Technology development for in vivo deep tissue imaging

体内深层组织成像技术开发

基本信息

批准号：
8271179
负责人：
CHRIS XU
金额：
$ 49.68万
依托单位：
CORNELL UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-04-01 至 2016-01-31
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): The goal of this research program is to explore new laser technologies, new spectral windows, and three- photon fluorescence microscopy for imaging deep into scattering tissues, and then demonstrate the new methodologies in in vivo biological imaging. The proposed research consists of two sequential thrusts: the first involves the development of a novel energetic excitation source for the exploration of the new spectral window between 1600 and 1800 nm (i.e., the 1700-nm spectral window) with significantly reduced tissue scattering; the second thrust concentrates on demonstrating the new methodologies for in vivo deep tissue imaging based on three-photon excitation, improving the signal-to-background ratio by orders of magnitude and extending the depth penetration of multiphoton imaging. The proposed program is based on three major innovations: (1) 1700-nm spectral window for significantly reduced tissue scattering, (2) 3PE as a new excitation modality to simultaneously improve the SBR and extend the accessibility of fluorophores in deep tissue imaging, and (3) an excitation source tailored for in vivo deep tissue three-photon fluorescence microscopy at the 1700-nm spectral window by using soliton self-frequency shift in a photonic crystal rod to generate energetic, wavelength tunable solitons seeded from a fiber laser. We aim to demonstrate a new generation of multiphoton microscopic imaging tool that can reach an ultimate imaging depth of 3 mm or beyond within intact biological tissues such as the mouse or rat brain. The successful completion of this program will have a broad impact on a wide variety of biological and biomedical research fields where high-resolution imaging deep within intact tissue is required. PUBLIC HEALTH RELEVANCE: The proposed program, if successfully completed, leads to a new generation of multiphoton microscopic imaging tool that can reach an ultimate imaging depth of 3 mm or beyond within intact biological tissues such as the mouse or rat brain. The successful completion of this program will have a broad impact on a wide variety of biological and biomedical research fields where high-resolution imaging deep within intact tissue is required.

描述（由申请人提供）：该研究计划的目的是探索新的激光技术，新的光谱窗口和三光子荧光显微镜，以深入散射组织，然后在体内生物学成像中演示新方法。提出的研究由两个顺序推力组成：第一个涉及开发新型的能量激发源，以探索1600 nm之间新的光谱窗口（即1700-nm光谱窗口），并具有显着减少的组织散射；第二个推力集中于基于三光子激发的体内深层组织成像的新方法，从而通过数量级来改善信噪比，并扩展了多光子成像的深度渗透。 The proposed program is based on three major innovations: (1) 1700-nm spectral window for significantly reduced tissue scattering, (2) 3PE as a new excitation modality to simultaneously improve the SBR and extend the accessibility of fluorophores in deep tissue imaging, and (3) an excitation source tailored for in vivo deep tissue three-photon fluorescence microscopy at the 1700-nm spectral window by using soliton光子晶体棒中的自频移位，以产生从纤维激光器种子的能量，波长可调的孤子。我们旨在展示新一代的多光子微观成像工具，该工具可以在完整的生物组织（如小鼠或大鼠脑）内到达3 mm或更远的最终成像深度。该程序的成功完成将对各种生物学和生物医学研究领域产生广泛的影响，在这些研究领域中，需要在完整组织内深处成像。公共卫生相关性：拟议的程序（如果成功完成）会导致新一代的多光子微观成像工具，该工具可以在完整的生物组织（例如小鼠或老鼠脑）内到达3 mm或更远的最终成像深度。该程序的成功完成将对各种生物学和生物医学研究领域产生广泛的影响，在这些研究领域中，需要在完整组织内深处成像。