Metabolic Phenotyping Core

代谢表型核心

• 批准号: 10571892
• 负责人: TIMOTHY M GRIFFIN
• 金额: $ 30.06万
• 依托单位: OKLAHOMA MEDICAL RESEARCH FOUNDATION
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-02-05 至 2026-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10571892
• 关键词: Address; Advisory Committees; Aging; Area; Autoimmune Diseases; Bioinformatics; Biological; Biological Assay; Cardiovascular Diseases; Cell Respiration; Cells; Cellular Metabolic Process; Centers of Research Excellence; Clinical Trials; Complex; Consultations; Data; Data Analyses; Decision Making; Degenerative polyarthritis; Development; Diet; Disease; Doctor of Philosophy; Ensure; Environment; Equipment; Evaluation; Experimental Designs; Fostering; Functional disorder; Funding; Future; Genus Hippocampus; Goals; Growth; Interdisciplinary Study; Isotopes; Leadership; Malignant Neoplasms; Mass Fragmentography; Measurement; Mediating; Mentors; Mentorship; Metabolic; Metabolism; Metformin; Methodology; Methods; Mitochondria; Modeling; Mus; Obese Mice; Oklahoma; Pathology; Phase; Physiology; Positioning Attribute; Procedures; Process; Regulation; Research; Research Design; Research Personnel; Research Support; Resources; Role; Services; Standardization; Structure; Technical Expertise; Techniques; Tissues; Tracer; Training; analytical method; comparative; design; exercise intervention; experience; follow-up; in vivo; instrument; instrumentation; interest; meetings; member; metabolic abnormality assessment; metabolic phenotype; mouse development; multidisciplinary; new technology; novel; operation; programs; protein degradation; skills; stable isotope; sustainable resource

The goal of the Center for Cellular Metabolism Research in Oklahoma (CMRO) Phase I COBRE proposal is to cultivate novel multidisciplinary research focusing on metabolism. As part of this overall goal, we created the Metabolic Phenotyping Core to facilitate the transition of our COBRE investigators to independent R01 funding and to develop metabolic research in Oklahoma. The projects proposed by the COBRE investigators seek to understand the mechanisms by which cellular metabolic processes drive or result from cardiovascular disease, autoimmune disease, and cancer. Because cellular metabolism is complex and poses many biological and technical challenges, the Core will provide a team of highly-trained directors and PhD-level scientific staff, access to state-of-the-art instruments that are carefully maintained, and mentored training in experimental design, implementation, and interpretation of metabolic data. The Core addresses three critical needs to support our COBRE investigators: 1) assessment of cellular respiration and mitochondrial function, 2) isotopic metabolic tracer analyses, and 3) metabolite-based metabolic profiling and bioinformatic analyses. To meet these needs, our specific aims are to: 1) provide expertise with study design, assay optimization, data analysis, and interpretation of results, as well as evaluate and develop new methodologies for in vivo metabolic phenotyping, and 2) provide COBRE investigators access to centralized equipment and appropriate scientific and technical training to facilitate the development of new expertise in cellular metabolic assessment. To support the COBRE Investigators, the Core will provide instrumentation for cellular respiration and mitochondrial function (e.g. Seahorse, Oroboros, and EPR), metabolite tracer analysis (LC-MS/MS), and metabolic profiling (GC-MS and GC-MS/MS). The Core will be directed by Dr. Timothy Griffin and Co-directed by Dr. Benjamin Miller. Both directors have over 20 years of experience in studies of metabolism and previous experience in leadership positions. The Core has a proposed structure that facilitates clearly-defined roles of support staff as well as transparent decision-making processes. Through this structure, the Core will foster a mentoring and collaborative culture, promote an idea- and method-sharing environment, and contribute to the establishment of a sustainable resource for metabolic research for COBRE Investigators as well as other researchers in Oklahoma.

俄克拉荷马州细胞代谢研究中心（CMRO）第一阶段COBRE提案的目标是 培养新的多学科研究，重点是新陈代谢。作为这一总体目标的一部分，我们创建了 代谢表型分析核心，以促进我们的COBRE研究人员过渡到独立的R 01资金 并在俄克拉荷马州开展代谢研究。COBRE调查人员提出的项目旨在 了解细胞代谢过程驱动或导致心血管疾病的机制， 自身免疫性疾病和癌症由于细胞代谢是复杂的，并造成许多生物和 技术挑战，核心将提供一支训练有素的董事和博士级科学工作人员，访问 到精心维护的最先进的仪器，以及实验设计的指导培训， 代谢数据的实现和解释。核心解决了三个关键需求，以支持我们的 COBRE研究人员：1）评估细胞呼吸和线粒体功能，2）同位素代谢 示踪剂分析，和3）基于代谢物的代谢谱分析和生物信息学分析。为了满足这些需求， 我们的具体目标是：1）提供研究设计，分析优化，数据分析的专业知识， 结果解释，以及评估和开发体内代谢表型分析的新方法， 2）为COBRE调查人员提供集中设备和适当的科学技术 培训，以促进细胞代谢评估的新专业知识的发展。支援眼镜蛇 研究人员，核心将提供细胞呼吸和线粒体功能的仪器（例如， 海马、Oroboros和EPR）、代谢物示踪分析（LC-MS/MS）和代谢谱分析（GC-MS和 GC-MS/MS）。核心将由蒂莫西格里芬博士和本杰明米勒博士共同指导。两 董事们在新陈代谢研究方面有20多年的经验， 岗位核心小组有一个拟议的结构，便于明确界定支助人员的作用， 透明的决策过程。通过这一结构，核心将促进指导和协作， 文化，促进思想和方法共享的环境，并有助于建立一个可持续的 COBRE调查员以及俄克拉荷马州的其他研究人员的代谢研究资源。