Multiplex Mass Spectrometric Protein Assays for Precise Monitoring of the Pathophysiology of Obesity
用于精确监测肥胖病理生理学的多重质谱蛋白质分析
基本信息
- 批准号:10238054
- 负责人:
- 金额:$ 75.28万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2019
- 资助国家:美国
- 起止时间:2019-09-20 至 2023-07-31
- 项目状态:已结题
- 来源:
- 关键词:AdoptedAdultAffinityAnti-Inflammatory AgentsAreaBenchmarkingBiochemistryBiological AssayBiological MarkersBlindedBlood ProteinsBody Weight decreasedBuffaloesC-reactive proteinCardiovascular DiseasesChildClinicalCollaborationsCommunitiesCoupledDataDetectionDiabetes MellitusDiseaseEpidemicFailureFamilyFunctional disorderGDF15 geneGoalsHormonesIGF1 geneImmunoassayInflammatoryInsulinInsulin ResistanceInsulin-Like Growth-Factor-Binding ProteinsIntelligenceInterventionLCN2 geneLaboratoriesLeptinMass Spectrum AnalysisMatrix MetalloproteinasesMedicineMetabolismMonitorNon-Insulin-Dependent Diabetes MellitusObesityOverweightPatient CarePeptidesPerformancePhasePlasmaPrevalenceProceduresProtein IsoformsProteinsProtocols documentationRBP4 geneReagentReportingReproducibilityResearchResearch InstituteResolutionSamplingSiteSolidSpecificityStandardizationTechnologyTranslational ResearchTranslationsUniversitiesValidationVermontWeightWorkadipokinesadiponectinassay developmentbasebiomarker panelblood glucose regulationcancer typeclinical applicationcohortcombatdata repositorydisorder preventionenergy balanceexperienceexperimental studyghrelininflammatory markerinsightmortality riskmultiplex assaymultiplex detectionobese personpersonalized carepersonalized medicineprecision medicinepressureprohormoneresistance exercisevalidation studies
项目摘要
PROJECT SUMMARY/ABSTRACT
The prevalence of overweight and obesity has increased dramatically in recent decades in both children and
adults, reaching an epidemic proportion. In order to gain new insights into the pathophysiology of obesity
towards better personalized patient care, there is a critical need to develop reliable multiplex protein assays that
can be easily implemented in clinical laboratories to enable precise monitoring of many hormones and
inflammatory markers closely associated with obesity. Unfortunately, current clinical assays often lack of
reproducibility, especially between different clinical laboratories due to the lack of assay standardization.
Targeted mass spectrometry is a promising technology for providing robust multiplex assays for blood proteins.
Therefore, the overall objective of this application is to develop and validate reproducible and transferable mass
spectrometry-based multiplex protein assays for enabling precise quantification of a panel of obesity markers.
The panel will cover multiple aspects of obesity pathophysiology, including glucose homeostasis and energy
balance (e.g., insulin, leptin, and other hormones), pro-inflammatory markers (e.g., C-reactive protein), and
anti-inflammatory markers (e.g., adiponectin). To facilitate full validation of the robustness and transferability
of the assays, an inter-lab assay validation will be pursued. Specifically, Aim 1 will be focused on initial assay
development for optimal configurations and on demonstrating confident multiplex detection of endogenous
analytes in blood plasma. Aim 2 will be centered on assay optimization and full assay characterization in the
aspects of reproducibility, stability, selectivity, linearity, and limit of quantification. Aim 3 will demonstrate the
utility of the assays through inter-lab quantification of endogenous analytes in a pilot cohort of clinical plasma
samples from normal weight and obese subjects, and obese individual before and after weight loss and
benchmarking against well-established immunoassays for selected analytes such as insulin and leptin. To
facilitate the easy- implementation of the validated assays in other laboratories, all assay data including
chromatographic data and detailed standard operating procedures will be shared through public data
repositories and assay portals. Together, the project will establish robust and easy-to-transfer multiplex assays
that will enable precise monitoring of the pathophysiology of obesity.
项目总结/文摘
项目成果
期刊论文数量(0)
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科研奖励数量(0)
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Wei-Jun Qian其他文献
Wei-Jun Qian的其他文献
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{{ truncateString('Wei-Jun Qian', 18)}}的其他基金
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10730900 - 财政年份:2023
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10395957 - 财政年份:2019
- 资助金额:
$ 75.28万 - 项目类别:
Multiplex Mass Spectrometric Protein Assays for Precise Monitoring of the Pathophysiology of Obesity
用于精确监测肥胖病理生理学的多重质谱蛋白质分析
- 批准号:
9918021 - 财政年份:2019
- 资助金额:
$ 75.28万 - 项目类别:
Reverse Sensitivity Analysis for Identifying Predictive Proteomics Signatures of Cancer
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- 资助金额:
$ 75.28万 - 项目类别:
Multiplex Mass Spectrometric Protein Assays for Precise Monitoring of the Pathophysiology of Obesity
用于精确监测肥胖病理生理学的多重质谱蛋白质分析
- 批准号:
10448306 - 财政年份:2019
- 资助金额:
$ 75.28万 - 项目类别:
Reverse Sensitivity Analysis for Identifying Predictive Proteomics Signatures of Cancer
用于识别癌症预测蛋白质组学特征的反向敏感性分析
- 批准号:
10615630 - 财政年份:2019
- 资助金额:
$ 75.28万 - 项目类别:
Multiplex Mass Spectrometric Protein Assays for Precise Monitoring of the Pathophysiology of Obesity
用于精确监测肥胖病理生理学的多重质谱蛋白质分析
- 批准号:
10020391 - 财政年份:2019
- 资助金额:
$ 75.28万 - 项目类别:
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