权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Induction of retinal development by the peripodial epithelium in Drosophila

果蝇足周上皮诱导视网膜发育

基本信息

批准号：
10570224
负责人：
Justin P Kumar
金额：
$ 34.49万
依托单位：
TRUSTEES OF INDIANA UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-02-01 至 2025-01-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10570224
关键词：
Adult Amphibia Animals Attention BMP4 Blastopores Brain Cells Columnar Epithelium Communication Complex Cuboidal Cell Cues Data Development Diffuse Drosophila eye Drosophila genus Drosophila melanogaster Ectoderm Elements Embryonic Development Endoderm Ensure Epidermis Epithelium Event Excision Eye Eye Development Future Genetic Genetic Transcription Goals Growth Head Heart Individual Knowledge Lead Ligands Lip structure Literature Mammals Maxilla Mediating Mesoderm Methods Modeling Modernization Molecular Morphogenesis Mutation Newts Notch Signaling Pathway Organism Pathway interactions Pattern Population Positioning Attribute Process Production Public Health Publishing Retina Retinal Diseases Role Signal Induction Signal Pathway Signal Transduction Signaling Molecule Somites Specific qualifier value Squamous Cell Structure System Testing Thinness Tissues Transforming Growth Factor beta Transplantation Vertebrates Visual Fields Visual System cell type compound eye design eye formation fly gastrulation gene regulatory network insight interest lens neglect neural plate novel tool transcription factor

项目摘要

Project Summary: Induction, the process by which one tissue signals to and influences the development of another, is a central feature of metazoan development. Some of the most famous and best studied examples of inductive interactions include the communication that takes place between the ectoderm, mesoderm, and endoderm during early embryonic development. Other examples include the signaling that leads to proper neural plate, somite, and brain development. Relevant to this proposal are the inductive cues that are sent by the vertebrate lens to ensure proper specification, positioning, and patterning of the adjacent retina. Mutations that either disrupt transcriptional networks within and signaling emanating from the lens lead to catastrophic retinal disorders. As such, there is intense interest in identifying and understanding the mechanisms underlying the induction of retinal development by the adjoining lens. This application is focused on using the eye- antennal disc of the fruit fly, Drosophila melanogaster, as an experimental system for studying inductive events during eye formation. The eye-antennal disc is a sac-like structure that contains three different tissues. The retina develops from a columnar epithelium called the disc proper. Overlying the disc proper is a sheet of squamous cells called the peripodial epithelium. These two layers are stitched together along their edges by a strip of cuboidal cells called the margin (which is itself derived from the peripodial epithelium). As such, the eye-antennal disc resembles a closed pillowcase. Evidence from the published literature indicates that signaling from the peripodial epithelium is important for inducing fate specification, growth, patterning, and cell fate choices within the retina. While the vertebrate lens and fly peripodial epithelium are non-homologous structures it appears that both tissues make use of common regulatory modules to induce developmental changes in the retina. For example, recent studies have shown that Pax6 and BMP4/TGFβ signaling are both required in the lens and peripodial epithelium for retinal development. In this proposal we will address a number of exciting questions that go to the heart understanding how development of the retina is induced by neighboring tissues. Using modern molecular, cellular, and genetic methods we will develop a state-of-the-art contemporary perspective on how the peripodial epithelium influences the development of the eye and directly contributes to formation of the head. As part of these studies we will pursue the identification of transcription factors and signaling pathways that are important in the peripodial epithelium for retinal development. These gene regulatory networks will be relevant to understanding how the vertebrate lens communicates to the adjacent retina. We will test the specific hypotheses that Pax6 and the So-Eya complex regulate the production of ligands for the TGFβ and Notch signaling pathways. From the aims presented here we will acquire new insights into the mechanisms by which transcriptional networks and signaling pathways are integrated to control inductive events during retinal specification and patterning.

项目摘要：诱导，一个组织向细胞发出信号并影响细胞发育的过程另一个是后生动物发育的核心特征。一些最著名和研究最多的例子诱导性相互作用包括发生在外胚层、中胚层和早期胚胎发育过程中的内胚层。其他例子包括导致正确的信号神经板、体节和大脑发育。与该提案相关的是由以下人员发送的归纳线索脊椎动物晶状体，以确保相邻视网膜的正确规格、定位和图案。突变破坏晶状体内部的转录网络和从晶状体发出的信号会导致灾难性的后果视网膜疾病。因此，人们对识别和理解其背后的机制产生了浓厚的兴趣。通过相邻晶状体诱导视网膜发育。该应用程序的重点是使用眼睛果蝇的触角盘，作为研究诱导事件的实验系统在眼睛形成过程中。眼触角盘是一个囊状结构，包含三种不同的组织。这视网膜由称为椎间盘的柱状上皮发育而来。覆盖在光盘上的是一张鳞状细胞称为足周上皮。这两层通过边缘缝合在一起称为边缘的立方形细胞带（其本身源自足周上皮）。因此，眼触角盘类似于封闭的枕套。已发表文献的证据表明来自足周上皮的信号传导对于诱导命运规范、生长、模式化和细胞发育非常重要。视网膜内的命运选择。虽然脊椎动物的晶状体和果蝇的足周上皮是非同源的结构似乎两种组织都利用共同的调节模块来诱导发育视网膜的变化。例如，最近的研究表明 Pax6 和 BMP4/TGFβ 信号传导均视网膜发育所需的晶状体和足周上皮。在本提案中，我们将解决许多令人兴奋的问题深入人心，了解视网膜的发育是如何引起的邻近组织。使用现代分子、细胞和遗传方法，我们将开发最先进的关于足周上皮如何影响眼睛发育并直接影响的当代观点有助于头部的形成。作为这些研究的一部分，我们将寻求转录的鉴定足周上皮对视网膜发育很重要的因子和信号通路。这些基因调控网络将有助于理解脊椎动物晶状体如何与邻近的视网膜。我们将测试 Pax6 和 So-Eya 复合体调节生产的具体假设 TGFβ 和 Notch 信号通路的配体。根据这里提出的目标，我们将获得新的深入了解转录网络和信号通路整合的机制控制视网膜规范和图案化过程中的感应事件。

项目成果

期刊论文数量（8）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Creating the eye-antennal disc by fission.

通过裂变形成眼触角盘。

DOI：
10.1093/genetics/iyac169
发表时间：
2023
期刊：
Genetics
影响因子：
3.3
作者：
Kumar,JustinP
通讯作者：
Kumar,JustinP

A CUT&RUN protocol to determine patterns of epigenetic marks in imaginal discs of Drosophila.

DOI：
10.1016/j.xpro.2022.101878
发表时间：
2023-03-17
期刊：
STAR PROTOCOLS
影响因子：
0
作者：
Weasner, Brandon P.;Brown, Haley E.;Policastro, Robert;Weasner, Bonnie M.;Kumar, Justin P.
通讯作者：
Kumar, Justin P.

Patterning of the Drosophila retina by the morphogenetic furrow.