Touchscreen-compatible, real-time electrochemical sensing of SARS-CoV-2

项目总结 导致新冠肺炎全球大流行的SARS-CoV-2冠状病毒正在有效传播 截至2020年9月8日，确诊病例已超过2700万例。因此，有一种 迫切需要能够提供早期病毒检测的新技术，以减少 传播率和感染率。这项提议的目标是开发一种集成的生物传感器- 触摸屏，灵敏度报告与SARS-CoV-2的表面接触。在我们的前期工作中， 我们已经确定了几个能与包膜锚定的三聚体刺突特异结合的适配子 (S)SARS-CoV-2的蛋白，而不是SARS-CoV或MERS的蛋白。与抗体相比， 适配子是一种合成分子，它具有更高的热稳定性和更低的成本，同时提供 与靶结合具有相似的特异性和亲和力。在本应用程序中，我们建议集成 基于适体的SARS-CoV-2生物传感到触摸屏设备。我们的主要目标是 1)用于SARS-CoV-2电化学传感的工程构象转换适配子 绑定，2)开发纳米间隙电容式传感器，作为一种独特的补充方法 电容式触摸屏技术以及3)构建和测试集成生物传感器和触摸屏 可以从患者样本中检测SARS-CoV-2的阵列。圆满完成这些目标 将产生一种针对SARS-CoV-2的新型自动传感平台。这项技术可能会改变 个人设备触摸屏以及医院、机场、 图书馆、餐馆，以便及早发现，遏制二次接触的传播率。 重要的是，开发的技术可以适用于其他电子传感平台， 并可方便地应用于未来的病原体检测。

PROJECT SUMMARY The SARS-CoV-2 coronavirus, the cause of the COVID-19 global pandemic, is efficiently spread and has reached over 27 million confirmed cases as of September 8, 2020. There is therefore an urgent need for new technologies that can provide early detection of virus, reducing the transmission and infection rate. The goal of this proposal is to develop an integrated biosensor- touchscreen that sensitivity reports surface contact with SARS-CoV-2. In our preliminary work, we have identified several aptamers that bind specifically to the envelope-anchored trimeric spike (S) protein of SARS-CoV-2, but not of SARS-CoV or MERS. In comparison to antibodies, aptamers are synthetic molecules that more thermally stable and lower cost while providing similar specificity and affinity of target binding. In this application, we propose to integrate aptamer-based biosensing of SARS-CoV-2 into a touchscreen device. Our main objectives are to 1) engineer conformation switching aptamers for electrochemical sensing of SARS-CoV-2 binding, 2) develop nanogap capacitive sensors as a uniquely complementary approach to capacitive touchscreen technology and 3) build and test an integrated biosensor and touchscreen array that can detect SARS-CoV-2 from patient samples. Successful completion of these aims will result in a novel automatic sensing platform for SARS-CoV-2. This technology could transform personal device touchscreens as well as to multi-user touchscreen devices in hospitals, airports, libraries, restaurants, for early detection, curbing transmission rates from secondary exposure. Importantly, the developed technology could be adapted for other electronic sensing platforms, and easily applied for future pathogen detection.