Touchscreen-compatible, real-time electrochemical sensing of SARS-CoV-2

触摸屏兼容的 SARS-CoV-2 实时电化学传感

• 批准号: 10260946
• 负责人: Suzie H. Pun
• 金额: $ 46.65万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-12-21 至 2022-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10260946
• 关键词: 2019-nCoV; Affinity; Air; Antibodies; Binding; Binding Proteins; Biosensing Techniques; Biosensor; COVID-19; COVID-19 detection; COVID-19 pandemic; COVID-19 patient; Car Phone; Cellular Phone; Cessation of life; Chemistry; Clinical; Contact Tracing; Coronavirus; Detection; Development; Devices; Disease Outbreaks; Early Diagnosis; Electric Capacitance; Electrodes; Electronics; Engineering; Evaluation; Exhibits; Face; Frequencies; Future; Glass; Goals; Hospitals; Immobilization; Individual; Infection; Label; Libraries; Measures; Middle East Respiratory Syndrome; Molecular Conformation; Natural regeneration; Oxidation-Reduction; Pathogen detection; Pattern; Performance; Preparation; Process; Reporting; Restaurants; SARS coronavirus; SARS-CoV-2 spike protein; SARS-CoV-2 transmission; Sampling; Sensitivity and Specificity; Signal Transduction; Specificity; Surface; System; Technology; Testing; Time; Touch sensation; Vaccines; Validation; Virion; Work; aptamer; base; cost; density; design; infection rate; instrumentation; nanofabrication; new technology; novel; prevent; secondary infection; sensor; touchscreen; transmission process; viral detection; viral transmission

PROJECT SUMMARY The SARS-CoV-2 coronavirus, the cause of the COVID-19 global pandemic, is efficiently spread and has reached over 27 million confirmed cases as of September 8, 2020. There is therefore an urgent need for new technologies that can provide early detection of virus, reducing the transmission and infection rate. The goal of this proposal is to develop an integrated biosensor- touchscreen that sensitivity reports surface contact with SARS-CoV-2. In our preliminary work, we have identified several aptamers that bind specifically to the envelope-anchored trimeric spike (S) protein of SARS-CoV-2, but not of SARS-CoV or MERS. In comparison to antibodies, aptamers are synthetic molecules that more thermally stable and lower cost while providing similar specificity and affinity of target binding. In this application, we propose to integrate aptamer-based biosensing of SARS-CoV-2 into a touchscreen device. Our main objectives are to 1) engineer conformation switching aptamers for electrochemical sensing of SARS-CoV-2 binding, 2) develop nanogap capacitive sensors as a uniquely complementary approach to capacitive touchscreen technology and 3) build and test an integrated biosensor and touchscreen array that can detect SARS-CoV-2 from patient samples. Successful completion of these aims will result in a novel automatic sensing platform for SARS-CoV-2. This technology could transform personal device touchscreens as well as to multi-user touchscreen devices in hospitals, airports, libraries, restaurants, for early detection, curbing transmission rates from secondary exposure. Importantly, the developed technology could be adapted for other electronic sensing platforms, and easily applied for future pathogen detection.

项目摘要 SARS-COV-2冠状病毒是Covid-19全球大流行的原因，有效地传播 截至2020年9月8日，已经达到了超过2700万案件。 迫切需要新技术，这些技术可以尽早发现病毒，从而减少 传播和感染率。该提案的目的是开发一个综合的生物传感器 - 灵敏度报告与SARS-COV-2的表面接触的触摸屏。在我们的初步工作中 我们已经确定了几个特异性结合的适体 （S）SARS-COV-2的蛋白质，但不是SARS-COV或MERS的蛋白质。与抗体相比 适体是合成分子，在提供的同时更稳定，成本更低 目标结合的类似特异性和亲和力。在此应用程序中，我们建议整合 SARS-COV-2基于适体的生物传感到触摸屏设备。我们的主要目标是 1）工程师构象切换适体用于SARS-COV-2的电化学感测 绑定，2）开发纳米纳法仪电容传感器作为一种独特的互补方法 电容式触摸屏技术以及3）构建和测试集成的生物传感器和触摸屏 可以从患者样品中检测SARS-COV-2的阵列。这些目标成功完成 将为SARS-COV-2提供一个新型的自动传感平台。这项技术可以改变 个人设备触摸屏以及医院，机场，机场的多用户触摸屏设备 图书馆，餐馆，用于早期发现，从次生暴露中遏制传输率。 重要的是，开发的技术可以适用于其他电子传感平台， 并容易用于未来的病原体检测。