Metabolic Phenotyping Core

俄克拉荷马州细胞代谢研究中心(CMRO)第一阶段Cobre提案的目标是 培育以新陈代谢为重点的多学科创新研究。作为这个总体目标的一部分，我们创建了 代谢表型核心促进我们的Cobre研究人员向独立的R01基金过渡 并在俄克拉荷马州开展代谢研究。科布雷调查人员提出的项目寻求 了解细胞代谢过程驱动或导致心血管疾病的机制， 自身免疫性疾病和癌症。因为细胞新陈代谢是复杂的，并构成了许多生物和 技术挑战，核心将提供一支由训练有素的董事和博士级别的科学人员组成的团队， 到最先进的仪器，经过精心维护，并接受实验设计方面的指导培训， 新陈代谢数据的实施和解释。核心解决三个关键需求，以支持我们的 Cobre研究人员：1)评估细胞呼吸和线粒体功能，2)同位素代谢 示踪剂分析，以及3)基于代谢物的代谢图谱和生物信息学分析。为了满足这些需求， 我们的具体目标是：1)提供研究设计、分析优化、数据分析和 解释结果，以及评估和开发体内代谢表型的新方法， 和2)向Cobre调查人员提供中央设备和适当的科学和技术 培训以促进细胞代谢评估方面的新专门知识的发展。为了支持科布雷 研究人员表示，核心将提供细胞呼吸和线粒体功能的仪器(如： 海马、Oroboros和EPR)、代谢物示踪分析(LC-MS/MS)和代谢谱(GC-MS和 GC-MS/MS)。《核心》将由蒂莫西·格里芬博士执导，本杰明·米勒博士联合执导。两者都有 董事有超过20年的新陈代谢研究经验和先前的领导经验。 各就各位。核心有一个拟议的结构，以促进明确定义的支持人员的角色以及 透明的决策过程。通过这一结构，核心将促进指导和协作 文化，促进分享想法和方法的环境，并有助于建立一个可持续的 科布雷研究人员以及俄克拉荷马州其他研究人员的代谢研究资源。

The goal of the Center for Cellular Metabolism Research in Oklahoma (CMRO) Phase I COBRE proposal is to cultivate novel multidisciplinary research focusing on metabolism. As part of this overall goal, we created the Metabolic Phenotyping Core to facilitate the transition of our COBRE investigators to independent R01 funding and to develop metabolic research in Oklahoma. The projects proposed by the COBRE investigators seek to understand the mechanisms by which cellular metabolic processes drive or result from cardiovascular disease, autoimmune disease, and cancer. Because cellular metabolism is complex and poses many biological and technical challenges, the Core will provide a team of highly-trained directors and PhD-level scientific staff, access to state-of-the-art instruments that are carefully maintained, and mentored training in experimental design, implementation, and interpretation of metabolic data. The Core addresses three critical needs to support our COBRE investigators: 1) assessment of cellular respiration and mitochondrial function, 2) isotopic metabolic tracer analyses, and 3) metabolite-based metabolic profiling and bioinformatic analyses. To meet these needs, our specific aims are to: 1) provide expertise with study design, assay optimization, data analysis, and interpretation of results, as well as evaluate and develop new methodologies for in vivo metabolic phenotyping, and 2) provide COBRE investigators access to centralized equipment and appropriate scientific and technical training to facilitate the development of new expertise in cellular metabolic assessment. To support the COBRE Investigators, the Core will provide instrumentation for cellular respiration and mitochondrial function (e.g. Seahorse, Oroboros, and EPR), metabolite tracer analysis (LC-MS/MS), and metabolic profiling (GC-MS and GC-MS/MS). The Core will be directed by Dr. Timothy Griffin and Co-directed by Dr. Benjamin Miller. Both directors have over 20 years of experience in studies of metabolism and previous experience in leadership positions. The Core has a proposed structure that facilitates clearly-defined roles of support staff as well as transparent decision-making processes. Through this structure, the Core will foster a mentoring and collaborative culture, promote an idea- and method-sharing environment, and contribute to the establishment of a sustainable resource for metabolic research for COBRE Investigators as well as other researchers in Oklahoma.