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Induction of retinal development by the peripodial epithelium in Drosophila

果蝇足周上皮诱导视网膜发育

基本信息

批准号：
10570224
负责人：
Justin P Kumar
金额：
$ 34.49万
依托单位：
TRUSTEES OF INDIANA UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-02-01 至 2025-01-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10570224
关键词：
Adult Amphibia Animals Attention BMP4 Blastopores Brain Cells Columnar Epithelium Communication Complex Cuboidal Cell Cues Data Development Diffuse Drosophila eye Drosophila genus Drosophila melanogaster Ectoderm Elements Embryonic Development Endoderm Ensure Epidermis Epithelium Event Excision Eye Eye Development Future Genetic Genetic Transcription Goals Growth Head Heart Individual Knowledge Lead Ligands Lip structure Literature Mammals Maxilla Mediating Mesoderm Methods Modeling Modernization Molecular Morphogenesis Mutation Newts Notch Signaling Pathway Organism Pathway interactions Pattern Population Positioning Attribute Process Production Public Health Publishing Retina Retinal Diseases Role Signal Induction Signal Pathway Signal Transduction Signaling Molecule Somites Specific qualifier value Squamous Cell Structure System Testing Thinness Tissues Transforming Growth Factor beta Transplantation Vertebrates Visual Fields Visual System cell type compound eye design eye formation fly gastrulation gene regulatory network insight interest lens neglect neural plate novel tool transcription factor

项目摘要

Project Summary: Induction, the process by which one tissue signals to and influences the development of another, is a central feature of metazoan development. Some of the most famous and best studied examples of inductive interactions include the communication that takes place between the ectoderm, mesoderm, and endoderm during early embryonic development. Other examples include the signaling that leads to proper neural plate, somite, and brain development. Relevant to this proposal are the inductive cues that are sent by the vertebrate lens to ensure proper specification, positioning, and patterning of the adjacent retina. Mutations that either disrupt transcriptional networks within and signaling emanating from the lens lead to catastrophic retinal disorders. As such, there is intense interest in identifying and understanding the mechanisms underlying the induction of retinal development by the adjoining lens. This application is focused on using the eye- antennal disc of the fruit fly, Drosophila melanogaster, as an experimental system for studying inductive events during eye formation. The eye-antennal disc is a sac-like structure that contains three different tissues. The retina develops from a columnar epithelium called the disc proper. Overlying the disc proper is a sheet of squamous cells called the peripodial epithelium. These two layers are stitched together along their edges by a strip of cuboidal cells called the margin (which is itself derived from the peripodial epithelium). As such, the eye-antennal disc resembles a closed pillowcase. Evidence from the published literature indicates that signaling from the peripodial epithelium is important for inducing fate specification, growth, patterning, and cell fate choices within the retina. While the vertebrate lens and fly peripodial epithelium are non-homologous structures it appears that both tissues make use of common regulatory modules to induce developmental changes in the retina. For example, recent studies have shown that Pax6 and BMP4/TGFβ signaling are both required in the lens and peripodial epithelium for retinal development. In this proposal we will address a number of exciting questions that go to the heart understanding how development of the retina is induced by neighboring tissues. Using modern molecular, cellular, and genetic methods we will develop a state-of-the-art contemporary perspective on how the peripodial epithelium influences the development of the eye and directly contributes to formation of the head. As part of these studies we will pursue the identification of transcription factors and signaling pathways that are important in the peripodial epithelium for retinal development. These gene regulatory networks will be relevant to understanding how the vertebrate lens communicates to the adjacent retina. We will test the specific hypotheses that Pax6 and the So-Eya complex regulate the production of ligands for the TGFβ and Notch signaling pathways. From the aims presented here we will acquire new insights into the mechanisms by which transcriptional networks and signaling pathways are integrated to control inductive events during retinal specification and patterning.

项目摘要：诱导，一个组织发出信号并影响其发展的过程另一个是后生动物发展的中心特征。一些最著名和研究最深入的例子诱导相互作用包括发生在外胚层、中胚层和胚胎发育早期的内胚层。其他例子包括导致适当的信号发送神经板、体节和大脑发育。与这项提议相关的是由脊椎动物的晶状体，以确保相邻视网膜的适当规格、位置和图案。突变要么扰乱晶状体内部的转录网络，要么从晶状体发出信号导致灾难性的视网膜疾病。因此，人们对确定和了解潜在的机制有着浓厚的兴趣由邻近的晶状体引起的视网膜发育。这个应用程序的重点是使用眼睛- 果蝇触角盘作为研究诱导事件的实验系统在眼睛形成过程中。眼球触角盘是一个囊状结构，包含三个不同的组织。这个视网膜由一种叫做视盘本身的柱状上皮发育而来。覆盖在光盘上的是一张鳞状细胞称为足周上皮。这两个层是通过一个长条状的立方细胞，称为边缘(其本身来源于足周上皮)。因此，眼睛触角盘就像一个封闭的枕套。已出版文献中的证据表明来自足周上皮的信号对于诱导命运规范、生长、图案化和细胞是重要的。视网膜内的命运选择。而脊椎动物的晶状体和苍蝇的牙周上皮是不同源的结构似乎这两个组织都利用共同的调节模块来诱导发育视网膜的变化。例如，最近的研究表明，Pax6和BMP4/转化生长因子β信号都是视网膜发育所需的晶状体和足周上皮细胞。在这项提案中，我们将解决一些令人兴奋的问题进入心脏，了解视网膜是如何由邻近的组织。使用现代分子、细胞和遗传方法，我们将开发出一种最先进的关于足周上皮如何直接影响眼睛发育的当代观点有助于头部的形成。作为这些研究的一部分，我们将继续研究转录的鉴定在视网膜发育过程中，在视网膜周围上皮中起重要作用的因素和信号通路。这些基因调控网络将与理解脊椎动物晶状体如何与相邻的视网膜。我们将检验Pax6和So-Eya复合体调节生产的具体假设转化生长因子β和Notch信号通路的配体。从这里提出的目标中，我们将获得新的对转录网络和信号通路整合机制的洞察控制视网膜规范和图案化过程中的感应事件。

项目成果

期刊论文数量（8）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Creating the eye-antennal disc by fission.

通过裂变形成眼触角盘。

DOI：
10.1093/genetics/iyac169
发表时间：
2023
期刊：
Genetics
影响因子：
3.3
作者：
Kumar,JustinP
通讯作者：
Kumar,JustinP

A CUT&RUN protocol to determine patterns of epigenetic marks in imaginal discs of Drosophila.

DOI：
10.1016/j.xpro.2022.101878
发表时间：
2023-03-17
期刊：
STAR PROTOCOLS
影响因子：
0
作者：
Weasner, Brandon P.;Brown, Haley E.;Policastro, Robert;Weasner, Bonnie M.;Kumar, Justin P.
通讯作者：
Kumar, Justin P.

Patterning of the Drosophila retina by the morphogenetic furrow.