Optical Instrumentation

光学仪器

• 批准号: 10247576
• 负责人: DAVID W TANK
• 金额: $ 27.79万
• 依托单位: PRINCETON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-28 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10247576
• 关键词: Address; Area; BRAIN initiative; Back; Behavioral; Brain; Calcium; Cerebellum; Collaborations; Corpus striatum structure; Custom; Data; Diffusion; Engineering; Ensure; Goals; Head; Human Resources; Image; Institution; Lasers; Maintenance; Methods; Microscope; Microscopy; Mind; Mus; Neocortex; Neurons; Optics; Photons; Population; Postdoctoral Fellow; Quality Control; Rattus; Refractive Indices; Research Personnel; Research Project Grants; Resolution; Rotation; Science; Short-Term Memory; Standardization; Students; Surface; System; Technology; Time; Transgenic Organisms; Ventral Tegmental Area; Work; base; cell type; cognitive ability; design; design and construction; experimental study; improved; innovation; instrument; instrumentation; lens; multi-photon; neural circuit; neuromechanism; new technology; novel; optogenetics; recruit; relating to nervous system; sample fixation; stereoscopic; success; two-photon; virtual

Project Summary: Core 5, Optical Instrumentation Working memory, the ability to temporarily hold multiple pieces of information in mind for manipulation, is central to virtually all cognitive abilities. This multi-component research project aims to comprehensively dissect the neural circuit mechanisms of this ability across multiple brain areas. Our projects have been designed to take full advantage of new optics-based technologies developed in the BRAIN Initiative. It is imperative that we continue to innovate and upgrade our instrumentation and methods as the cutting edge of the field moves forward. The Optical Instrumentation Core will develop, implement, and support the optical microscopes and other optics-based instrumentation used in our projects to ensure that they represent the best of the new technologies. To do so, we will leverage the technical and engineering expertise in Princeton’s Bezos Center for Neural Circuit Dynamics and recruit additional staff devoted to constructing and maintaining our state-of-the-art instruments. Our projects depend on the latest technology for two- and three-photon calcium imaging at cellular resolution, laser-based optogenetic perturbation systems combined with simultaneous imaging, and novel forms of widefield microscopy. Some of this instrumentation is based on recent innovations in the labs of our PIs. In other cases, we will collaborate with colleagues at other institutions who have developed innovative new methods. Creating an Optical Instrumentation Core will address the problem that much of the technical work required to innovate and maintain these instruments has shifted to students and postdocs, because it has exceeded the capacity of existing staff. This division of labor is a problem for four reasons: (1) lab personnel often do not have sufficient time or expertise to produce the best possible results, (2) the diffusion of responsibility leads people to duplicate one another’s efforts, (3) researchers spend their time on technical work at the expense of doing science, and (4) expertise can be lost as students and postdocs move on. For all these reasons, we propose to standardize this function across projects to improve quality control and efficiency. Centralizing the design, construction, maintenance, and support of these instruments will increase the efficiency and rigor of our microscopy experiments, while freeing lab personnel to focus on designing experiments and collecting data.

项目概要：Core 5，光学仪器 工作记忆是一种在头脑中暂时保存多条信息以进行操作的能力， 对几乎所有认知能力都至关重要这一多方面的研究项目旨在全面 在多个大脑区域中剖析这种能力的神经回路机制。我们的项目已经 旨在充分利用BRAIN Initiative开发的新光学技术。是 我们必须继续创新和升级我们的仪器和方法，作为 磁场向前移动。光学仪器核心将开发，实施和支持光学仪器。 显微镜和其他基于光学的仪器在我们的项目中使用，以确保他们代表 最好的新技术。为此，我们将利用普林斯顿大学的技术和工程专业知识， 贝佐斯神经电路动力学中心，并招募更多的工作人员致力于构建和维护 我们最先进的仪器我们的项目依赖于双光子和三光子的最新技术 细胞分辨率的钙成像，基于激光的光遗传学微扰系统， 同时成像和新形式的宽视场显微镜。其中一些仪器是基于 我们私人侦探实验室的最新创新在其他情况下，我们将与其他同事合作， 开发创新方法的机构。创建光学仪器核心将 解决的问题，许多技术工作所需的创新和维护这些文书， 转移到学生和博士后，因为它已经超过了现有员工的能力。的此划分 劳动力是一个问题，原因有四：（1）实验室人员往往没有足够的时间或专业知识来生产 最好的结果，（2）责任的分散导致人们重复彼此的努力，（3） 研究人员把时间花在技术工作上，而牺牲了科学研究;（4）专业知识可能会流失 由于所有这些原因，我们建议将此功能标准化， 项目，以提高质量控制和效率。集中设计、建造、维护和 这些仪器的支持将提高我们显微镜实验的效率和严谨性， 使实验室人员能够专注于设计实验和收集数据。