Core B: Proteomics and Metabolomics

核心B总结 蛋白质组学和代谢组学核心（核心B）提供了最先进的仪器，方法， 在分析蛋白质组学和代谢组学的专业知识，在这个PPG的研究人员。的独特特征 蛋白质组学和代谢组学核心是提供尖端的定量蛋白质组学分析 这些技术允许在胃癌模型中高灵敏度地测量蛋白质组变化。新 这项更新申请的服务包括发现和靶向代谢组学以及组织成像 质谱法（IMS）。该核心提供1）复杂样品中蛋白质的鉴定和定量， 2)蛋白质修饰分析，包括磷酸化，3）代谢物定量分析 丰度，和4）使用IMS的胃组织的分子成像。 核心工作人员提供实验设计方面的咨询，以及样品制备、质量控制、 光谱分析和原始数据分析。多台高性能质谱仪用于LC- MS/MS实验可用于拟定分析。此外，先进的方法是可用的 包括：多维LC-MS/MS，用于复杂样品的蛋白质鉴定，TagRecon数据库 搜索分析，以从MS/MS数据中鉴定非预期修饰或序列变体，以及 用于组织分析的高空间和质量分辨率成像方法。核心还提供相对 使用稳定同位素差异标记策略iTRAQ和SILAC对来自 动物模型或细胞培养物。蛋白质表达或代谢产物丰度的验证 使用靶向多反应监测LC-MS/MS方法完成变化。 项目1（Peek）将利用代谢组学方法测量H.分离的幽门螺杆菌菌株 从感染的铁耗尽或充满沙鼠作为时间的函数。项目2（Wilson）将利用磷-SILAC 测量磷酸化蛋白质组学和代谢组学的变化， 巨噬细胞对H. pylori感染和EGFR或DHPS抑制。项目3（封面）将采用 代谢组学方法来检查血浆和胃组织中的代谢物变化。此外，该项目 将利用IMS方法在空间上测量胃组织中代谢物、蛋白质和脂质的变化， 解决的方式作为饮食盐，感染和炎症的功能。 !

Core B Summary The Proteomics and Metabolomics Core (Core B) provides state-of-the-art instrumentation, methodology, and expertise in analytical proteomics and metabolomics to investigators in this PPG. A unique feature of the Proteomics and Metabolomics Core is the availability of cutting-edge quantitative proteomics analysis technologies that allows high sensitivity measurements of proteome changes in models of gastric cancer. New services in this renewal application include discovery and targeted metabolomics as well as tissue imaging mass spectrometry (IMS). The Core provides 1) identification and quantitation of proteins in complex samples, 2) analyses of protein modifications, including phosphorylation, 3) quantitative analyses of metabolite abundances, and 4) molecular imaging of gastric tissue using IMS. Core staff provide consultation on experimental design as well as hands on sample preparation, mass spectrometry analysis and primary data analysis. Multiple high performance mass spectrometers for LC- MS/MS experiments are available for proposed analyses. In addition, advanced methods are available including: multidimensional LC-MS/MS for protein identification from complex samples, TagRecon database searching analysis for identification of unanticipated modifications or sequence variants from MS/MS data, and high spatial and mass resolution imaging methods for tissue analysis. The Core also provides relative quantitation using stable isotope differential labeling strategies, iTRAQ and SILAC, for samples derived from animal models or from cell culture, respectively. Validation of protein expression or metabolite abundance changes is accomplished using targeted multiple reaction monitoring LC-MS/MS methods. Project 1 (Peek) will utilize metabolomics methods to measure metabolite changes in H. pylori strains isolated from infected iron deplete or replete gerbils as a function of time. Project 2 (Wilson) will utilize phospho-SILAC to measure changes in the phosphoproteome and metabolomics to measure metabolite changes in macrophages upon H. pylori infection and with EGFR or DHPS inhibition. Project 3 (Cover) will employ metabolomics methods to examine metabolite changes in plasma and gastric tissue. In addition, this project will utilize IMS methods to measure changes in metabolites, proteins and lipids in gastric tissue in a spatially- resolved manner as a function of dietary salt, infection and inflammation. !