researchers. It is expected that MIP will be a strong supporting Core of the LSU-SVM-based COBRE-CEIDR in future years receiving support from LSU-SVM, as well as other state and federal sources.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 路易斯安那州立大学COBRE分子免疫病理学（MIP）核心是LSU-SVM的五个现有集中设施和服务单位的联盟安排，共同提供最先进的服务，以及开发新技术来检测，诊断和分析体外和体内传染病病原体的影响。泽维尔·阿尔瓦雷斯博士（TNPRC）和大卫·斯科拉德博士（NHDP）将参加MIP核心。他们的参与确保了已经用于非人类灵长类动物和其他动物模型的最先进的共聚焦和分子病理学技术向LSU-SVM和COBRE-CEIDR的研究人员的技术转让。MIP联合体由以下现有的SVM集中设施组成：1）BIOMMED专注于分子生物学，目前提供先进的重组DNA和cDNA文库构建，实时PCR，DNA测序，生物学淋巴因子分析，蛋白质生产和纯化，定制抗体生产等。2）中央病理服务实验室目前提供常规解剖，临床和分子病理学，后者包括检测病原体和特化细胞的免疫组织化学方法。在Nobuko Wakamatsu博士（委员会认证病理学家）的指导下，在该服务实验室中成立了一个专门单位。该单位将是MIP核心的一个重要组成部分，并将作为其他病理服务的联络人。3)流式细胞仪设备提供先进的免疫学分析，用于淋巴因子分析、细胞分选等。4）显微镜中心提供透射和电子显微镜、共聚焦显微镜和激光捕获显微镜。MIP Core将开发的新检测方法包括使用原位杂交检测病毒、立克次体和细菌病原体，通过共聚焦和免疫组织化学确定病变组织内浸润免疫细胞的性质的组织免疫分析等。预计MIP将在未来几年成为基于LSU-SVM的COBRE-CEIDR的强有力的支持核心，得到LSU-SVM以及其他州和联邦来源的支持。