Molecular Biology Shared Resource

分子生物学共享资源

• 批准号: 10270916
• 负责人: James Eudy
• 金额: $ 12.93万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-05 至 2026-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10270916
• 关键词: Biological; Biological Assay; Budgets; Cancer Biology; Cancer Center; Cancer Center Support Grant; Cells; Chemicals; Communication; Communities; Computer software; Consultations; DNA sequencing; Data; Dideoxy Chain Termination DNA Sequencing; Educational workshop; Effectiveness; Equipment; Experimental Designs; Faculty; Gene Expression Profiling; Genes; Genomics; Human Resources; Image; Infrastructure; Laboratories; Lead; Learning; Malignant neoplasm of gastrointestinal tract; Mentors; Metagenomics; Methods; Mission; Molecular; Molecular Biology; Molecular Biology Shared Resource; Organism; Performance; Population; Postdoctoral Fellow; Procedures; Process; RNA; Reagent; Research; Research Personnel; Research Project Grants; Resource Sharing; Resources; Ribosomes; Services; Small Interfering RNA; Students; Techniques; Technology; Tissues; Training; Update; Validation; assay development; cellular imaging; cost; design; exome; functional genomics; genome sequencing; genome-wide; graduate student; high throughput screening; instrumentation; lectures; mRNA sequencing; meetings; member; miRNA expression profiling; nano-string; next generation; next generation sequencing; operation; programs; screening; skills training; small molecule; symposium; targeted sequencing; technology/technique; transcriptome sequencing; virtual; whole genome

PROJECT SUMMARY: MOLECULAR BIOLOGY SHARED RESOURCE The Molecular Biology Shared Resource (MBSR) was created in 2009 to bring together into one consolidated operation the Genomics Facility and the Molecular Biology/High-Throughput Screening (MB/HTS) Facility. The merger enhanced the communication between the cores, maximized the utilization of staff expertise, and raised the quality of services used by representatives of all Cancer Center programs. The MBSR offers: 1) next-generation sequencing (Illumina), single-cell genomics (10xGenomics), NanoString targeted gene expression assays, and Sanger sequencing; 2) genome-wide siRNA and small-molecule chemical (>100K) HTS screening, 3) high-content imaging and analysis, and 4) multi-analyte profiling using Luminex xMAP technologies. The resource provides free consultation to every researcher regarding experimental design, budget estimates, results, and data assessment, and is a significant resource for molecular biological reagents, information, and training in molecular biological techniques and analysis software. The MBSR is under the direction and oversight of two Co-Directors who manage the day-to-day activities of the Facility. In this regard, Dr. David Kelly oversees the MB/HTS Facility located in the BCC, and Dr. James Eudy oversees the Genomics Facility located in the Durham Research Center II, both on the UNMC campus. Six technical staff members are employed in the MBSR with personnel extensively cross-trained to perform virtually all services and to operate virtually all instrumentation and equipment. The MBSR is one of the most widely used BCC Shared Resource, supporting over 184 biomedical laboratories in the UNMC research community, of which approximately 53% are laboratories led by BCC investigators in the BCC research programs (Targets, Probes, and Delivery, Cancer Biology Program, and Gastrointestinal Cancer Program). To meet the rapidly changing needs of and to inform BCC investigators, the MBSR personnel conduct workshops, speak at local seminars, attend Cancer Center program meetings and multi-investigator project meetings, and present at laboratory meetings. Moreover, they attend national and regional technology conferences to learn the latest technologies, techniques, and methods.

项目概要：分子生物学共享资源 分子生物学共享资源（MBSR）创建于2009年，旨在将其整合为一个统一的资源库。 运行基因组学设施和分子生物学/高通量筛选（MB/HTS）设施。的 合并加强了核心之间的沟通，最大限度地利用了工作人员的专门知识， 提高了所有癌症中心项目代表使用的服务质量。MBSR提供：1） 下一代测序（Illumina）、单细胞基因组学（10 xGenomics）、NanoString靶向基因 表达测定和桑格测序; 2）全基因组siRNA和小分子化学（> 100 K） HTS筛查，3）高含量成像和分析，以及4）使用Luminex xMAP的多分析物分析 技术.该资源为每一位研究人员提供关于实验设计的免费咨询， 预算估计，结果和数据评估，是分子生物学试剂的重要资源， 分子生物学技术和分析软件方面的培训。正念减压疗法是根据 指导和监督两名共同主任，负责管理基金的日常活动。在这方面，委员会注意到， 博士大卫凯利监督位于BCC的MB/HTS设施，詹姆斯Eudy博士监督基因组学 设施位于达勒姆研究中心二，都在UNMC校园。6名技术人员 在MBSR中雇用的人员经过广泛的交叉培训，几乎可以执行所有服务并操作 几乎所有的仪器和设备。MBSR是最广泛使用的BCC共享资源之一， 支持UNMC研究界超过184个生物医学实验室，其中约53% 是由BCC研究人员在BCC研究计划（目标，探针和交付， 癌症生物学计划和胃肠道癌症计划）。为了满足快速变化的需求， 通知BCC研究人员，MBSR人员举办研讨会，在当地研讨会上发言，参加癌症 中心项目会议和多研究者项目会议，并出席实验室会议。 此外，他们还参加国家和区域技术会议，学习最新技术， 技术和方法。