Eye Organ Specific Project
眼器官特定项目
基本信息
- 批准号:10704490
- 负责人:
- 金额:$ 82.26万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2020
- 资助国家:美国
- 起止时间:2020-09-30 至 2024-08-31
- 项目状态:已结题
- 来源:
- 关键词:3-DimensionalAddressAffectAfrican American populationAgeAge FactorsAge related macular degenerationAmericanAnalytical ChemistryAnteriorAntibodiesAqueous HumorArchitectureAtlasesBiological AssayBrainCataractCell CountCell SeparationCellsCessation of lifeCiliary BodyClinicalCollectionCorneaCytometryDataData AnalysesData ScienceDefectDepositionDetectionDevelopmentDiagnostic ImagingDimensionsDiseaseDissociationEmerging TechnologiesEpidemiologyExtracellular MatrixEyeEye diseasesFluorescenceFoundationsGene Expression ProfilingGeneticGlaucomaGleanGoalsHeterogeneityHistocytochemistryHistologicHourHumanHuman BioMolecular Atlas ProgramHuman ResourcesImageImmunofluorescence ImmunologicInvestigationIonsKidneyKnowledgeLifeLightLinkLipidsLiquid substanceLocationMapsMass Spectrum AnalysisMetabolicMethodsMicroscopyMolecularMolecular AnalysisMolecular ProfilingMorphologyMultimodal ImagingNormalcyOphthalmologyOptical Coherence TomographyOpticsOrganPancreasPatientsPhotonsPreparationPropertyProteinsQuality ControlQuality of lifeRaceRecording of previous eventsResearchResearch PersonnelResolutionRetinaRetinal PhotoreceptorsSamplingSensorySignal TransductionSpecificitySpecimenSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationSpeedStainsStandardizationStructureStructure of retinal pigment epitheliumSunlightSurfaceSystemTechnologyTimeTissue BanksTissue atlasTissuesTrabecular meshwork structureVisionVisualVisual impairmentVitreous humorWorkabsorptionbiobankcell typeclinical diagnosticsdata qualitydemographicsdesignexperimental studyfortificationhigh intraocular pressurehigh riskhuman imagingimaging facilitiesimaging modalityindexinginsightinstrumentationlensmaculamass spectrometric imagingmolecular imagingmultimodalitynovel therapeuticsprofessorprogramsreconstructionsexspatial relationshipthree dimensional structuretissue mappingtransmission process
项目摘要
PROJECT SUMMARY – Eye Organ Specific Project. Comprehensive 3-dimensional biomolecular analysis
within tissues requires the coordination of data from many analytical technologies. We will develop a multimodal
strategy to obtain spatially specific ‘omics’ information directly from human whole eye tissue. This work will
leverage the unique instrumentation capabilities and expertise of the Mass Spectrometry Research Center
(MSRC) in which Professors Spraggins, Caprioli, Schey, Gutierrez and Van de Plas are key personnel. Building
from previous work developing atlases of human kidney tissue, we will integrate ultra-high speed, high spatial
resolution (<10μm) MALDI timsTOF IMS with high mass resolution (>40,000 Resolving Power, <2 ppm mass
accuracy) for molecular imaging of human eyes with an unprecedented combination of spatial fidelity and
molecular specificity. The high-throughput capabilities of MALDI timsTOF IMS will be critical for generating high-
spatial resolution 3-D molecular images from statistically relevant numbers of normal pancreas samples. MALDI
IMS will provide the specificity and mass accuracy necessary to link ion images to orthogonal LC-based
fragmentation experiments for molecular identification. Fragmentation will be performed using spatially specific
surface sampling approaches to produce LC-MS/MS data from tissues in a manner that is compatible with IMS
and capable of being integrated into HuBMAP tissue atlases. To maximize information gleaned from imaging
experiments and lay the groundwork for data-driven image fusion and 3-D reconstruction outlined in the Data
Analysis Core Research Strategy, we will also develop methods for collecting various microscopy-based image
modalities (e.g. autofluorescence and stained microscopy) and pancreas specific, highly multiplexed
immunofluorescence using CODEX. Whole eye atlases will be constructed by combining our multimodal
IMS/Microscopy pipelines with ex vivo 3-D optical coherence tomography (OCT) imaging.
Through the use of our multimodal pipeline, we propose to map spatial relationships of eye cell types
from all regions of the eye within healthy tissue across a multitude of demographics. Aim 1: To create an eye-
specific biospecimen collection and management plan. Aim 2: To establish a pre-analytical pipeline for
standardizing sample preparation, determining tissue normalcy, and providing quality control metrics. Aim 3:
To develop a multimodal characterization pipeline for eye-specific 3-D molecular imaging. Aim 4: To scale and
standardize eye-specific 3-D molecular imaging. To accomplish these aims, we have assembled a highly
interactive and established team of investigators consisting of complementary expertise in ophthalmology,
analytical chemistry, and data science.
项目摘要-眼器官专项项目。全面的三维生物分子分析
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Kevin L Schey其他文献
Kevin L Schey的其他文献
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{{ truncateString('Kevin L Schey', 18)}}的其他基金
High resolution Thermo Scientific Q-Exactive Orbitrap mass spectrometer for metabolomics
用于代谢组学的高分辨率 Thermo Scientific Q-Exactive Orbitrap 质谱仪
- 批准号:
9274461 - 财政年份:2017
- 资助金额:
$ 82.26万 - 项目类别:
Mechanisms of Protein Aging in Normal and Cataractous Lenses
正常和白内障晶状体蛋白质老化的机制
- 批准号:
8669561 - 财政年份:2014
- 资助金额:
$ 82.26万 - 项目类别:
Mechanisms of Protein Aging in Normal and Cataractous Lenses
正常和白内障晶状体蛋白质老化的机制
- 批准号:
9313263 - 财政年份:2014
- 资助金额:
$ 82.26万 - 项目类别:
Mechanisms of Protein Aging in Normal and Cataractous Lenses
正常和白内障晶状体蛋白质老化的机制
- 批准号:
10386818 - 财政年份:2014
- 资助金额:
$ 82.26万 - 项目类别:
Mechanisms of Protein Aging in Normal and Cataractous Lenses
正常和白内障晶状体蛋白质老化的机制
- 批准号:
9916774 - 财政年份:2014
- 资助金额:
$ 82.26万 - 项目类别:
Proteome and Transcriptome Markers of Hypertension in Urine and Plasma Exosomes
尿液和血浆外泌体中高血压的蛋白质组和转录组标志物
- 批准号:
7815243 - 财政年份:2009
- 资助金额:
$ 82.26万 - 项目类别:
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