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3D Bioprinted lung cancer models for drug screening

用于药物筛选的 3D 生物打印肺癌模型

基本信息

批准号：
10255317
负责人：
Marc Ferrer-Alegre
金额：
$ 72.57万
依托单位：
NATIONAL CENTER FOR ADVANCING TRANSLATIONAL SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10255317
关键词：
3-Dimensional Aftercare Animal Model Antineoplastic Agents Biological Assay Bladder Blood Vessels Breast Cancer Cell Growth Cancer Detection Cancer Model Cell Line Cell model Development Differentiation and Growth Disease Drug Modelings Drug Screening Endothelial Cells Epithelial Epithelial Cells Epithelium Fibroblasts Functional disorder Genetically Engineered Mouse Goals Growth Human Immunodeficient Mouse In Vitro Label Lung Malignant Neoplasms Malignant neoplasm of lung Malignant neoplasm of urinary bladder Mesothelial Cell Mesothelioma Modeling Monitor Morphology Neoplasm Metastasis Non-Small-Cell Lung Carcinoma Patients Pericytes Physiological Physiology Pleura Pleural Preclinical Testing Process Proteins Protocols documentation Recurrence Structure of parenchyma of lung Surface Therapeutic Therapeutic Intervention Tissues Toxicity Tests Xenograft procedure airway epithelium angiogenesis biomarker discovery bioprinting cancer cell cell growth cellular imaging clinical efficacy drug development drug discovery efficacy testing lentivirally transduced lung basal segment monolayer neoplastic cell new therapeutic target novel therapeutics origen predictive test prevent screening transcriptomics tumor vasculogenesis

项目摘要

We have established protocols for the bioprinting of a lung vascularized basal tissue using primary lung endothelial cells, pericytes and fibroblasts. IHC and fluroescence miscorpcopy was used to establish vasculogenesis and angiogenesis. We also developed protocols for the labeling of cancer cells with lentivirus transduced florescence proteins and monitor tumor formation in the tissue for a set of bladder cancer cells with different metastatic origen. We are also establishing a model of the lung pleural by layering primary mesothelial cells on top of the vascularized lung base tissue to create model of mesothelioma in a pleura tissue. We are also establishing protocol to layer and differentiate small airway epithelial cells (SAEC) on to create model of NSCLC. Currently, we have established protocols for SAEC growth and differentiation and we are in the process of developing protocols to assemble the complete small airway lung tissue.

我们已经建立了使用原代肺内皮细胞、周细胞和成纤维细胞进行肺血管化基底组织生物打印的方案。采用免疫组化和荧光错影法建立血管新生和血管新生。我们还开发了用慢病毒转导的荧光蛋白标记癌细胞的方案，并监测组织中具有不同转移源的一组膀胱癌细胞的肿瘤形成。我们还建立了一个肺胸膜模型，通过将原发性间皮瘤细胞分层在血管化的肺基底组织上，以创建胸膜组织中的间皮瘤模型。我们也正在建立小气道上皮细胞（SAEC）分层和分化的方案，以建立非小细胞肺癌模型。目前，我们已经建立了SAEC生长和分化的方案，我们正在制定组装完整的小气道肺组织的方案。