Immunology and Pathology Core

免疫学和病理学核心

• 批准号: 6857537
• 负责人: Julie A Lovchik
• 金额: $ 32.9万
• 依托单位: UNIVERSITY OF NEW MEXICO
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6857537
• 关键词: RNA; animal tissue; biomedical facility; bioterrorism /chemical warfare; cytokine; disease /disorder model; enzyme linked immunosorbent assay; flow cytometry; immunocytochemistry; nucleic acid quantitation /detection; polymerase chain reaction; protein quantitation /detection; respiratory infections

This Immunology and Pathology Laboratory Core (Core B) will serve as a centralized facility for procedures common to all of the projects in the Program Project. This core will have the capacity to perform high throughput analysis of samples generated from animals and tissue culture. The core will perform multiplexing analysis of cytokine proteins from various samples generated from serum and cell culture. It will also perform RT-PCR for detection mRNA cytokines isolated from various tissues as needed. Finally, it will perform routine and specialized immunohistochemistry on tissue samples for examination of pathological specimens. It wil also assist with sample preparation from various tissues. All three projects will use this core for some element of their analysis. This Core will be the most heavily used for the PPG. Specifically this Immunology and Pathology core will: 1) Provide standardized methods for evaluating the level of cytokine proteins and/or their RNA in fluids and tissues collected from mice, human cells or primates. Methods will include multiplexing strategies for quantitatively detecting multiple cytokines in one sample, real-time PCR techniques for quantitative detection of cytokine RNAs, ELISpot methods to determine the number of cells collected from either organs or blood producing specific cytokines 2) Provide capability in increased labor capacity to prepare samples from mice and primates for histological and cellular analysis 3) Provide capability and capacity in assisting in disruption of lung cells and evaluation of cell phenotypes of cells recovered directly from organs or tissue culture by flow cytometry techniques. Other capabilities will be to perform intracellular cytokine staining for phenotyping cells recovered animal tissues and blood or potentially tissue culture as needed.

该免疫学和病理学实验室核心（核心B）将作为计划项目中所有项目通用程序的集中设施。该核心将有能力对动物和组织培养产生的样品进行高通量分析。核心将对血清和细胞培养物产生的各种样品中的细胞因子蛋白进行多重分析。根据需要，还将进行RT-PCR以检测从各种组织中分离的mRNA细胞因子。最后，对组织样本进行常规和专业免疫组化，用于病理标本的检查。它还将有助于从各种组织中制备样品。所有三个项目都将使用该核心进行某些分析。该核心将是PPG使用最多的核心。具体而言，该免疫学和病理学核心将：1）提供标准化方法，用于评估从小鼠、人类细胞或灵长类动物收集的液体和组织中细胞因子蛋白和/或其RNA的水平。方法将包括用于定量检测一个样品中多种细胞因子的多重策略、用于定量检测细胞因子RNA的实时PCR技术、用于确定从器官或血液收集的产生特异性细胞因子的细胞数量的ELISpot方法 2)3）提供协助破坏肺细胞和通过流式细胞术技术评价直接从器官或组织培养物回收的细胞的细胞表型的能力。其他能力将是进行细胞内细胞因子染色，用于表型细胞、回收的动物组织和血液或可能的组织培养（如需要）。