HIV-1 Genomic RNA Integrity
HIV-1 基因组 RNA 完整性
基本信息
- 批准号:10241263
- 负责人:
- 金额:$ 23.4万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2020
- 资助国家:美国
- 起止时间:2020-08-18 至 2023-07-31
- 项目状态:已结题
- 来源:
- 关键词:Applications GrantsAttenuatedBioinformaticsBiologicalBiological AssayCRISPR interferenceCRISPR libraryCRISPR screenCandidate Disease GeneCellsComplexEnzymesExploratory/Developmental GrantGenesGeneticGenetic MaterialsGenetic ScreeningGenomeGenomicsGoalsHIV GenomeHIV-1High-Throughput Nucleotide SequencingInformaticsLeadLentivirus VectorLibrariesLightLogicMediatingMethodologyMethodsMolecular ProfilingMonitorNorthern BlottingNucleic AcidsPathway interactionsPhenotypePopulationPreparationPrimatesProceduresPropertyQuality ControlRNARNA ProcessingRNA VirusesRNA-Directed DNA PolymeraseRecordsReproducibilityResearchRetroviridaeReverse Transcriptase Polymerase Chain ReactionReverse TranscriptionRunningStructureTestingTimeVirionVirusVirus DiseasesVirus ReplicationWorkbasedesigndimerexperienceexperimental studygenome integritygenomic RNAinnovative technologiesinsightmindfulnessmonomermutantnovelnovel strategiessuccesstechnology developmentvectorviral genomicswhole genome
项目摘要
HIV-1 genomic RNA integrity
Retroviruses are the only viruses that encapsidate two copies of their genetic material. One possible reason for
this is that retroviral RNA appears to be damaged prior to reverse transcription; therefore, the presence of two
copies of viral genomic RNA allows for successful reverse transcription through template switching. When
virion RNA is examined on non-denaturing northern blots, it runs as a single dimer band. However, when heat-
denatured to disrupt the dimer linkage, virion RNA includes a monomer band of reduced intensity compared to
the dimer and also a smear of more rapidly-migrating RNAs, suggesting encapsidated RNA is extensively
fragmented. Our central hypothesis is that this fragmentation of HIV-1 genomic RNA is due to the action of
one or more host enzymes, the absence of which would lead to virions with more intact genomic RNAs and
higher infectious titers. The objective of this proposal is to identify cellular genes that are associated with HIV-
1 RNA nicking using unbiased genetic screens and to test if these represent a new class of restriction factors.
The proposed work combines Telesnitsky lab expertise in retroviral RNA with Tai lab expertise in whole-
genome genetic screens for cellular factors that modulate viral infection plus Kidd lab expertise in specialized
library preparation and bioinformatics, and consists of two aims: In Aim 1: we will seek to identify cellular genes
that are required for observed damage to encapsidated HIV-1 RNAs using a whole-genome CRISPR library
encoded in a lentiviral vector, and will validate candidate genes by using genetic depletion and rescue
experiments. In Aim 2: we will assess properties of virion RNA fragmentation and examine its ramifications for
viral replication. If successful, this project may reveal a new class of cellular restriction factors, may help
illuminate novel cellular RNA processing pathways if the identified genes have not previously been shown to
participate in RNA quality control, and may provide new insight into virion RNA structures and accessibility.
HIV-1基因组RNA完整性
项目成果
期刊论文数量(2)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Directed evolution of potent neutralizing nanobodies against SARS-CoV-2 using CDR-swapping mutagenesis.
- DOI:10.1016/j.chembiol.2021.05.019
- 发表时间:2021-09-16
- 期刊:
- 影响因子:8.6
- 作者:Zupancic JM;Desai AA;Schardt JS;Pornnoppadol G;Makowski EK;Smith MD;Kennedy AA;Garcia de Mattos Barbosa M;Cascalho M;Lanigan TM;Tai AW;Tessier PM
- 通讯作者:Tessier PM
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Jeffrey M Kidd其他文献
Genome sequencing and analysis of admixed genomes of African and Mexican ancestry: implications for personal ancestry reconstruction and multi-ethnic medical genomics
非洲和墨西哥血统混合基因组的基因组测序与分析:对个人血统重建和多族裔医学基因组学的意义
- DOI:
10.1186/gb-2010-11-s1-o4 - 发表时间:
2010-10-11 - 期刊:
- 影响因子:9.400
- 作者:
Francisco M De La Vega;Katarzyna Bryc;Jeremiah D Degehnardt;Shaila Musharoff;Jeffrey M Kidd;Vrunda Seth;Sarah Stanley;Abra Brisbin;Alon Keinan;Andrew Clark;Carlos D Bustamante - 通讯作者:
Carlos D Bustamante
Jeffrey M Kidd的其他文献
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{{ truncateString('Jeffrey M Kidd', 18)}}的其他基金
Identification of Factors Critical for SINE Retrotransposition
确定 SINE 逆转位的关键因素
- 批准号:
10311056 - 财政年份:2020
- 资助金额:
$ 23.4万 - 项目类别:
Identification of Factors Critical for SINE Retrotransposition
确定 SINE 逆转位的关键因素
- 批准号:
10095880 - 财政年份:2020
- 资助金额:
$ 23.4万 - 项目类别:
Identification of Factors Critical for SINE Retrotransposition
确定 SINE 逆转位的关键因素
- 批准号:
10527360 - 财政年份:2020
- 资助金额:
$ 23.4万 - 项目类别:
Characterizing the Global Architecture of Genomic Diversity
描述基因组多样性的全球结构
- 批准号:
8537229 - 财政年份:2011
- 资助金额:
$ 23.4万 - 项目类别:
Characterizing the Global Architecture of Genomic Diversity
描述基因组多样性的全球结构
- 批准号:
8413227 - 财政年份:2011
- 资助金额:
$ 23.4万 - 项目类别:
Characterizing the Global Architecture of Genomic Diversity
描述基因组多样性的全球结构
- 批准号:
8915986 - 财政年份:2011
- 资助金额:
$ 23.4万 - 项目类别:
Characterizing the Global Architecture of Genomic Diversity
描述基因组多样性的全球结构
- 批准号:
8213131 - 财政年份:2011
- 资助金额:
$ 23.4万 - 项目类别:
Characterizing the Global Architecture of Genomic Diversity
描述基因组多样性的全球结构
- 批准号:
8715423 - 财政年份:2011
- 资助金额:
$ 23.4万 - 项目类别:
Characterizing the Global Architecture of Genomic Diversity
描述基因组多样性的全球结构
- 批准号:
8335440 - 财政年份:2011
- 资助金额:
$ 23.4万 - 项目类别:
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