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Functional Genomics and Bioinformatics Core

功能基因组学和生物信息学核心

基本信息

批准号：
10259821
负责人：
Haotian Zhao
金额：
$ 40.66万
依托单位：
SANFORD RESEARCH/USD
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-09-01 至 2023-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10259821
关键词：
Address Applications Grants Binding Bioinformatics Biological Biological Assay Cells Centers of Research Excellence Childhood Chromatin Clustered Regularly Interspaced Short Palindromic Repeats Complex Core Facility Custom DNA DNA Sequencing Facility DNA-Protein Interaction Data Analyses Development Disease Environment Epigenetic Process Equipment Eukaryotic Cell Experimental Designs Fee-for-Service Plans Funding Gene Expression Gene Expression Profiling Genes Genetic Genetic Variation Genome Genome engineering Genomic Segment Genomics Goals Gold Guide RNA High-Throughput Nucleotide Sequencing Human Resources In Situ Hybridization Individual Infrastructure Institutes Investigation Investigator-Initiated Research Laboratories Manuscripts Mediating Medical Methods Molecular Molecular Biology Nucleic Acids Pattern Pediatric Research Performance Phase Physicians Preparation Principal Investigator Protocols documentation Publications RNA Research Research Personnel Research Support Resources Sampling Scientist Services Signal Transduction South Dakota Structure System Techniques Technology Time Tissues Training Transcript United States National Institutes of Health Work cell type chromatin immunoprecipitation data management design epigenetic variation epigenome experimental study flexibility functional genomics genetic manipulation genome editing high throughput screening histone modification large datasets microbial novel strategies nuclease operation preclinical study programs transcription factor transcriptome transcriptome sequencing

项目摘要

PROJECT SUMMARY The Functional Genomics and Bioinformatics Core facility in the Center for Pediatric Research offers efficient, high quality and flexible services in molecular and genomics assays for COBRE Project Leads and other researchers in Sanford Research and regional institutes. The establishment and operation of the Core provides a centralized resource for infrastructure, expertise, equipment and technical capabilities to perform molecular and functional genomics studies proposed by COBRE Project Leads. The Functional Genomics and Bioinformatics Core facility provides personalized services for investigators from experimental design to assay execution and data analysis. The state-of-the-art, high-throughput equipment in the laboratory enables a wide range of molecular and genomics assays, including nucleic acid isolation and quality analysis and manipulation, quantitative real-time PCR analysis, RNA in situ hybridization, chromatin immunoprecipitation studies, bioinformatics services, and to facilitate CRISPR/Cas9 mediated genome editing and. In addition, the Core facilitates high-throughput sequencing and bioinformatics studies. These services provided by the Core have contributed extensively to publications, grant applications, and preclinical studies by Project Leads. A plan is developed for a sustainable Core facility beyond COBRE funding period to meet the research demands of our scientists. By providing the support, resources and expert Core services, the Functional Genomics and Bioinformatics Core will enhance the overall institutional research capacity and promote the advancement of this core group of scientists and physicians to build a stronger pediatric research program, and ultimately increase the number of NIH-funded biomedical researchers in South Dakota.

项目摘要儿科研究中心的功能基因组学和生物信息学核心设施提供高效，为COBRE项目负责人和其他人提供分子和基因组学分析方面的高质量和灵活的服务桑福德研究所和地区研究所的研究人员。核心的建立和运作提供了基础设施、专业知识、设备和技术能力的集中资源，以及COBRE项目负责人提出的功能基因组学研究。功能基因组学和生物信息学核心设施为研究人员提供从实验设计到分析的个性化服务执行和数据分析。实验室中最先进的高通量设备可以实现广泛的一系列分子和基因组学检测，包括核酸分离和质量分析，实时荧光定量PCR分析，RNA原位杂交，染色质免疫沉淀研究，生物信息学服务，并促进CRISPR/Cas9介导的基因组编辑和。此外该 Core有助于高通量测序和生物信息学研究。核心小组提供的这些服务为项目负责人的出版物、资助申请和临床前研究做出了广泛贡献。一为满足研究需求，在COBRE资助期之后，制定了一个可持续的核心设施计划我们的科学家。通过提供支持，资源和专家核心服务，功能基因组学和生物信息学核心将提高整体机构的研究能力，并促进这个由科学家和医生组成的核心小组将建立一个更强大的儿科研究项目，在南达科他州增加NIH资助的生物医学研究人员的数量。