Synthetic hemostats for trauma treatment

描述：创伤性伤害，如道路交通事故或故意暴力行为，每年夺去约500万人的生命。出血占创伤相关死亡的30-40%，是受伤后最初6小时内死亡的主要原因。本项目的总体目标是开发一种基于生物聚合物的治疗方法，用于创伤医学，以加强血凝块并阻止内伤出血。这一点很重要，因为无法控制的出血是创伤受害者死亡的主要原因。我们的方法基于这样的知识，即血凝块内纤维蛋白纤维网络的结构是血凝块强度的重要组成部分，并且增强纤维蛋白凝块结构是减少血凝块分解和出血的一种经过验证的方法。我们将通过提供一种可溶的、与血液相容的聚合物来改变血块结构，这种聚合物可以识别并强化血块部位。这种新型聚合物与多种纤维蛋白特异性结合肽（FBP）功能化，使其能够与

DESCRIPTION: Traumatic injuries, such as from road traffic accidents or intentional acts of violence, claim approximately 5 million lives annually. Hemorrhage is responsible for 30-40% of trauma- associated deaths and is the leading cause of the death in the initial 6 hours after injury The overall goal of this project is to develop a biopolymer-based treatment for use in trauma medicine to strengthen blood clots and stop bleeding from internal wounds. This is important because uncontrollable bleeding is a major cause of death for trauma victims. We base our approach on the knowledge that the structure of the fibrin fiber network within a blood clot is an essential component of clot strength and that enhancing fibrin clot structure is a proven method for reducing clot breakdown and bleeding. We will alter clot structure by delivering a soluble and blood-compatible polymer that recognizes and reinforces clot sites. This novel polymer is functionalized with multiple fibrin-specific binding peptides (FBP's) allowing it to interface with fibrin during polymerization to alter clot structure, thus reinforcing the clot and making it more resistant to breakdown. We hypothesize that FBP-decorated biopolymers can enhance fibrin clot strength and reduce enzymatic clot breakdown (fibrinolysis). In this work, we will optimize structures of potential polymeric hemostats and evaluate these materials both in vitro and in vivo for their ability to stabilize clots and stop bleeding without associated toxicity and cardiopulmonary complications.