Close-loop, spatially addressable multiphoton functional imaging

闭环、空间可寻址多光子功能成像

基本信息

  • 批准号:
    10246271
  • 负责人:
  • 金额:
    $ 60.95万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2019
  • 资助国家:
    美国
  • 起止时间:
    2019-09-15 至 2023-08-31
  • 项目状态:
    已结题

项目摘要

Abstract A major goal of brain research is to image the dynamics of groups of neurons during behavior. Although even the simplest behaviors involve interactions across multiple parts of the nervous system, our tools for assessing function at the level of individual neurons usually allow only access to small regions of the brain, and with limited temporal resolution. Optical recordings of activity are critical to probe neural systems because they provide high-resolution, non-invasive measurements, ranging from single neurons to entire populations in intact nervous systems, and are readily combined with genetic methods to provide cell type-specific recordings. Nevertheless, the limited spatial scale and temporal resolution remain a major challenge for optical imaging. Cellular-resolution imaging in scattering brains is typically achieved with multiphoton microscopy (MPM). The focus of this proposal is to develop, implement, and disseminate a new generation of multiphoton imaging tools and genetically encoded indicators that allow deep, fast, and large-scale imaging of structure and function with cellular and subcellular resolution. To approach fundamental limits defined by the 'photon budget', we will develop an adaptive excitation source (AES). By feeding the structural information of the sample to the source, and synchronizing the on-demand pulses with the microscope scanning system, the AES transforms a conventional MPM into a “random-access” MPM that only excites regions of interest. We will integrate the AES with high speed scanners, resulting in a new AES-MPM that will provide >10x improvement in imaging speed or the number of neurons imaged. We will combine the AES effort at Cornell with the development effort of genetically encoded voltage indicator (GEVI) at Stanford and Janelia Research Campus, and demonstrate the AES-MPM in imaging GEVI labeled neurons in mouse brains in vivo. We will also combine the AES with a multiphoton mesoscope to demonstrate large scale imaging of genetically encoded Ca indicator (GECI). The research involves close interactions between the PI (Xu), Co-investigator Michael Lin (Stanford), and Karel Svoboda (Janelia). Furthermore, the investigators will work closely with industrial partners to explore the potential of translating the AES into a commercially available system, which provides a direct path to broad dissemination.
摘要

项目成果

期刊论文数量(0)
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会议论文数量(0)
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CHRIS XU其他文献

CHRIS XU的其他文献

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{{ truncateString('CHRIS XU', 18)}}的其他基金

Improving the Speed of Galvo-Scanners
提高振镜扫描仪的速度
  • 批准号:
    10616930
  • 财政年份:
    2023
  • 资助金额:
    $ 60.95万
  • 项目类别:
A multi-foci objective lens for large scale brain activity recording
用于大规模大脑活动记录的多焦点物镜
  • 批准号:
    10731905
  • 财政年份:
    2023
  • 资助金额:
    $ 60.95万
  • 项目类别:
Understanding the in vivo impact of immunotherapies in splenic lymphoma by intravital three-photon microscopy
通过活体三光子显微镜了解免疫疗法对脾淋巴瘤的体内影响
  • 批准号:
    10576013
  • 财政年份:
    2023
  • 资助金额:
    $ 60.95万
  • 项目类别:
Deep and fast imaging using adaptive excitation sources
使用自适应激励源进行深度快速成像
  • 批准号:
    10516870
  • 财政年份:
    2022
  • 资助金额:
    $ 60.95万
  • 项目类别:
Close-loop, spatially addressable multiphoton functional imaging
闭环、空间可寻址多光子功能成像
  • 批准号:
    10580393
  • 财政年份:
    2022
  • 资助金额:
    $ 60.95万
  • 项目类别:
Wavefront sensor for deep imaging of the brain
用于大脑深度成像的波前传感器
  • 批准号:
    9136863
  • 财政年份:
    2015
  • 资助金额:
    $ 60.95万
  • 项目类别:
Optimization of 3-photon microscopy for Large Scale Recording in Mouse Brain
用于小鼠大脑大规模记录的三光子显微镜优化
  • 批准号:
    8827026
  • 财政年份:
    2014
  • 资助金额:
    $ 60.95万
  • 项目类别:
Optimization of 3-photon microscopy for Large Scale Recording in Mouse Brain
用于小鼠大脑大规模记录的三光子显微镜优化
  • 批准号:
    9130300
  • 财政年份:
    2014
  • 资助金额:
    $ 60.95万
  • 项目类别:
Technology development for in vivo deep tissue imaging
体内深层组织成像技术开发
  • 批准号:
    8271179
  • 财政年份:
    2012
  • 资助金额:
    $ 60.95万
  • 项目类别:
Technology development for in vivo deep tissue imaging
体内深层组织成像技术开发
  • 批准号:
    8604711
  • 财政年份:
    2012
  • 资助金额:
    $ 60.95万
  • 项目类别:

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