High resolution Thermo Scientific Q-Exactive Orbitrap mass spectrometer for metabolomics

用于代谢组学的高分辨率 Thermo Scientific Q-Exactive Orbitrap 质谱仪

• 批准号: 9274461
• 负责人: Kevin L Schey
• 金额: $ 52.43万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-05-01 至 2018-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9274461
• 关键词: 10 year old; Cancer Center; Coupled; Diabetes Mellitus; Digestive System Disorders; Engineering; Eye diseases; Fee Schedules; Funding; Hybrids; Institution; Maintenance; Malignant Neoplasms; Mass Spectrum Analysis; Minor; Obesity; Performance; Research; Research Personnel; Resolution; System; Training; United States National Institutes of Health; Vision research; age related; instrument; mass spectrometer; member; metabolomics; programs; repaired; small molecule

Project Summary Proposed here is the purchase of a Thermo Scientific hybrid quadrupole-orbitrap Q Exactive Plus mass spectrometer coupled with a Thermo Scientific Vanquish UHPLC system for discovery and targeted metabolomics analyses. The requested instrument will support a large number of NIH funded investigators including six major users and six minor users with ongoing metabolomics projects. These projects vary in focus from cancer, diabetes, obesity, to age- related eye diseases. Furthermore, investigators from the Diabetes Training and Research Center, the Digestive Disease Center, the Vanderbilt Ingram Cancer Center, and the Vanderbilt Vision Research Center will benefit from the new capabilities. The Q Exactive Plus system will be placed in the small molecule Mass Spectrometry Core of the Vanderbilt Mass Spectrometry Research Center. The instrument will be managed by a team of four professionals in the Core including the Associate Director responsible for training users, a technician responsible for instrument QA/QC and maintenance, an engineer responsible for instrument repair, and the PI responsible for instrument oversight. Operational funds will be generated from the Core's standard fee schedule as established by the Vanderbilt Office of Research and as approved by the Core's oversight committee. In addition, an instrument advisory panels will be established and consist of major users, a member of the institution's Core administration, and a metabolomics expert. The primary purpose of the instrument will be to replace 8-10 year old instruments and to provide state-of-the-art sensitivity and overall performance to enhance the research programs of twelve investigators and three major centers.

项目摘要 这里提出的是购买热科学混合四极轨道阱Q Exactive Plus质谱仪与Thermo Scientific Vanquish UHPLC系统耦合，用于 发现和靶向代谢组学分析。所要求的仪器将支持大型 NIH资助的研究人员人数，包括6名主要用户和6名次要用户， 代谢组学项目。这些项目的重点各不相同，从癌症，糖尿病，肥胖，到年龄- 相关眼病。此外，糖尿病培训和研究中心的研究人员 中心、消化疾病中心、范德比尔特英格拉姆癌症中心和范德比尔特 视觉研究中心将受益于新功能。Q Exactive Plus系统将 放置在范德比尔特质谱仪的小分子质谱仪核心中 研究中心该工具将由核心小组的四名专业人员管理。 包括负责培训用户的副总监、负责 仪器QA/QC和维护，负责仪器维修的工程师和PI 负责仪器监督。业务资金将来自核心方案的 由范德比尔特研究办公室制定并经 核心的监督委员会此外，还将设立一个文书咨询小组， 由主要用户、机构核心管理层成员和 代谢组学专家该仪器的主要目的将是取代8-10岁 仪器，并提供最先进的灵敏度和整体性能，以提高 12名研究人员和三个主要中心的研究计划。