Touchscreen-compatible, real-time electrochemical sensing of SARS-CoV-2
触摸屏兼容的 SARS-CoV-2 实时电化学传感
基本信息
- 批准号:10320986
- 负责人:
- 金额:$ 45.59万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2020
- 资助国家:美国
- 起止时间:2020-12-21 至 2023-11-30
- 项目状态:已结题
- 来源:
- 关键词:2019-nCoVAffinityAirAntibodiesBindingBinding ProteinsBiosensing TechniquesBiosensorCOVID-19COVID-19 detectionCOVID-19 pandemicCOVID-19 patientCar PhoneCellular PhoneCessation of lifeChemistryClinicalContact TracingCoronavirusDetectionDevelopmentDevicesDisease OutbreaksEarly DiagnosisElectric CapacitanceElectrodesElectronicsEngineeringEvaluationExhibitsFaceFrequenciesFutureGlassGoalsHospitalsImmobilizationIndividualInfectionLabelLibrariesMeasuresMiddle East Respiratory SyndromeMolecular ConformationNatural regenerationOxidation-ReductionPathogen detectionPatternPerformancePreparationProcessReportingRestaurantsSARS coronavirusSARS-CoV-2 spike proteinSARS-CoV-2 transmissionSamplingSensitivity and SpecificitySignal TransductionSpecificitySurfaceSystemTechnologyTestingTimeTouch sensationVaccinesValidationVirionWorkaptamerbasecostdensitydesigninfection rateinstrumentationnanofabricationnew technologynovelpreventsecondary infectionsensortouchscreentransmission processviral detectionviral transmission
项目摘要
PROJECT SUMMARY
The SARS-CoV-2 coronavirus, the cause of the COVID-19 global pandemic, is efficiently spread
and has reached over 27 million confirmed cases as of September 8, 2020. There is therefore an
urgent need for new technologies that can provide early detection of virus, reducing the
transmission and infection rate. The goal of this proposal is to develop an integrated biosensor-
touchscreen that sensitivity reports surface contact with SARS-CoV-2. In our preliminary work,
we have identified several aptamers that bind specifically to the envelope-anchored trimeric spike
(S) protein of SARS-CoV-2, but not of SARS-CoV or MERS. In comparison to antibodies,
aptamers are synthetic molecules that more thermally stable and lower cost while providing
similar specificity and affinity of target binding. In this application, we propose to integrate
aptamer-based biosensing of SARS-CoV-2 into a touchscreen device. Our main objectives are to
1) engineer conformation switching aptamers for electrochemical sensing of SARS-CoV-2
binding, 2) develop nanogap capacitive sensors as a uniquely complementary approach to
capacitive touchscreen technology and 3) build and test an integrated biosensor and touchscreen
array that can detect SARS-CoV-2 from patient samples. Successful completion of these aims
will result in a novel automatic sensing platform for SARS-CoV-2. This technology could transform
personal device touchscreens as well as to multi-user touchscreen devices in hospitals, airports,
libraries, restaurants, for early detection, curbing transmission rates from secondary exposure.
Importantly, the developed technology could be adapted for other electronic sensing platforms,
and easily applied for future pathogen detection.
项目摘要
引起COVID-19全球大流行的SARS-CoV-2冠状病毒正在有效传播
截至2020年9月8日,确诊病例已超过2700万例。因此,
迫切需要新的技术,可以提供早期检测病毒,减少
传播和感染率。该提案的目标是开发一种集成的生物传感器-
触摸屏灵敏度报告表面接触SARS-CoV-2。在我们的初步工作中,
我们已经鉴定了几种特异性结合锚定的三聚体刺突的适体
(S)SARS-CoV-2蛋白,但不是SARS-CoV或MERS蛋白。与抗体相比,
适体是合成分子,其更热稳定且成本更低,同时提供
靶结合的相似特异性和亲和力。在本申请中,我们建议将
基于适体的SARS-CoV-2的生物传感到触摸屏设备中。我们的主要目标是
1)用于电化学传感SARS-CoV-2的工程构象转换适体
结合,2)开发纳米间隙电容传感器作为一种独特的补充方法,
电容式触摸屏技术和3)构建和测试集成的生物传感器和触摸屏
可以从患者样本中检测SARS-CoV-2的阵列。成功完成这些目标
将为SARS-CoV-2提供一个新的自动传感平台。这项技术可以改变
个人设备触摸屏以及医院,机场,
图书馆、餐馆,以便及早发现,遏制二次接触的传播率。
重要的是,开发的技术可以适用于其他电子传感平台,
并且易于应用于未来的病原体检测。
项目成果
期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Aptamers 101: aptamer discovery and in vitro applications in biosensors and separations.
适体101:适体发现以及生物传感器和分离中的体外应用。
- DOI:10.1039/d3sc00439b
- 发表时间:2023-05-17
- 期刊:
- 影响因子:8.4
- 作者:
- 通讯作者:
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Suzie H. Pun其他文献
Enhanced angiogenesis mediated by vascular endothelial growth factor plasmid-loaded thermoresponsive amphiphilic hydrogel in rat myocardial infarction model
- DOI:
10.1016/j.carrev.2009.04.054 - 发表时间:
2009-07-01 - 期刊:
- 影响因子:
- 作者:
Jin Sook Kwon;In Kyu Park;Jung Jun Min;Myung Ho Jeong;Won Jong Kim;Seongbong Jo;Suzie H. Pun;Jeong Gwan Cho;Jong Chun Park;Jung Chaee Kang;Youngkeun Ahn - 通讯作者:
Youngkeun Ahn
172. Spatially-Controlled Delivery of Cyclodextrin-Based Polyplexes from Solid Surfaces
- DOI:
10.1016/j.ymthe.2006.08.196 - 发表时间:
2006-01-01 - 期刊:
- 影响因子:
- 作者:
In-Kyu Park;Horst A. von Recum;Shaoyi Jiang;Suzie H. Pun - 通讯作者:
Suzie H. Pun
394. Neuron-Specific, In Vivo Gene Delivery Mediated by Non-Viral Vectors
- DOI:
10.1016/j.ymthe.2006.08.456 - 发表时间:
2006-01-01 - 期刊:
- 影响因子:
- 作者:
In-Kyu Park;Jurate Lasiene;Jennifer M. Knox;Shinn-Huey Chou;Philip J. Horner;Suzie H. Pun - 通讯作者:
Suzie H. Pun
Suzie H. Pun的其他文献
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{{ truncateString('Suzie H. Pun', 18)}}的其他基金
Traceless, aptamer-based cell isolation for cell therapy applications
用于细胞治疗应用的无痕、基于适体的细胞分离
- 批准号:
10633756 - 财政年份:2023
- 资助金额:
$ 45.59万 - 项目类别:
An integrated polymeric carrier for subunit cancer vaccines
用于亚单位癌症疫苗的集成聚合物载体
- 批准号:
10366085 - 财政年份:2021
- 资助金额:
$ 45.59万 - 项目类别:
2022 Drug Carriers in Medicine and Biology GRC/GRS
2022年医学和生物学药物载体GRC/GRS
- 批准号:
10378904 - 财政年份:2021
- 资助金额:
$ 45.59万 - 项目类别:
An integrated polymeric carrier for subunit cancer vaccines
用于亚单位癌症疫苗的集成聚合物载体
- 批准号:
10576958 - 财政年份:2021
- 资助金额:
$ 45.59万 - 项目类别:
An integrated polymeric carrier for subunit cancer vaccines
用于亚单位癌症疫苗的集成聚合物载体
- 批准号:
10229292 - 财政年份:2021
- 资助金额:
$ 45.59万 - 项目类别:
Touchscreen-compatible, real-time electrochemical sensing of SARS-CoV-2
触摸屏兼容的 SARS-CoV-2 实时电化学传感
- 批准号:
10260946 - 财政年份:2020
- 资助金额:
$ 45.59万 - 项目类别:
POTENTIATING BREAST CANCER THERAPIES BY TARGETING TUMOR-ASSOCIATED MACROPHAGE
通过靶向肿瘤相关巨噬细胞来增强乳腺癌治疗
- 批准号:
8788393 - 财政年份:2014
- 资助金额:
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