Fluorometric Imaging Plate Reader (FLIPRtetra)

描述（由申请人提供）：细胞膜受体利用许多细胞内第二信使将其信号传播到细胞内。单个受体可以激活许多细胞内信号通路。一种能够实时详细检查一个特定信号通路或同时以高通量方式检查多个通路的仪器可以成为基础研究的宝贵资产。荧光成像板阅读器（flir1）最初设计用于利用荧光染料监测细胞膜电位的变化。随后，FLIPR1被用于监测细胞内钙的快速变化。FLIPR1已被证明是南卡罗来纳医科大学研究人员的一个有价值的调查工具。它的年龄加上目前缺乏制造商的支持，使FLIPR1处于过时的边缘。最新版本的flir， FLIPRtetra，扩展了原始仪器的基本概念。FLIPRtetra具有96、384和1536孔配置，多波长动力学读数，LED模块和用户可配置的激发和发射滤波器，可选适配器，用于添加细胞悬浮液，可选aequorin发光相机增强发光灵敏度。通过用户可配置的LED激发模块，FLIPRtetra扩展了染料的功能，包括：比例电压传感、线粒体膜去极化、细胞内谷胱甘肽水平、活性氧等。FLIPRtetra将为美国国立卫生研究院资助的研究人员提供一种新的工具来检查无数的细胞内信号系统，并为他们提供新的信息，使他们能够扩展他们的研究和知识。

DESCRIPTION (provided by applicant): Cell membrane receptors utilize numerous intracellular second messengers to propagate their signal into and through the cell. Individual receptors can activate many intracellular signaling pathways. An instrument capable of examining in real time either one specific signal pathway in detail or multiple pathways at the same time in a high throughput manner can be a valued asset to basic research. The Fluorometric Imaging Plate Reader (FLIPR 1) was originally designed to utilize fluorescent dyes to monitor changes in cell membrane potential. Subsequently the FLIPR1 was used to monitor rapid intracellular changes in calcium. The FLIPR1 has proven to be a valuable investigative tool for the investigators at the Medical University of South Carolina. Its age along with the lack of current support from the manufacturer has put the FLIPR1 in a condition bordering on obsolescence. The newest version of the FLIPR, FLIPRtetra, has expanded on the basic concepts of the original instrument. FLIPRtetra, features 96, 384 and 1536 well configurations, multi-wavelength kinetic readings, LED modules and user configurable excitation and emission filters, an optional adapter for addition of cell suspensions and enhanced luminescence sensitivity with the optional aequorin luminescence camera. With the user configurable LED excitation modules, the FLIPRtetra has expanded dye capabilities to include: ratiometric voltage sensing, mitochondrial membrane de-polarization, intracellular glutathione levels, reactive oxygen species and others. The FLIPRtetra will provide NIH-funded investigators a new tool to examine a myriad of intracellular signal systems and provide them with new information that will allow them to expand their research and knowledge.