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Loss of progenitor function accelerates lung aging

祖细胞功能丧失加速肺部衰老

基本信息

批准号：
10579157
负责人：
SUSAN M MAJKA
金额：
$ 69.86万
依托单位：
NATIONAL JEWISH HEALTH
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-06-15 至 2028-02-29
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10579157
关键词：
5 year old Acceleration Address Adult Age Aging Biological Assay Biology Blood Vessels Cardiovascular system Cell Communication Cell Count Cell physiology Cells Cellular Structures Characteristics Chronic Chronic Obstructive Pulmonary Disease Chronic lung disease Coculture Techniques Coupled Data Development Disease Distal Endothelium Female Functional disorder Goals Histologic Homeostasis Human Immune Impairment In Vitro Inflammation Inflammatory Inflammatory Infiltrate Interleukin-13 Knowledge Link Lung Lung Lavage Fluid Lung diseases Lymphoid Lymphoid Cell Maintenance Medial Mesenchymal Modeling Mus Oxidation-Reduction Patients Phenotype Physiological Physiology Plasma Play Population Predisposition Process Prognosis Proteins Pulmonary Emphysema Sampling Senility Smoker Smoking Stromal Cells Structure Structure of parenchyma of lung Subgroup Testing Tissues Vascular Diseases Vascular remodeling Work aged aging population angiogenesis cytokine epithelial stem cell exhaustion exposure to cigarette smoke human model immune cell infiltrate in vivo in vivo evaluation knock-down male mouse model novel premature progenitor pulmonary function stem cell function stem cells transcriptomics

项目摘要

Aging is associated with loss of lung structure and declining function. Emphysematous loss of tissue structure is exacerbated by vasculopathy, which increases susceptibility to lung disease, and limits survival. We have previously demonstrated that the structure and function of the lung microvasculature is regulated by a specialized mesenchymal vascular progenitor cell (MVPC). We have also defined Dickkopf-related protein 1 (DKK1) as a regulator of this niche in murine and human model. Our preliminary data demonstrate that MVPC numbers decline with age in WT mice by 1 year, and that, when MVPC are depleted in young mice, lung aging is accelerated, resulting in severe emphysema at 1.5 years of age. While MVPC are key modulators of the pulmonary microvasculature in the distal lung, adventitial stem cells (ASC) influence large blood vessel homeostasis in the proximal lung. Our preliminary data identifies key similarities between MVPCs and ASCs suggesting that decline in ASC numbers and function may also result in accelerated lung aging. Given ASC function is tightly controlled by lung-resident type-2 innate lymphoid cells (ILC2) and pulmonary ILC2 are known to decrease with age, loss of ASC-ILC2 crosstalk is likely a contributor to emphysema. Similar to aging, vascular remodeling is driven by chronic activation of type 2 (Th2) inflammation. Our preliminary data support increased Th2 cytokine profiles in plasma and BAL of COPD patients. Human and murine scRNAseq data define ILC2 and MVPC populations as well as immune cell infiltrate and cytokine expression characteristic of Th2 inflammation in COPD and a smoking model. The goal of this proposal is to define the mechanisms that result in loss of adult MVPC and ILC2 progenitor function contributing to accelerated lung aging, by altering vascular structure and function, and increased susceptibility to emphysema in the aged population. We hypothesize that loss of progenitor function accelerates lung aging by altering cell interactions within vascular niches, promoting vascular remodeling and increasing susceptibility to emphysema in the aging population. We will test that decline in MVPC and ILC2 numbers and/or function in the aging lung accelerates aging via impairment of vascular homeostasis due to disruption of cell – cell interactions in their respective perivascular niches, promoting vascular remodeling and loss of tissue structure using novel conditional murine models to knock down progenitors in aged mice or young mice. We will assess the requirement of MVPC, MVPC derived DKK1, ILC2 cells, and ILC2 derived IL13 in the maintenance of vascular niche homeostasis and susceptibility to emphysema. We will use conditional models to manipulate DKK1 or IL13 expression in mice allowed to age in the presence or absence of cigarette smoke exposure both in vivo and in vitro. Lastly, we will test that loss of ILC2 and MVPC progenitors promotes lung aging and is associated with COPD and a type-2 immune endotype. This work will provide an understanding of progenitor aging, mechanisms by which loss of MVPC and ILC2 function drives vascular remodeling with a Th2 endotype contributing to lung aging and emphysema.

衰老与肺结构丧失和功能下降有关。肺气肿性组织结构丧失