Touchscreen-compatible, real-time electrochemical sensing of SARS-CoV-2

项目摘要 引起COVID-19全球大流行的SARS-CoV-2冠状病毒正在有效传播 截至2020年9月8日，确诊病例已超过2700万例。因此， 迫切需要新的技术，可以提供早期检测病毒，减少 传播和感染率。该提案的目标是开发一种集成的生物传感器- 触摸屏灵敏度报告表面接触SARS-CoV-2。在我们的初步工作中， 我们已经鉴定了几种特异性结合锚定的三聚体刺突的适体 (S)SARS-CoV-2蛋白，但不是SARS-CoV或MERS蛋白。与抗体相比， 适体是合成分子，其更热稳定且成本更低，同时提供 靶结合的相似特异性和亲和力。在本申请中，我们建议将 基于适体的SARS-CoV-2的生物传感到触摸屏设备中。我们的主要目标是 1)用于电化学传感SARS-CoV-2的工程构象转换适体 结合，2）开发纳米间隙电容传感器作为一种独特的补充方法， 电容式触摸屏技术和3）构建和测试集成的生物传感器和触摸屏 可以从患者样本中检测SARS-CoV-2的阵列。圆满完成这些目标 将为SARS-CoV-2提供一个新的自动传感平台。这项技术可以改变 个人设备触摸屏以及医院，机场， 图书馆、餐馆，以便及早发现，遏制二次接触的传播率。 重要的是，开发的技术可以适用于其他电子传感平台， 并且易于应用于未来的病原体检测。

PROJECT SUMMARY The SARS-CoV-2 coronavirus, the cause of the COVID-19 global pandemic, is efficiently spread and has reached over 27 million confirmed cases as of September 8, 2020. There is therefore an urgent need for new technologies that can provide early detection of virus, reducing the transmission and infection rate. The goal of this proposal is to develop an integrated biosensor- touchscreen that sensitivity reports surface contact with SARS-CoV-2. In our preliminary work, we have identified several aptamers that bind specifically to the envelope-anchored trimeric spike (S) protein of SARS-CoV-2, but not of SARS-CoV or MERS. In comparison to antibodies, aptamers are synthetic molecules that more thermally stable and lower cost while providing similar specificity and affinity of target binding. In this application, we propose to integrate aptamer-based biosensing of SARS-CoV-2 into a touchscreen device. Our main objectives are to 1) engineer conformation switching aptamers for electrochemical sensing of SARS-CoV-2 binding, 2) develop nanogap capacitive sensors as a uniquely complementary approach to capacitive touchscreen technology and 3) build and test an integrated biosensor and touchscreen array that can detect SARS-CoV-2 from patient samples. Successful completion of these aims will result in a novel automatic sensing platform for SARS-CoV-2. This technology could transform personal device touchscreens as well as to multi-user touchscreen devices in hospitals, airports, libraries, restaurants, for early detection, curbing transmission rates from secondary exposure. Importantly, the developed technology could be adapted for other electronic sensing platforms, and easily applied for future pathogen detection.