Resource for Pre-Clinical Studies of AKI (Animal Models/lmaging/Renal Physiology

人类疾病的小鼠模型为肾脏病理生理过程提供了重要的见解，并且是测试急性肾损伤（阿基）治疗和预防方法的重要临床前资源。核心B的具体目的是提供设施和必要技能，1）研究阿基的鼠模型，2）用于小动物成像，和3）确定阿基中的肾脏生理变化。该核心将专门提供（i）在缺血/再灌注损伤、脓毒症和肾移植背景下使用阿基啮齿动物模型的开发和培训方面的专业知识，（ii）多模态小动物成像核心，将提供最先进的分子成像，包括使用磁共振成像/光谱学、高频超声检查、microCT、伽马射线成像（伽马照相机、microSPECT/CT、microPET/CT）和光学成像（生物发光和荧光），和（iii）生理学核心，将提供专门知识和培训，用于研究整个肾脏和单个肾单位水平的肾功能，包括显微穿刺技术和GFR测定，小管液和小管重吸收的微量分析，肾血流动力学与肾小管肾小球反馈的评估，以及啮齿动物肾脏耗氧量的代谢评估。核心B还将提供从啮齿动物培养物中分离原代肾细胞和血管细胞的技术专长。 核心B的目的是提供独特的资源，帮助研究者克服障碍，利用相关啮齿动物模型进行体内研究，利用啮齿动物细胞系进行体外研究，以促进对阿基病理生理学的理解。核心B在支持肾脏研究界方面非常成功。核心B自启动以来，已为80名主要研究者执行了5,800多项程序，涉及92个项目。在80名调查员中，60名（75%）为非核心人员。每年使用核心B的研究者人数正在增加，年度出版率也在增加。核心项目B还支持了11个试点和可行性赠款获得者的研究工作。这些综合努力目前已在83份同行审查的出版物中得到承认。先进的基础设施加上核心B的独特专业知识将继续促进UCSD和UCSD之间的合作，并产生创新的新举措，推动阿基研究。

Mouse models of human disease have provided essential insights into renal pathophysiological processes and are an important preclinical resource to test therapeutic and preventive approaches in acute kidney injury (AKI). The specific aims of Core B are to provide the facilities and requisite skills 1) to study murine models of AKI, 2) for small animal imaging, and 3) to determine renal physiological changes in AKI. This core will specifically provide (i) expertise in development and training in the use of rodent models of AKI specifically in the setting of ischemia/reperfusion injury, sepsis and renal transplantation, (ii) a multi-modality small animal imaging core that will provide state-of-the-art molecular imaging, including functional, structural and metabolic imaging using magnetic resonance imaging/spectroscopy, high frequency ultrasonography, microCT, gamma-ray imaging (gamma camera, microSPECT/CT, microPET/CT), and optical imaging (bioluminescence and fluorescence), and (iii) a physiology core that will provide expertise and training for studying renal function on the whole kidney and at the single nephron level, including micropuncture techniques and determination of GFR, microanalysis of tubular fluid and tubular reabsorption, renal hemodynamics with assessment of tubuloglomerular feedback, and metabolic assessment of kidney oxygen consumption in rodents. Core B will also provide technical expertise for the isolation of primary renal and vascular cells in culture from rodents. The intent of Core B is to provide unique resources that help overcome barriers for investigators to utilize relevant rodent models for in vivo studies and rodent cell lines for in vitro studies to advance understanding of the pathophysiology of AKI. Core B has been very successful in supporting the kidney research community. Since the inception of Core B, more than 5,800 procedures have been performed for 80 principal investigators involving 92 projects. Of the 80 investigators, 60 (75%) were non-core personnel. The number of investigators using Core B each year is increasing, as is the annual publication rate. Core B has also supported the research efforts of 11 Pilot and Feasibility grant awardees. These combined efforts have been currently recognized in 83 peer-reviewed publications. The sophisticated infrastructure coupled with the unique expertise of Core B will continue to catalyze collaborative efforts between and UCSD and produce innovative new initiatives that will advance AKI research.