Molecular Genetics

项目总结(见说明)： Cobre申请的主要目标是促进儿科研究中心Cobre研究人员的发展，并帮助他们在研究计划中取得成功。这项研究将由桑福德研究/美国大学的一个多学科研究小组进行，他们致力于基础和转化性生物医学研究。为了促进南达科他州唯一的儿科基础和转化性研究多学科培训计划的发展，桑福德研究/美国联合大学将建立基础设施，为Cobre项目负责人提供必要的集中化实验室服务。分子遗传学核心设施是儿童健康研究中心的一个实验室，为桑福德研究中心的Cobre项目负责人和其他研究人员提供高效、高质量和负担得起的分子遗传学服务。实验室中最先进的高通量设备将使广泛的分子遗传学分析成为可能，包括从组织和细胞中提取核酸、核酸样本的定量和定性分析、基因表达分析和培训。分子遗传学核心设施将为Cobre项目负责人提供从实验设计到分析执行和数据分析的个性化服务。为满足Cobre项目负责人和桑福德研究/美国大学的其他研究人员的需求，制定了一项可持续的分子遗传学核心设施的计划。通过提供资金、资源和核心实验室服务，Sanford Research/U.S.将促进这一核心科学家群体的进步，以建立更强大的儿科研究计划，并最终增加NLH资助的南达科他州生物医学研究人员的数量。

PROJECT SUMMARY (See instructions): The major goal of this COBRE application is to foster the development of COBRE investigators in the Center of Pediatric Research and help them to succeed in their research programs. This will be carried out by a multidisciplinary group of researchers at Sanford Research/USD who are dedicated to basic and translational biomedical research. To promote the growth of the only multidisciplinary training program for pediatric basic and translational studies in South Dakota, the Sanford Research/USD will establish infrastructures to provide essential and centralized laboratory service to COBRE project leaders. The Molecular Genetics Core facility is a laboratory in the Children's Health Research Center offering efficient, high quality and affordable molecular genetics services for COBRE project leaders and other researchers in Sanford Research/USD. The state-of-the-art, high-throughput equipment in the laboratory will enable a wide range of molecular genetics assays, including extraction of nucleic acids from tissues and cells, quantitative and qualitative analysis of nucleic acid samples, gene expression analysis and training. The Molecular Genetics Core facility will provide personalized services for COBRE project leaders from experimental design to assay execution and data analysis. A plan is developed for a sustainable Molecular Genetics Core facility to serve the needs of COBRE project leaders and other researchers in Sanford Research/USD. By providing the funding, resources and core lab services, Sanford Research/USD will promote the advancement of this core group of scientists to build a stronger pediatric research program, and ultimately increase the number of NlH-funded biomedical researchers in South Dakota.