Induction of retinal development by the peripodial epithelium in Drosophila
果蝇足周上皮诱导视网膜发育
基本信息
- 批准号:10570224
- 负责人:
- 金额:$ 34.49万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2020
- 资助国家:美国
- 起止时间:2020-02-01 至 2025-01-31
- 项目状态:未结题
- 来源:
- 关键词:AdultAmphibiaAnimalsAttentionBMP4BlastoporesBrainCellsColumnar EpitheliumCommunicationComplexCuboidal CellCuesDataDevelopmentDiffuseDrosophila eyeDrosophila genusDrosophila melanogasterEctodermElementsEmbryonic DevelopmentEndodermEnsureEpidermisEpitheliumEventExcisionEyeEye DevelopmentFutureGeneticGenetic TranscriptionGoalsGrowthHeadHeartIndividualKnowledgeLeadLigandsLip structureLiteratureMammalsMaxillaMediatingMesodermMethodsModelingModernizationMolecularMorphogenesisMutationNewtsNotch Signaling PathwayOrganismPathway interactionsPatternPopulationPositioning AttributeProcessProductionPublic HealthPublishingRetinaRetinal DiseasesRoleSignal InductionSignal PathwaySignal TransductionSignaling MoleculeSomitesSpecific qualifier valueSquamous CellStructureSystemTestingThinnessTissuesTransforming Growth Factor betaTransplantationVertebratesVisual FieldsVisual Systemcell typecompound eyedesigneye formationflygastrulationgene regulatory networkinsightinterestlensneglectneural platenoveltooltranscription factor
项目摘要
Project Summary: Induction, the process by which one tissue signals to and influences the development of
another, is a central feature of metazoan development. Some of the most famous and best studied examples
of inductive interactions include the communication that takes place between the ectoderm, mesoderm, and
endoderm during early embryonic development. Other examples include the signaling that leads to proper
neural plate, somite, and brain development. Relevant to this proposal are the inductive cues that are sent by
the vertebrate lens to ensure proper specification, positioning, and patterning of the adjacent retina. Mutations
that either disrupt transcriptional networks within and signaling emanating from the lens lead to catastrophic
retinal disorders. As such, there is intense interest in identifying and understanding the mechanisms underlying
the induction of retinal development by the adjoining lens. This application is focused on using the eye-
antennal disc of the fruit fly, Drosophila melanogaster, as an experimental system for studying inductive events
during eye formation. The eye-antennal disc is a sac-like structure that contains three different tissues. The
retina develops from a columnar epithelium called the disc proper. Overlying the disc proper is a sheet of
squamous cells called the peripodial epithelium. These two layers are stitched together along their edges by a
strip of cuboidal cells called the margin (which is itself derived from the peripodial epithelium). As such, the
eye-antennal disc resembles a closed pillowcase. Evidence from the published literature indicates that
signaling from the peripodial epithelium is important for inducing fate specification, growth, patterning, and cell
fate choices within the retina. While the vertebrate lens and fly peripodial epithelium are non-homologous
structures it appears that both tissues make use of common regulatory modules to induce developmental
changes in the retina. For example, recent studies have shown that Pax6 and BMP4/TGFβ signaling are both
required in the lens and peripodial epithelium for retinal development. In this proposal we will address a
number of exciting questions that go to the heart understanding how development of the retina is induced by
neighboring tissues. Using modern molecular, cellular, and genetic methods we will develop a state-of-the-art
contemporary perspective on how the peripodial epithelium influences the development of the eye and directly
contributes to formation of the head. As part of these studies we will pursue the identification of transcription
factors and signaling pathways that are important in the peripodial epithelium for retinal development. These
gene regulatory networks will be relevant to understanding how the vertebrate lens communicates to the
adjacent retina. We will test the specific hypotheses that Pax6 and the So-Eya complex regulate the production
of ligands for the TGFβ and Notch signaling pathways. From the aims presented here we will acquire new
insights into the mechanisms by which transcriptional networks and signaling pathways are integrated to
control inductive events during retinal specification and patterning.
项目摘要:诱导,一个组织向细胞发出信号并影响细胞发育的过程
另一个是后生动物发育的核心特征。一些最著名和研究最多的例子
诱导性相互作用包括发生在外胚层、中胚层和
早期胚胎发育过程中的内胚层。其他例子包括导致正确的信号
神经板、体节和大脑发育。与该提案相关的是由以下人员发送的归纳线索
脊椎动物晶状体,以确保相邻视网膜的正确规格、定位和图案。突变
破坏晶状体内部的转录网络和从晶状体发出的信号会导致灾难性的后果
视网膜疾病。因此,人们对识别和理解其背后的机制产生了浓厚的兴趣。
通过相邻晶状体诱导视网膜发育。该应用程序的重点是使用眼睛
果蝇的触角盘,作为研究诱导事件的实验系统
在眼睛形成过程中。眼触角盘是一个囊状结构,包含三种不同的组织。这
视网膜由称为椎间盘的柱状上皮发育而来。覆盖在光盘上的是一张
鳞状细胞称为足周上皮。这两层通过边缘缝合在一起
称为边缘的立方形细胞带(其本身源自足周上皮)。因此,
眼触角盘类似于封闭的枕套。已发表文献的证据表明
来自足周上皮的信号传导对于诱导命运规范、生长、模式化和细胞发育非常重要。
视网膜内的命运选择。虽然脊椎动物的晶状体和果蝇的足周上皮是非同源的
结构似乎两种组织都利用共同的调节模块来诱导发育
视网膜的变化。例如,最近的研究表明 Pax6 和 BMP4/TGFβ 信号传导均
视网膜发育所需的晶状体和足周上皮。在本提案中,我们将解决
许多令人兴奋的问题深入人心,了解视网膜的发育是如何引起的
邻近组织。使用现代分子、细胞和遗传方法,我们将开发最先进的
关于足周上皮如何影响眼睛发育并直接影响的当代观点
有助于头部的形成。作为这些研究的一部分,我们将寻求转录的鉴定
足周上皮对视网膜发育很重要的因子和信号通路。这些
基因调控网络将有助于理解脊椎动物晶状体如何与
邻近的视网膜。我们将测试 Pax6 和 So-Eya 复合体调节生产的具体假设
TGFβ 和 Notch 信号通路的配体。根据这里提出的目标,我们将获得新的
深入了解转录网络和信号通路整合的机制
控制视网膜规范和图案化过程中的感应事件。
项目成果
期刊论文数量(8)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Creating the eye-antennal disc by fission.
通过裂变形成眼触角盘。
- DOI:10.1093/genetics/iyac169
- 发表时间:2023
- 期刊:
- 影响因子:3.3
- 作者:Kumar,JustinP
- 通讯作者:Kumar,JustinP
A CUT&RUN protocol to determine patterns of epigenetic marks in imaginal discs of Drosophila.
- DOI:10.1016/j.xpro.2022.101878
- 发表时间:2023-03-17
- 期刊:
- 影响因子:0
- 作者:Weasner, Brandon P.;Brown, Haley E.;Policastro, Robert;Weasner, Bonnie M.;Kumar, Justin P.
- 通讯作者:Kumar, Justin P.
Patterning of the Drosophila retina by the morphogenetic furrow.
- DOI:10.3389/fcell.2023.1151348
- 发表时间:2023
- 期刊:
- 影响因子:5.5
- 作者:
- 通讯作者:
Development of the ocellar visual system in Drosophila melanogaster.
果蝇眼视觉系统的发育。
- DOI:10.1111/febs.16468
- 发表时间:2022-12
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Polycomb safeguards imaginal disc specification through control of the Vestigial-Scalloped complex.
Polycomb 通过控制残留-扇形复合体来保护成虫盘的规格。
- DOI:10.1101/2023.04.11.536444
- 发表时间:2023
- 期刊:
- 影响因子:0
- 作者:Brown,HaleyE;Weasner,BrandonP;Weasner,BonnieM;Kumar,JustinP
- 通讯作者:Kumar,JustinP
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{{ truncateString('Justin P Kumar', 18)}}的其他基金
Induction of retinal development by the peripodial epithelium in Drosophila
果蝇足周上皮诱导视网膜发育
- 批准号:
10190594 - 财政年份:2020
- 资助金额:
$ 34.49万 - 项目类别:
Induction of retinal development by the peripodial epithelium in Drosophila
果蝇足周上皮诱导视网膜发育
- 批准号:
10093048 - 财政年份:2020
- 资助金额:
$ 34.49万 - 项目类别:
Induction of retinal development by the peripodial epithelium in Drosophila
果蝇足周上皮诱导视网膜发育
- 批准号:
10356811 - 财政年份:2020
- 资助金额:
$ 34.49万 - 项目类别:
Function of the Six and Eya Genes in Retinal Development
Six 和 Eya 基因在视网膜发育中的功能
- 批准号:
8694777 - 财政年份:2003
- 资助金额:
$ 34.49万 - 项目类别:
Function of the Six and Eya Genes in Retinal Development
Six 和 Eya 基因在视网膜发育中的功能
- 批准号:
7110936 - 财政年份:2003
- 资助金额:
$ 34.49万 - 项目类别:
Repression of Non-Ocular Fates by the SIX and EYA Genes During Specification and Patterning of the Retina
视网膜规范和图案化过程中 SIX 和 EYA 基因对非眼命运的抑制
- 批准号:
9135417 - 财政年份:2003
- 资助金额:
$ 34.49万 - 项目类别:
Function of the Six and Eya Genes in Retinal Development
Six 和 Eya 基因在视网膜发育中的功能
- 批准号:
7885124 - 财政年份:2003
- 资助金额:
$ 34.49万 - 项目类别:
Function of the Six and Eya Genes in Retinal Development
Six 和 Eya 基因在视网膜发育中的功能
- 批准号:
6669280 - 财政年份:2003
- 资助金额:
$ 34.49万 - 项目类别:
Function of the Six and Eya Genes in Retinal Development
Six 和 Eya 基因在视网膜发育中的功能
- 批准号:
6781741 - 财政年份:2003
- 资助金额:
$ 34.49万 - 项目类别:
Function of the Six and Eya Genes in Retinal Development
Six 和 Eya 基因在视网膜发育中的功能
- 批准号:
8265287 - 财政年份:2003
- 资助金额:
$ 34.49万 - 项目类别:
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