3D Bioprinted Omentum tissue to model ovarian cancer metastasis

3D 生物打印网膜组织用于模拟卵巢癌转移

• 批准号: 10255275
• 负责人: Marc Ferrer-Alegre
• 金额: $ 72.57万
• 依托单位: NATIONAL CENTER FOR ADVANCING TRANSLATIONAL SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10255275
• 关键词: 3-Dimensional; Animal Model; Antineoplastic Agents; Biological Assay; Blood Vessels; Cancer Cell Growth; Cell Line; Cell model; Cell-Matrix Junction; Cells; Development; Endothelial Cells; Fibroblasts; Fluorescence Microscopy; Functional disorder; Genetically Engineered Mouse; Goals; Growth; Human; Immunodeficient Mouse; In Vitro; Label; Location; Malignant Neoplasms; Malignant neoplasm of ovary; Mesothelial Cell; Metastatic Malignant Neoplasm to the Ovary; Modeling; Monitor; Morphology; Neoplasm Metastasis; Omentum; Patients; Pericytes; Physiological; Physiology; Preclinical Testing; Production; Protocols documentation; Site; Surface; Suspensions; Three-Dimensional Imaging; Tissues; Toxicity Tests; Xenograft procedure; biofabrication; bioprinting; cancer cell; cell growth; cellular imaging; clinical efficacy; drug development; drug discovery; efficacy testing; monolayer; neoplastic cell; novel therapeutics; ovarian neoplasm; predictive test; prevent; screening; tumor

We have developed biofabrication protocols for the production of vascularized omentum model that includes patient derived fibroblasts, endothelial cells and pericytes, and a layer of patient mesothelial cells. Patient cells were obtained from our collaborators Dr. Ernst Lengyel and Hilary Kenny. Addition of a suspension of fluorescently labeled ovarian tumor cells to the top of mesothelial cell layer on the tissue and used fluorescence microscopy to monitor tumor formation inside the tissue. 3D imaging analysis have been developed to quantiate size and number of tumors, and their location. These analytics will be used to screen a focused set of compounds for compounds that prevent tumor formation in the tissue.

我们已经开发了生产血管化网膜模型的生物制造方案，包括患者来源的成纤维细胞，内皮细胞和周细胞，以及一层患者间皮细胞。患者细胞来自我们的合作者恩斯特·伦格尔博士和希拉里·肯尼。将荧光标记的卵巢肿瘤细胞悬浮液添加到组织间皮细胞层的顶部，并使用荧光显微镜监测组织内肿瘤的形成。三维成像分析已经发展到量化肿瘤的大小和数量，以及它们的位置。这些分析将用于筛选一组集中的化合物，以防止组织中肿瘤形成的化合物。