Core B - Resource for Pre-Clinical Studies of AKI

项目摘要 人类疾病的小动物模型为研究肾脏病理生理学提供了重要的见解。 过程，是测试治疗和预防方法的一个重要的临床前资源 肾损伤(AKI)。核心B的具体目标是提供学习所需的设施和技能 小鼠AKI模型，2)小动物显像，3)确定肾脏病理生理变化 阿琪。这一核心将具体提供(1)在使用啮齿动物模型方面的开发和培训方面的专门知识 AKI专门在设置缺血/再灌注损伤、脓毒症和肾移植时，(Ii)一种多- 形态小动物成像核心，将提供最先进的分子成像，包括功能， 使用磁共振成像/光谱学的结构和代谢成像，高频 超声、微型CT、伽马射线成像(伽马相机、微型SPECT/CT、微型PET/CT)和光学 成像(生物发光和荧光)；以及(3)将提供专门知识和培训的生理学核心 用于研究全肾和单个肾单位水平的肾功能，包括显微穿刺术 肾小球滤过率的技术和测定、肾小管液和肾小管重吸收的微量分析 评估肾小管球反馈的血流动力学，以及评估肾脏氧气的代谢 啮齿动物的消耗量。核心B还将提供初级管材和管材隔离的技术专长 啮齿动物的血管细胞。 核心B的目的是提供独特的资源，帮助调查人员克服障碍以利用 用于体内研究的相关啮齿动物模型和用于体外研究的啮齿动物肾脏细胞和细胞系进展 对AKI的病理生理学的认识。核心B在支持肾脏方面非常成功 研究社区。自奥布莱恩中心成立以来，Core B已经演出了超过12,000场 200名调查人员的程序。每年都有稳定数量的调查人员使用Core B。在现在 在供资周期内，在120名调查员中，91名(76%)为非核心调查员。核心B也支持 20名试点和可行性及催化剂奖获得者的研究成果。这些综合努力一直是 目前在105份同行评议的出版物中得到认可。复杂的基础设施与 Core B的独特专业知识将继续促进我们的研究人员基础和 AKI研究进展。

Project Summary Small animal models of human disease have provided important insights into renal pathophysiological processes and are an important preclinical resource to test therapeutic and preventive approaches in acute kidney injury (AKI). The specific aims of Core B are to provide the facilities and requisite skills 1) to study murine models of AKI, 2) for small animal imaging, and 3) to determine renal pathophysiological changes in AKI. This core will specifically provide (i) expertise in development and training in the use of rodent models of AKI specifically in the setting of ischemia/reperfusion injury, sepsis and kidney transplantation, (ii) a multi- modality small animal imaging core that will provide state-of-the-art molecular imaging, including functional, structural and metabolic imaging using magnetic resonance imaging/spectroscopy, high frequency ultrasonography, microCT, gamma-ray imaging (gamma camera, microSPECT/CT, microPET/CT), and optical imaging (bioluminescence and fluorescence), and (iii) a physiology core that will provide expertise and training for studying renal function on the whole kidney and at the single nephron level, including micropuncture techniques and determination of GFR, microanalysis of tubular fluid and tubular reabsorption, renal hemodynamics with assessment of tubuloglomerular feedback, and metabolic assessment of kidney oxygen consumption in rodents. Core B will also provide technical expertise for the isolation of primary tubular and vascular cells from rodents. The intent of Core B is to provide unique resources that help overcome barriers for investigators to utilize relevant rodent models for in vivo studies and rodent kidney cells and cell lines for in vitro studies to advance understanding of the pathophysiology of AKI. Core B has been very successful in supporting the kidney research community. Since the inception of the O'Brien Center, Core B has performed more than 12,000 procedures for 200 investigators. A steady number of investigators use Core B each year. In the present funding cycle, of the 120 investigators, 91 (76%) were non-core investigators. Core B has also supported the research efforts of 20 Pilot and Feasibility and Catalyst grant awardees. These combined efforts have been currently recognized in 105 peer-reviewed publications. The sophisticated infrastructure coupled with the unique expertise of Core B will continue to catalyze collaborative activities of our investigator base and advances in AKI research.