Columbia AFM System Equipment Grant

哥伦比亚 AFM 系统设备补助金

• 批准号: 7794584
• 负责人: Clark T. Hung
• 金额: $ 49.64万
• 依托单位: COLUMBIA UNIV NEW YORK MORNINGSIDE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-22 至 2011-04-21
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7794584
• 关键词: Arts; Biological; Biological Sciences; Biomedical Engineering; Biomedical Research; Cells; Collaborations; Collection; Computer software; Core Facility; Equipment; Fluorescence Recovery After Photobleaching; Fostering; Grant; Head; Height; Home environment; Housing; Image; Interdisciplinary Study; Label; Lasers; Lead; Light; Maps; Materials Testing; Measurement; Mechanical Stimulation; Mechanics; Medical; Microscope; Microscopy; Molecular; Motivation; New York City; Physical environment; Request for Applications; Research; Research Personnel; Sampling; Scanning; Scanning Probe Microscopes; Structure; System; Time; Tissues; United States National Institutes of Health; Universities; base; catalyst; design; instrument; instrumentation; photoactivation; programs; response; single molecule; stem; tool; user-friendly

DESCRIPTION (provided by applicant): In response to PAR-09-028: Shared Instrumentation Grant Program (S10), this application requests support for a state-of-the-art BioScope Catalyst Atomic Force Microscope (AFM) system (Veeco Instruments, Plainview, NY) integrated with a new Olympus IX81 inverted microscope equipped with a Fluoview FV1000 confocal laser scanning system (LCSM, Olympus, Center Valley, PA) to be housed in the core facilities of the Department of Biomedical Engineering at Columbia University. Motivation for this request stems from the fact that there are currently no core facilities at Columbia University (or that we are aware of in New York City) that provide an AFM system with confocal capabilities for general life sciences applications. The AFM has become a staple instrument for biomedical engineering research, providing an important imaging and material testing tool spanning applications from single molecules to tissues. The integrated system will be used by multiple NIH- supported investigators for at least 85% of the time and will be made accessible for other researchers and academic purposes on an availability basis. The requested BioScope Catalyst system is user-friendly with integrated microscopy and analyses software (MIRO), as well as an open scan head design that permits simultaneous transmitted light applications, easy sample access and can be integrated with the FV1000 LSCM system. A microscope configuration combining these imaging sytems can be used to great advantage in cellular and tissue-level biomedical research where structures imaged with AFM can be cross-correlated to biomolecules identifiable via the LCSM. Similarly, using this system, AFM force measurements can permit mapping of sample mechanical stiffness to be correlated with underlying fluorescently labeled matrix structures visible by LSCM. Lastly, measurement of cellular responses using LCSM in response to AFM-induced mechanical stimulation provides unique opportunities for studies of mechanobiology, which investigates how cells perceive and respond to their physical environment. Further enhancing the capabilities of this integrated AFM system, the Fluoview FV1000 SIM (SIMultaneous) module provides photoactivation or bleaching and simultaneous image collection, enabling fluorescence recovery after photobleaching (FRAP) and molecular uncaging applications. Therefore, a research platform that delivers combined AFM and LSCM capabilities would offer a powerful research tool for biological applications. The BME department is home to the largest number of NIH-supported investigators at the downtown campus and is well-poised to lead this Shared Instrumentation effort, serving to further bridge the two campuses (downtown Morningside Heights to the uptown Medical Campus) and foster interdisciplinary research collaborations at Columbia.

DESCRIPTION (provided by applicant): In response to PAR-09-028: Shared Instrumentation Grant Program (S10), this application requests support for a state-of-the-art BioScope Catalyst Atomic Force Microscope (AFM) system (Veeco Instruments, Plainview, NY) integrated with a new Olympus IX81 inverted microscope equipped with a Fluoview FV1000 confocal laser scanning system （LCSM，Olympus，宾夕法尼亚州中心谷），位于哥伦比亚大学生物医学工程系的核心设施中。这一要求的动机源于以下事实：哥伦比亚大学目前没有核心设施（或我们在纽约市知道的）提供了一个为一般生命科学应用程序共同功能的AFM系统。 AFM已成为生物医学工程研究的主食工具，为从单分子到组织的应用提供了重要的成像和材​​料测试工具。该集成系统将至少在85％的时间内由多个支持NIH支持的研究人员使用，并可以在其他研究人员和学术目的上使用。请求的Bioscope催化剂系统具有用户友好的集成显微镜和分析软件（MIRO），以及允许同时传输的光应用程序，易于示例访问的开放扫描头设计，并且可以与FV1000 LSCM系统集成。结合这些成像系统的显微镜构型可用于在细胞和组织水平的生物医学研究中获得极大的优势，在细胞和组织水平的生物医学研究中，与AFM成像的结构可以通过LCSM识别的生物分子交叉相关。同样，使用该系统，AFM力测量可以允许映射样品机械刚度，以与LSCM可见的潜在荧光标记的矩阵结构相关。最后，使用LCSM响应AFM诱导的机械刺激来测量细胞反应为机械生物学研究提供了独特的机会，该研究研究了细胞如何感知和对其物理环境的反应。 Fluoview FV1000 SIM（同时）模块进一步增强了该集成的AFM系统的功能，提供了光激活或漂白和同时图像收集，从而在光漂白（FRAP）和分子不陈化应用后荧光恢复。因此，提供联合AFM和LSCM功能的研究平台将为生物应用提供强大的研究工具。 BME部门是市区校园中NIH支持的调查员数量最多的所在地，并进行了充分的努力，以领导这一共同的仪器工作，以进一步弥合两个校园（Morningside Morningside Heights到Uptown Medical Campus）和哥伦比亚哥伦比亚市培养跨学科研究合作。