Structure and Function of Serum Amyloid A in Health and Disease

授予文摘

Awarded Abstract This research is focused on normal and pathologic actions of serum amyloid A (SAA), an enigmatic biomarker of inflammation and a protein precursor of AA amyloidosis, a life-threatening complication of chronic inflammation. SAA is a small soluble intrinsically disordered protein that increases rapidly and dramatically up to 1,000-fold in plasma following inflammation, infection or injury. The advantage for survival of this steep but transient increase is unclear, and the beneficial role of SAA in immune response is obscure. However, evolutionarily conserved aspects of the SAA structure, its lipophilic character, and the rapid and major commitment of liver and local tissues to SAA biosynthesis strongly support the importance of SAA-lipid interactions. Our new structural model suggests how SAA binds lipids and cell receptors, thereby rerouting lipid metabolism. Pilot biochemical studies compel us to propose a vital new role for SAA in clearing cellular membrane debris from the injured sites. Our powerful biophysical and biochemical approach will determine the mechanism via which SAA sequesters phospholipids and other lipids from cell membranes to facilitate their breakdown and safe removal from the injured sites in a process that is prerequisite for tissue healing. We will also determine how various lipids and other factors modulate amyloid formation by SAA, and thereby help decelerate or block this pathogenic process. Three complementary specific aims develop new concepts fundamental to SAA action in immune response and amyloid formation. Aim 1 will use an array of biophysical, structural and cell-based methods to establish molecular underpinnings for SAA interactions with its functional ligands, lipids and CD36 cell receptor. We will test our new structural model suggesting that SAA binds lipids via a unique apolar face whose shape defines protein's preference for binding to highly curved lipoproteins or forming them de novo to sequester lipids. We will determine the SAA conformation on the lipid and quantify its binding to CD36. Aim 2 will probe a novel synergy between SAA and secretory phospholipase A2 (sPLA2), an acute-phase plasma protein upregulated simultaneously with SAA in inflammation. Pilot studies compel us to propose a novel beneficial function of SAA in solubilizing phospholipids and their hydrolytic products to generate substrates for sPLA2 and to remove its products. The results will establish the hitherto unknown vital primordial role of SAA. Aim 3 will determine effects of various lipids, their degradation products, pH and heparin sulfate on SAA amyloid formation. We will also test our new idea that plasma lipids such as triacylglycerol modulate the SAA release from its major plasma carrier, high-density lipoprotein, and ultimately form amyloid. The results will help determine whether lipid-lowering therapies hold promise for treating AA amyloidosis. Completion of this research will establish raison d'etre for this enigmatic protein, provide a molecular basis for its action in immune response and lipid homeostasis, and help find much-needed new therapies or repurpose existing ones to treat a life-threatening human disease.