Gastrointestinal Biology Core
胃肠生物学核心
基本信息
- 批准号:10241580
- 负责人:
- 金额:$ 21.97万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2018
- 资助国家:美国
- 起止时间:2018-07-01 至 2025-06-30
- 项目状态:未结题
- 来源:
- 关键词:16S ribosomal RNA sequencing3-DimensionalAffectAnimal ExperimentationAspirate substanceBacteriaBasic ScienceBioinformaticsBiologicalBiological AssayBiological ModelsBiological Response Modifier TherapyBiologyBiometryBiopsyBiostatistics CoreCell Culture TechniquesCell LineCell modelCellsCellular biologyChildClinicalClinical ResearchCoculture TechniquesCollaborationsCystic FibrosisCystic Fibrosis Transmembrane Conductance RegulatorDataDevelopmentDoctor of PhilosophyEcologyEducational workshopElectrophysiology (science)EngineeringFacultyFecesForskolinFosteringGastrointestinal DiseasesGenomicsGillsGoalsGram-Negative BacteriaHumanImmunologyIn VitroIndustryInnate Immune ResponseIntestinesIonsKidneyLongevityMethodologyMethodsMicrobeMicrobiologyMissionModelingMolecularMolecular BiologyMusMutationOrganoidsPermeabilityPharmaceutical PreparationsPhysiologyPilot ProjectsProbioticsPublishingQuantitative Reverse Transcriptase PCRReagentResearchResearch PersonnelResistanceScienceScientistServicesSwellingSystemTechniquesTestingTherapeuticTissue SampleTissuesTrainingTraining SupportTransfectionTranslational ResearchUniversitiesWorkcystic fibrosis mousecystic fibrosis patientsexperimental studygastrointestinalgastrointestinal bacteriagastrointestinal epitheliumgut dysbiosisgut microbiomeindustry partnermembermetabolomicsmicrobialmicrobiomemonolayermouse modelnovelnovel therapeuticsorgan on a chippathogenpersonalized medicineprofessorprogramsprotein transportsingle-cell RNA sequencingsystemic inflammatory responsetooltool developmenttranscriptome sequencing
项目摘要
The mission of the Gastrointestinal Biology Core (GIBC) is to advance basic science, as well as translational
and discovery studies relevant to gastrointestinal (GI) disease in CF. A central service of this Core is to offer a
broad spectrum of model systems, expertise in the selection of models and analytical approaches, and the
introduction or development of new model systems to facilitate GI research by DartCF scientists. The GIBC
has implemented a suite of models to study GI disease in CF, including paired WT and CF mouse and human
3D intestinal organoids, 2D monolayers derived from intestinal organoids, Gut-On-A-Chip systems, engineered
cell lines, transformation reagents and mouse models. The human GI cell models have been developed in
collaboration with the Clinical Research Translation Core (CRTC), which provided biopsy tissue samples and
GI clinical bacterial isolates. The GIBC will facilitate ongoing studies of DartCF investigators, our collaborators
at other CF Research Centers, and pilot studies supported by the DartCF Pilot Project Program. The Core will
also assist in the onboarding or development of novel methodologies, as needed, to advance the science of
Center members and Pilot Project awardees. Notable services of the GIBC include: (1) Support for GI host-
microbe co-culture studies. In the first year of support the GIBC has implemented 3D organoids and 2D
organoid-derived monolayers from mice and humans, leveraged its expertise in co-culture models to study
interactions between these models and GI bacteria, initiated the Gut-On-A-Chip model, and characterized over
30 bacterial isolates obtained from the stool of CF and non-CF children and from endoscopic aspirates
(CTRC); (2) Expertise in single cell-RNA-sequencing, other molecular techniques, and characterization of GI
organoids, working with the CF Bioinformatics and Biostatistics Core (CF-BBC); (3) Support for functional
studies including forskolin-induced swelling assays (FIS) of 3D GI organoids, and electrophysiological studies
of 2D organoid monolayers. In the first year of support we performed FIS assays on mouse and human 3D
colonoids, and Ussing chamber experiments on 2D organoid monolayers; (4) Support for studies on mouse
models to study GI disease in CF; (5) Coordination with industry to develop new multi-organ therapeutics
relevant to CF, and (6) Training in the use of GI microbe-host co-culture organoid models and mouse models
to members of the Center as well as investigators regionally, nationally, and around the world. The GIBC
launched in 2018 to meet the research needs of DartCF faculty, and to facilitate GI research at Dartmouth.
This Core is co-directed by Bruce Stanton, PhD, the Andrew C. Vail Professor of Microbiology and Immunology
and Deborah Hogan, PhD, Professor of Microbiology and Immunology. Dr. Stanton and Dr. Hogan have
complementary expertise covering CF, kidney physiology, cell and molecular biology, protein trafficking,
microbiology, microbial ecology, host-pathogen and polymicrobial interactions, animal research, and cell
culture, which has fostered the development of the central capabilities of this Core.
胃肠道生物学核心(GIBC)的使命是推动基础科学和翻译
和发现与胃肠道(GI)疾病相关的研究。此核心的一项核心服务是提供
广泛的模型系统,选择模型和分析方法的专门知识,以及
引进或开发新的模型系统,以促进DartCF科学家进行GI研究。GIBC
已经实施了一套模型来研究CF的胃肠道疾病,包括配对的WT和CF小鼠和人
3D肠道器官,源自肠道器官的2D单层,芯片上肠道系统,工程设计
细胞系、转化试剂和小鼠模型。人类胃肠道细胞模型已经在
与临床研究翻译核心(CRTC)合作,CRTC提供活检组织样本和
胃肠道临床分离的细菌。GIBC将促进我们的合作者DartCF调查人员正在进行的研究
在其他CF研究中心,以及DartCF试点项目计划支持的试点研究。核心意志
还根据需要协助入职或开发新的方法,以促进
中心成员和试点项目获奖者。GIBC值得注意的服务包括:(1)支持GI主机-
微生物共培养研究。在支持的第一年,GIBC实施了3D有机物和2D
来自小鼠和人类的有机衍生单层,利用其在共培养模型中的专业知识来研究
这些模型与胃肠道细菌之间的相互作用,启动了芯片上的肠道模型,并在
从CF型和非CF型儿童粪便和胃镜下抽吸物中分离到30株细菌
(2)在单细胞RNA测序、其他分子技术和胃肠道疾病特征方面的专业知识
有机物,与CF生物信息学和生物统计核心(CF-BBC)合作;(3)支持功能
研究包括Forskolin诱导的3D GI有机化合物的肿胀试验(FIS)和电生理研究
二维单分子有机薄膜。在支持的第一年,我们对小鼠和人类3D进行了FIS测试
在二维有机类单分子膜上的Ussing小室实验;(4)支持对小鼠的研究
(5)与产业界合作开发新的多器官疗法
(6)使用GI微生物-宿主共培养器官模型和小鼠模型的培训
该中心的成员以及区域、国家和世界各地的调查人员。GIBC
于2018年推出,以满足DartCF教职员工的研究需求,并促进达特茅斯大学的GI研究。
这一核心由布鲁斯·斯坦顿博士、安德鲁·C·维尔微生物学和免疫学教授共同执导
黛博拉·霍根博士,微生物学和免疫学教授。斯坦顿博士和霍根博士
补充性专业知识涵盖慢性肾功能衰竭、肾脏生理学、细胞和分子生物学、蛋白质运输、
微生物学、微生物生态学、宿主-病原体和多微生物相互作用、动物研究和细胞
文化,这促进了这一核心核心能力的发展。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Bruce A. Stanton其他文献
New insights into cystic fibrosis: molecular switches that regulate CFTR
囊性纤维化的新见解:调节 CFTR 的分子开关
- DOI:
10.1038/nrm1949 - 发表时间:
2006-06-01 - 期刊:
- 影响因子:90.200
- 作者:
William B. Guggino;Bruce A. Stanton - 通讯作者:
Bruce A. Stanton
Characterization of apical and basolateral membrane conductances of rat inner medullary collecting duct.
大鼠内髓集合管顶膜和基底外侧膜电导的表征。
- DOI:
- 发表时间:
1989 - 期刊:
- 影响因子:0
- 作者:
Bruce A. Stanton - 通讯作者:
Bruce A. Stanton
Lung-kidney axis in cystic fibrosis: Early urinary markers of kidney injury correlate with neutrophil activation and worse lung function
囊性纤维化中的肺-肾轴:肾脏损伤的早期尿液标志物与中性粒细胞活化和更差的肺功能相关
- DOI:
10.1016/j.jcf.2024.12.007 - 发表时间:
2025-05-01 - 期刊:
- 影响因子:6.000
- 作者:
Grace M. Rosner;Himanshu B. Goswami;Katherine Sessions;Lindsay K. Mendyka;Brenna Kerin;Irma Vlasac;Diane Mellinger;Lorraine Gwilt;Thomas H. Hampton;Martha Graber;Alix Ashare;William T. Harris;Brock Christensen;Bruce A. Stanton;Agnieszka Swiatecka-Urban;Sladjana Skopelja-Gardner - 通讯作者:
Sladjana Skopelja-Gardner
Bruce A. Stanton的其他文献
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{{ truncateString('Bruce A. Stanton', 18)}}的其他基金
Let-7b in Extracellular Vesicles Secreted by Bronchial Epithelial Cells Increases the Antibiotic Sensitivity of Pseudomonas
支气管上皮细胞分泌的胞外囊泡中的 Let-7b 增加假单胞菌的抗生素敏感性
- 批准号:
10319005 - 财政年份:2020
- 资助金额:
$ 21.97万 - 项目类别:
Let-7b in Extracellular Vesicles Secreted by Bronchial Epithelial Cells Increases the Antibiotic Sensitivity of Pseudomonas
支气管上皮细胞分泌的胞外囊泡中的 Let-7b 增加假单胞菌的抗生素敏感性
- 批准号:
10525239 - 财政年份:2020
- 资助金额:
$ 21.97万 - 项目类别:
Retrieval, Reprocessing, Normalization and Sharing of Gene Expression and Lung Microbiome Data Sets to Facilitate AI/ML Analysis Studies of Bacterial Lung Infections
基因表达和肺部微生物组数据集的检索、再处理、标准化和共享,以促进细菌肺部感染的 AI/ML 分析研究
- 批准号:
10594180 - 财政年份:2020
- 资助金额:
$ 21.97万 - 项目类别:
Dartmouth Lung Biology Center for Molecular, Cellular and Translational Research
达特茅斯肺生物学分子、细胞和转化研究中心
- 批准号:
9115188 - 财政年份:2013
- 资助金额:
$ 21.97万 - 项目类别:
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