NUTRITIONAL AND CONTRACTILE REGULATION OF MUSCLE GROWTH

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 背景：这个项目的重点是探索肌肉如何生长。 肌肉萎缩是许多疾病中的常见现象，例如癌症、艾滋病、创伤、败血症、肾衰竭，并且在老年人中也特别普遍。 具体目的和程序（概述）：（1）确定在一次抗阻运动之前或之后给予食物时肌肉生长对进食的短期反应的大小。 (2)确定肌肉生长对食物的短期反应的大小，这些食物提供的能量与锻炼期间使用的能量相匹配或超过。 (3)确定女性和男性之间的差异，肌肉生长的短期反应举重单独和结合喂养时，摄入之前或之后一轮举重运动。 我们将使用的程序，以解决这些具体的目标，包括使用股导管，举重运动，血液和呼吸收集和肌肉活检。 在每次实验中，我们将持续注入稳定的同位素。 稳定同位素没有放射性，可以安全地给予人类。 这些稳定的同位素使我们能够测量人类短期的肌肉生长。 意义（总结）：这些研究将深入了解调节人类肌肉生长的机制，并将作为开发干预措施的基础，以改善衰老，创伤，癌症和艾滋病等条件下的肌肉生长。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Background: The focus of this project is to explore how muscles grow. Muscle wasting is a common phenomenon in many conditions, such as cancer, AIDS, trauma, sepsis, kidney failure, and is also particularly prevalent in the elderly. Specific Aims and Procedures (summary): (1) To determine the magnitude of the short-term response of muscle growth to feeding when the food is given before or after a bout of resistance exercise. (2) To determine the magnitude of the short-term response of muscle growth to food that provides an amount of energy that either matches or exceeds the amount of energy used during the exercise. (3) To determine the differences between women and men in the short-term response of muscle growth to weight lifting alone and in combination with feeding when ingested before or after a bout of weight lifting exercise. The procedures we will use in order to address these specific aims include the use of femoral catheters, weight lifting exercise, blood and breath collection and taking muscle biopsies. During each experiment we will be continuously infusing stable isotopes. Stable isotopes are not radioactive and can be given safely to humans. These stable isotopes allow us to be able to measure short-term muscle growth in humans. Significance (summary): These studies will provide insight into the mechanisms that regulate human muscle growth, and will be utilized as a basis from which to develop interventions for improving muscle growth in conditions such as aging, trauma, cancer, and AIDS.