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Center for Developmental Mapping of Heart and Bone Tissues

心脏和骨组织发育图谱中心

基本信息

批准号：
10118850
负责人：
KATHRIN M BERNT
金额：
$ 50万
依托单位：
CHILDREN'S HOSP OF PHILADELPHIA
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-09-01 至 2022-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10118850
关键词：
Adult Age Aging Algorithms Anatomy Atlases Automobile Driving Awareness Basic Science Biological Assay Biology Biomedical Research Bone Diseases Bone Tissue Boston Cell Communication Cell Nucleus Cells Cellular Assay Cellular Structures Chromatin Clinical Data Clinical Research Collaborations Communication Communities Complex Computational algorithm Computer Models Computer software Computing Methodologies Data Databases Detection Development Diagnosis Disease Emerging Technologies Ethnic Origin Fluorescent in Situ Hybridization Follow-Up Studies Freezing Genetic Transcription Health Heart Heart Diseases Hematological Disease Heterogeneity Human Human BioMolecular Atlas Program Image Imaging technology Informed Consent Infrastructure Iowa Joints Knowledge Longevity Maps Mediating Medical Methods Molecular Molecular Profiling Normalcy Organ Outcome Pathogenesis Pattern Pediatric Hospitals Pennsylvania Phenotype Philadelphia Physiological Play Population Proteins Protocols documentation RNA Regulatory Pathway Research Research Personnel Role Signal Pathway Signal Transduction Pathway Small Nuclear RNA Spatial Distribution Specimen Techniques Technology Tissue Donors Tissue Preservation Tissue Procurements Tissues Translational Research Transposase Universities Visualization base biobank bone bone quality burden of illness cell type cellular imaging complex data computational pipelines computerized tools data sharing epidemiologic data genomic data human disease human tissue improved indexing infancy insight molecular imaging multiple omics multiplexed imaging novel open source outcome forecast precision medicine sex single cell analysis transcriptome sequencing user-friendly

项目摘要

PROJECT SUMMARY - OVERALL Both human bone and heart present remarkable anatomical, cellular and functional heterogeneity, with specialized cellular structures performing distinct yet essential physiological functions. A significant gap of knowledge is that different cells' molecular signature, spatial distribution and interactions, and functional state remain little understood for both organs. Although spatially separated, growing evidence suggests that a bone- heart communication axis plays a critical role in normal development and pathogenesis of these two organs and possibly other organs. Joint analysis of the two organs could reveal novel insights into the microenvironment organization and signaling pathways underlying the inter-organ communication. We therefore propose the in-depth characterization of these two organs which, together, account for a large fraction of human disease burden. We will map molecular and cellular changes in the tissue over the course of human lifespan using comprehensive multi-dimensional single-cell and imaging technologies. The final product will impact research of these two organs in the following manner: 1) Organ Atlases: spatially resolved atlases will provide a highly user friendly, publicly available, searchable database of the most comprehensive multi- omic, single cell analysis of the two organs. Molecular data will be richly annotated with additional clinical and epidemiological data. 2) Computational methods: in addition to the data, the critical computational tools and pipelines developed in this project will be available to the research community. These include methods and pipelines for processing multi-omics and imaging data, inference of cell-specific regulatory and signaling pathways, correlation of mesoscale imaging and molecular imaging features, as well as database algorithms for the query, exploration and visualization of highly complex data. 3) Access to biospecimens for follow-up studies: biospecimens collected in this project will be banked and made available to the biomedical research community. These include freshly frozen and fixed specimens and tissue sections. In summary, the proposed project will broadly impact the entire research community and jumpstart basic-science and medical discoveries based on a sophisticated understanding of the key molecular circuits underlying the development and aging of these two organs.

项目总结-整体