Molecular Biology Shared Resource

项目摘要：分子生物学共享资源 分子生物学共享资源(MBSR)创建于2009年，旨在将其整合为一个 运行基因组学设施和分子生物学/高通量筛选(MB/HTS)设施。这个 合并加强了核心之间的沟通，最大限度地利用了工作人员的专业知识，以及 提高了所有癌症中心项目代表使用的服务质量。MBSR提供：1) 下一代测序(Illumina)、单细胞基因组学(10x基因组学)、纳米串靶向基因 表达分析和Sanger测序；2)全基因组siRNA和小分子化学物质(&gt；100K) HTS筛选，3)高含量成像和分析，以及4)使用Luminex xMAP进行多分析物分析 技术。该资源为每个研究人员提供关于实验设计的免费咨询， 预算估算、结果和数据评估，是分子生物试剂的重要资源， 信息，以及分子生物技术和分析软件方面的培训。MBSR位于 指导和监督管理机制日常活动的两名联席主任。在这方面， 大卫·凯利博士负责监督位于BCC的MB/HTS设施，詹姆斯·尤迪博士负责监督基因组学 位于达勒姆研究中心II的设施，都位于UNMC校园内。六名技术人员是 受雇于MBSR，人员接受过广泛的交叉培训，以执行几乎所有服务和操作 几乎所有的仪器和设备。MBSR是使用最广泛的BCC共享资源之一， 支持UNMC研究界的184个生物医学实验室，其中约53% 在BCC研究计划中由BCC调查人员领导的实验室(靶标、探针和交付， 癌症生物学计划和胃肠道癌症计划)。满足和满足快速变化的需求 通知BCC调查人员，MBSR人员举办研讨会，在当地研讨会上发言，参加癌症 中心项目会议和多研究员项目会议，并出席实验室会议。 此外，他们还参加国家和地区技术会议，学习最新技术， 技术和方法。

PROJECT SUMMARY: MOLECULAR BIOLOGY SHARED RESOURCE The Molecular Biology Shared Resource (MBSR) was created in 2009 to bring together into one consolidated operation the Genomics Facility and the Molecular Biology/High-Throughput Screening (MB/HTS) Facility. The merger enhanced the communication between the cores, maximized the utilization of staff expertise, and raised the quality of services used by representatives of all Cancer Center programs. The MBSR offers: 1) next-generation sequencing (Illumina), single-cell genomics (10xGenomics), NanoString targeted gene expression assays, and Sanger sequencing; 2) genome-wide siRNA and small-molecule chemical (>100K) HTS screening, 3) high-content imaging and analysis, and 4) multi-analyte profiling using Luminex xMAP technologies. The resource provides free consultation to every researcher regarding experimental design, budget estimates, results, and data assessment, and is a significant resource for molecular biological reagents, information, and training in molecular biological techniques and analysis software. The MBSR is under the direction and oversight of two Co-Directors who manage the day-to-day activities of the Facility. In this regard, Dr. David Kelly oversees the MB/HTS Facility located in the BCC, and Dr. James Eudy oversees the Genomics Facility located in the Durham Research Center II, both on the UNMC campus. Six technical staff members are employed in the MBSR with personnel extensively cross-trained to perform virtually all services and to operate virtually all instrumentation and equipment. The MBSR is one of the most widely used BCC Shared Resource, supporting over 184 biomedical laboratories in the UNMC research community, of which approximately 53% are laboratories led by BCC investigators in the BCC research programs (Targets, Probes, and Delivery, Cancer Biology Program, and Gastrointestinal Cancer Program). To meet the rapidly changing needs of and to inform BCC investigators, the MBSR personnel conduct workshops, speak at local seminars, attend Cancer Center program meetings and multi-investigator project meetings, and present at laboratory meetings. Moreover, they attend national and regional technology conferences to learn the latest technologies, techniques, and methods.