BIOCHEMICAL MARKERS IN ADULT PATIENTS WITH ANEURYSMAL SUBARACHNOID HEMORRHAGE

动脉瘤性蛛网膜下腔出血成年患者的生化标志物

• 批准号: 7717128
• 负责人: stephen john lewis
• 金额: $ 0.8万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-12-01 至 2008-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7717128
• 关键词: Acute Brain Injuries; Adult; Aneurysmal Subarachnoid Hemorrhages; Apoptotic; Biochemical Markers; Biological; Biological Assay; Biological Markers; Blood specimen; Case Fatality Rates; Cell Death; Cerebral Ischemia; Cerebrospinal Fluid; Cerebrovascular Spasm; Cerebrum; Classification; Computer Retrieval of Information on Scientific Projects Database; Diagnostic; Enzyme-Linked Immunosorbent Assay; Event; Funding; Goals; Grant; Hemorrhage; Human; Injury; Institution; Liquid substance; Morbidity - disease rate; Myelin Basic Proteins; Neurologic; Outcome; Patients; Research; Research Personnel; Resources; Sensitivity and Specificity; Serum; Source; Spectrin; Stroke; Subarachnoid Hemorrhage; System; United States National Institutes of Health; Validation; Vasospasm; mortality; prognostic; tau Proteins

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Aneurysmal subarachnoid hemorrhage ASAH is a serious event with a 51% case fatality rate. Neurological complications following ASAH are common. Delayed ischemic neurological deficit caused by cerebral arterial vasospasm contributes significantly to morbidity and mortality. Biomarkers may provide early warning of impending neurological decline and stroke from ASAH induced vasospasm. This proposal would initiate a systematic identification and validation of a panel of biochemical markers for acute brain injury resulting from the effects of ASAH, detectable in human cerebrospinal fluid CSF and serum, biological fluids routinely accessible following SAH. Specific Aim 1 assesses the diagnostic and prognostic utility of candidate biomarkers breakdown products to spectrin-SBDPs; tau; UCH-L1; MAP2a/b; myelin basic protein-MBP; S100B by examining relationships between levels of specific biomarkers and injury magnitude e.g. WFNS grading scale, magnitude of hemorrhage e.g. Fisher CT grading, occurrence of secondary ischemia from cerebral vasospasm and outcome e.g. neurological assessments. Sensitivity and specificity of markers, alone and in combination will be determined. Specific Aim 2 assesses the utility of these biomarkers for assessment of oncotic and apoptotic cell death mechanisms by examining relationships between CSF levels of biomarkers and specific pathological events that occur after ASAH. The long-term goal of this research is to produce quantitative ELISA assay systems capable of providing clinically useful diagnostic and prognostic information from both CSF and blood samples.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 动脉瘤性蛛网膜下腔出血ASAH是一种严重事件，病死率为51%。 ASAH后的神经系统并发症很常见。 脑动脉痉挛引起的迟发性缺血性神经功能缺损是导致发病率和死亡率的重要原因。 生物标志物可以提供早期预警即将发生的神经功能下降和中风的ASAH诱导的血管痉挛。 该提案将启动一个系统的识别和验证的生化标志物的面板的急性脑损伤所造成的ASAH的影响，可检测的人脑脊液CSF和血清，生物流体常规访问后SAH。特异性目的1评估了候选生物标志物分解产物对血影蛋白-SBDP; tau; UCH-L1; MAP 2a/B;髓鞘碱性蛋白-MBP; S100 B通过检查特定生物标志物的水平与损伤程度（例如WFNS分级量表）、出血程度（例如Fisher CT分级）、脑血管痉挛继发性缺血的发生率和结局（例如神经学评估）之间的关系。将确定标记物单独和组合的灵敏度和特异性。具体目标2通过检查CSF生物标志物水平与ASAH后发生的特定病理事件之间的关系，评估这些生物标志物用于评估肿瘤和凋亡细胞死亡机制的效用。 这项研究的长期目标是生产定量ELISA检测系统，能够提供临床有用的诊断和预后信息，从CSF和血液样本。