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Induction of retinal development by the peripodial epithelium in Drosophila

果蝇足周上皮诱导视网膜发育

基本信息

批准号：
10093048
负责人：
Justin P Kumar
金额：
$ 45.4万
依托单位：
TRUSTEES OF INDIANA UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-02-01 至 2024-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10093048
关键词：
Address Adult Amphibia Animals Attention BMP4 Blastopores Brain Cells Columnar Epithelium Communication Complex Crystalline Lens Cuboidal Cell Cues Data Development Diffuse Drosophila eye Drosophila genus Drosophila melanogaster Ectoderm Elements Embryonic Development Endoderm Ensure Epidermis Epithelial Event Excision Eye Eye Development Future Genetic Genetic Transcription Goals Growth Head Heart Individual Knowledge Lead Ligands Lip structure Literature Mammals Maxilla Mediating Mesoderm Methods Modeling Modernization Molecular Morphogenesis Mutation Newts Notch Signaling Pathway Organism Pathway interactions Pattern Population Positioning Attribute Process Production Public Health Publishing Regulator Genes Retina Retinal Diseases Role Signal Induction Signal Pathway Signal Transduction Signaling Molecule Somites Squamous Cell Structure System Testing Thinness Tissues Transforming Growth Factor beta Transplantation Vertebrates Visual Fields Visual system structure cell type compound eye design eye formation fly insight interest lens neglect neural plate novel tool transcription factor

项目摘要

Project Summary: Induction, the process by which one tissue signals to and influences the development of another, is a central feature of metazoan development. Some of the most famous and best studied examples of inductive interactions include the communication that takes place between the ectoderm, mesoderm, and endoderm during early embryonic development. Other examples include the signaling that leads to proper neural plate, somite, and brain development. Relevant to this proposal are the inductive cues that are sent by the vertebrate lens to ensure proper specification, positioning, and patterning of the adjacent retina. Mutations that either disrupt transcriptional networks within and signaling emanating from the lens lead to catastrophic retinal disorders. As such, there is intense interest in identifying and understanding the mechanisms underlying the induction of retinal development by the adjoining lens. This application is focused on using the eye- antennal disc of the fruit fly, Drosophila melanogaster, as an experimental system for studying inductive events during eye formation. The eye-antennal disc is a sac-like structure that contains three different tissues. The retina develops from a columnar epithelium called the disc proper. Overlying the disc proper is a sheet of squamous cells called the peripodial epithelium. These two layers are stitched together along their edges by a strip of cuboidal cells called the margin (which is itself derived from the peripodial epithelium). As such, the eye-antennal disc resembles a closed pillowcase. Evidence from the published literature indicates that signaling from the peripodial epithelium is important for inducing fate specification, growth, patterning, and cell fate choices within the retina. While the vertebrate lens and fly peripodial epithelium are non-homologous structures it appears that both tissues make use of common regulatory modules to induce developmental changes in the retina. For example, recent studies have shown that Pax6 and BMP4/TGFβ signaling are both required in the lens and peripodial epithelium for retinal development. In this proposal we will address a number of exciting questions that go to the heart understanding how development of the retina is induced by neighboring tissues. Using modern molecular, cellular, and genetic methods we will develop a state-of-the-art contemporary perspective on how the peripodial epithelium influences the development of the eye and directly contributes to formation of the head. As part of these studies we will pursue the identification of transcription factors and signaling pathways that are important in the peripodial epithelium for retinal development. These gene regulatory networks will be relevant to understanding how the vertebrate lens communicates to the adjacent retina. We will test the specific hypotheses that Pax6 and the So-Eya complex regulate the production of ligands for the TGFβ and Notch signaling pathways. From the aims presented here we will acquire new insights into the mechanisms by which transcriptional networks and signaling pathways are integrated to control inductive events during retinal specification and patterning.

项目概述：诱导，一个组织向…发出信号并影响…发育的过程