UNMC SIG Supporting Next-generation DNA Sequencing Technology

描述（由申请人提供）： 项目摘要内布拉斯加大学医学中心（UNMC）正在申请资金购买Illumina基因组分析仪IIX（GAIIX），为UNMC生物医学研究界提供最先进的基因组服务。GAIIX仪器将作为一个核心设施，由训练有素、能力很强的科学家和技术人员安置和操作。这一安置和管理战略将确保仪器的有效和最大限度的使用，并确保需要仪器和相关技术应用的研究人员及时获得所需的支持。GAIIX是下一代DNA测序仪器，能够从各种不同的实验环境中生成千兆位（Gb）量的DNA核苷酸信息。该仪器将促进基因组研究，包括DNA测序以调查遗传变异，全基因组基因调控过程，包括蛋白质/ DNA相互作用以及表观遗传机制，以及各种全转录组应用，包括mRNA和miRNA测序和数字基因表达标签分析。目前，联合国军事视察员办公区还没有这种技术。该提案中概述的NIH支持的项目包括与乳腺癌、艾滋病、淋巴瘤、甲基苯丙胺滥用、传染病、神经系统疾病和神经变性、视觉和听觉神经感觉系统、语言和学习障碍以及呼吸系统疾病相关的生物医学分子研究。九个NIH支持的主要用户来自各种学术部门，研究所和中心，包括Eppley癌症研究所，自1982年以来NCI指定的癌症中心，淋巴瘤和白血病研究中心以及神经病毒学和神经退行性疾病中心。在UNMC生物医学研究界对下一代测序应用有相当大的兴趣，这一点可以从本提案中概述的9个主要用户和11个次要用户项目以及UNMC的许多其他研究人员也表示有兴趣使用该技术中得到证明。Ilumina GAIIX仪器和下一代测序服务的本地可用性将对UNMC和整个当地生物医学研究界的许多不同研究项目产生重大影响，从而加强研究并促进研究人员有效竞争NIH和其他资助机构现有和新的赠款申请的能力。

DESCRIPTION (provided by applicant): Project Summary/Abstract The University of Nebraska Medical Center (UNMC) is requesting funds to purchase an Illumina Genome Analyzer IIX (GAIIX) to provide state-of-the-art genomic services to the UNMC biomedical research community. The GAIIX instrument will be housed and operated as a core facility by well-trained and highly capable scientists and technical personnel. This placement and management strategy will ensure the efficient and maximal use of the instrument, as well as ensure that the researchers needing the instrument and associated technological applications have the support they need in a timely fashion. The GAIIX is a next- generation DNA sequencing instrument capable of generating gigabase (Gb) quantities of DNA nucleotide information from a variety of different experimental contexts. The instrument will facilitate genomic research involving DNA sequencing to investigate genetic variation, genome-wide gene regulatory processes to include protein / DNA interactions as well as epigenetic mechanisms, and a variety of whole transcriptome applications to include mRNA and miRNA sequencing and digital gene expression-tag profiling. This technology is currently unavailable on the UNMC campus. The NIH supported projects outlined in this proposal include molecular studies of biomedical relevance to breast cancer, HIV disease, lymphoma, methamphetamine abuse, infectious disease, neurological disorders and neurodegeneration, neurosensory systems of vision and hearing, disorders of language and learning, and respiratory disorders. The nine NIH supported major users come from a variety of academic departments, institutes, and centers including the Eppley Cancer Institute, an NCI designated cancer center since 1982, the Center for Lymphoma and Leukemia Research, and the Center for Neurovirology and Neurodegenerative Diseases. There is considerable interest in next-generation sequencing applications in the UNMC biomedical research community as evidenced by the nine major user and eleven minor user projects outlined in this proposal, as well as many other researchers here at UNMC that have also expressed an interest in using the technology. The local availability of the Ilumina GAIIX instrument and next-generation sequencing services will have a major impact on a number of different research programs at UNMC and the local biomedical research community as a whole, thus enhancing research and facilitating the ability of researchers to compete effectively for existing and new grant applications at NIH and other funding agencies.