Immunology and Pathology Core

免疫学和病理学核心

• 批准号: 7883574
• 负责人: Julie A Lovchik
• 金额: $ 32.45万
• 依托单位: UNIVERSITY OF NEW MEXICO
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7883574
• 关键词: Animal Model; Anthrax disease; Blood; Categories; Cell Count; Cell Culture Techniques; Cells; Collecting Cell; Data; Detection; Elements; Evaluation; Flow Cytometry; Goals; Human; Human Resources; Immune response; Immunohistochemistry; Immunology; Infection; Laboratories; Liquid substance; Lung; Messenger RNA; Methodology; Methods; Modeling; Mus; Organ; Pathology; Phenotype; Population Analysis; Preparation; Primates; Procedures; Proteins; RNA; Reliance; Reverse Transcriptase Polymerase Chain Reaction; Sampling; Science; Serum; Specimen; Staining method; Stains; Techniques; Time; Tissue Sample; Tissues; Tularemia; animal tissue; cytokine; enzyme linked immunospot assay; high throughput analysis; nonhuman primate; novel therapeutics; pathogen; programs; response; therapeutic vaccine; tissue culture; tissue processing

This Immunology and Pathology Laboratory Core (Core B) will serve as a centralized facility for procedures common to all of the projects in the Program Project. This core will have the capacity to perform high throughput analysis of samples generated from animals and tissue culture. The core will perform multiplexing analysis of cytokine proteins from various samples generated from serum and cell culture. It will also perform RT-PCR for detection mRNA cytokines isolated from various tissues as needed. Finally, it will perform routine and specialized immunohistochemistry on tissue samples for examination of pathological specimens. It wil also assist with sample preparation from various tissues. All three projects will use this core for some element of their analysis. This Core will be the most heavily used for the PPG. Specifically this Immunology and Pathology core will: 1) Provide standardized methods for evaluating the level of cytokine proteins and/or their RNA in fluids and tissues collected from mice, human cells or primates. Methods will include multiplexing strategies for quantitatively detecting multiple cytokines in one sample, real-time PCR techniques for quantitative detection of cytokine RNAs, ELISpot methods to determine the number of cells collected from either organs or blood producing specific cytokines 2) Provide capability in increased labor capacity to prepare samples from mice and primates for histological and cellular analysis 3) Provide capability and capacity in assisting in disruption of lung cells and evaluation of cell phenotypes of cells recovered directly from organs or tissue culture by flow cytometry techniques. Other capabilities will be to perform intracellular cytokine staining for phenotyping cells recovered animal tissues and blood or potentially tissue culture as needed.

这个免疫学和病理学实验室核心(核心B)将作为计划项目中所有项目通用程序的集中设施。该核心将具有对动物和组织培养产生的样本进行高通量分析的能力。该核心将对血清和细胞培养产生的各种样本中的细胞因子蛋白进行多路分析。它还将根据需要进行RT-PCR，以检测从各种组织中分离出的mRNA细胞因子。最后，它将对组织样本进行常规和专门的免疫组织化学检查，以检查病理标本。它还将协助从各种组织中制备样品。所有这三个项目都将使用这个核心来进行分析。这个核心将是PPG使用最多的核心。具体地说，这一免疫学和病理学核心将：1)提供标准化方法，用于评估从小鼠、人类细胞或灵长类动物收集的液体和组织中细胞因子蛋白和/或其RNA的水平。方法包括定量检测一个样本中的多种细胞因子的多重策略、定量检测细胞因子RNA的实时聚合酶链式反应技术、确定从器官或血液中收集的产生特定细胞因子的细胞数量的ELISpot方法 2)提供提高劳动能力的能力，以制备小鼠和灵长类动物的样本，用于组织学和细胞分析3)提供能力和能力，协助破坏肺细胞，并利用流式细胞仪技术评估直接从器官或组织培养中回收的细胞的细胞表型。其他能力将是根据需要对表型细胞、恢复的动物组织和血液或潜在的组织培养进行细胞内细胞因子染色。