3D Bioprinted lung cancer models for drug screening

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We have established protocols for the bioprinting of a lung vascularized basal tissue using primary lung endothelial cells, pericytes and fibroblasts. IHC and fluroescence miscorpcopy was used to establish vasculogenesis and angiogenesis. We also developed protocols for the labeling of cancer cells with lentivirus transduced florescence proteins and monitor tumor formation in the tissue for a set of bladder cancer cells with different metastatic origen. We are also establishing a model of the lung pleural by layering primary mesothelial cells on top of the vascularized lung base tissue to create model of mesothelioma in a pleura tissue. We are also establishing protocol to layer and differentiate small airway epithelial cells (SAEC) on to create model of NSCLC. Currently, we have established protocols for SAEC growth and differentiation and we are in the process of developing protocols to assemble the complete small airway lung tissue.