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Organ Specific Project - Bone Marrow

器官特定项目 - 骨髓

基本信息

批准号：
10251351
负责人：
KATHRIN M BERNT
金额：
$ 44.04万
依托单位：
CHILDREN'S HOSP OF PHILADELPHIA
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-09-01 至 2022-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10251351
关键词：
3-Dimensional Address Adipocytes Aging Anatomy Archives Autopsy Biological Assay Biology Blood Cells Blood Vessels Bone Banks Bone Growth Bone Marrow Bone Marrow Cells Bone Tissue Cell Nucleus Cells Cellular Assay Chromatin Clinical Collection Communities Complex Consent Data Detection Development Disease Doctor of Philosophy Endothelium Epidemiology Epigenetic Process Extracellular Matrix Female Fluorescent in Situ Hybridization Future Genetic Transcription Genomics Goals Heart Hematopoiesis Hematopoietic Hematopoietic stem cells Histologic Homeostasis Human Human BioMolecular Atlas Program Image Infrastructure Knowledge Leadership Location Longevity Lymphoid Mesenchymal Mesenchymal Stem Cells Metadata Minerals Multiomic Data Myelogenous Organ Osteoblasts Osteocytes Osteogenesis Persons Pilot Projects Play Proteomics Protocols documentation Regulator Genes Research Resolution Role Sampling Series Signal Pathway Small Nuclear RNA Specimen Sternum Stromal Cells System Three-Dimensional Imaging Tissues Transposase age group base biobank bone bone marrow mesenchymal stem cell cell type cellular imaging design epigenome experience indexing inter-individual variation male mechanical properties member microCT multiple omics progenitor programs serial imaging transcriptome transcriptome sequencing transcriptomics

项目摘要

PROJECT SUMMARY – BONE ORGAN SPECIFIC PROJECT The bone is a highly complex organ made of three tissue types with distinct functions, including cells from hematopoietic, mesenchymal and endothelial lineages. Confined within one organ and thoroughly mixed with each other, these three tissues are mutually dependent for their development and homeostasis. The spatial organization of the various cell types and their cross-talk in the bone is a major gap of our knowledge. The goal of the bone Organ Specific Project (B-OSP) is to address this knowledge gap and generate high quality, single- cell resolution, longitudinal imaging and multi-omics data of normal bones across the human lifespan. We propose the following three specific aims: 1) To refine protocols for biospecimen processing, multi-omics and imaging assays and define inter-individual variability using our existing banked bone samples. Using existing biobanked normal bones from the autopsy program, we will perform a series of pilot studies to refine our protocols for biospecimen collection and processing, as well as single-cell omics and imaging assays. 2) To procure, archive, and annotate high-quality normal bone samples across human life span. We have established a streamlined procurement and biorepository infrastructure to support our bone OSP. We will procure normal bone from the same donor of 6 age groups across the human life span. 3) To spatially profile bone specimens across the human life span using a set of robust and scalable imaging and single-cell omics assays. We will use micro computed tomography to image every bone we collect at both the macroscale and mesoscale. At the microscale, we will perform single-nucleus RNA sequencing (snRNA-Seq) and single-cell assay for transposase-accessible chromatin using sequencing (scATAC-Seq) of two strategic anatomical sites per bone to determine cell type composition, as well as transcriptional and epigenetic signatures. We will perform multiplexed error-robust fluorescence in situ hybridization (MERFISH, for transcriptomics) and Co- Detection by Indexing (CODEX, for proteomics) analyses.

项目摘要-骨器官专项项目