A MALDI INSTRUMENT FOR TISSUE IMAGING & PROTEIN PROFILING: CARDIOVASCULAR

这个子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是用于拨款的，而不一定是用于调查人员的机构。描述(申请人提供)：MALDI组织成像和MALDI组织图谱代表了强大的新方法，其中空间分辨蛋白质图谱可以直接从组织样本中实现。在成像模式下，这些轮廓可以被组装成一个蛋白质特定的组织图像，相当于免疫组织化学实验，但被多路复用1000倍。直接从组织中以高空间分辨率绘制蛋白质图谱的能力有可能识别疾病的生物标志物，并识别以下特定机制：1)疾病，2)组织分化，以及3)药物反应和毒性。本申请描述了对购买最先进的MALDI组织成像仪器的请求，该仪器用于心血管、癌症和眼科重要样本的蛋白质图谱和组织成像。具体地说，布鲁克Ultraflex II，MALDI串联式飞行时间仪器被要求允许目前可能的最高速度图像采集。此外，还需要用于成像和侧写采集和分析的支持软件。这项强大的技术预计将对南卡罗来纳医科大学的主要研究实力领域产生重大影响，如本提案中所述的NIH支持的12个研究项目。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): MALDI tissue imaging and MALDI tissue profiling represent powerful new methods where spatially resolved protein profiling can be achieved directly from tissue samples. In imaging mode the profiles can be assembled into a protein specific tissue image equivalent to immunohistochemical experiments but multiplexed 1,000 fold. The ability to map protein profiles with high spatial resolution directly from tissues has the potential to identify biomarkers of disease, and to identify specific mechanisms of: 1) disease, 2) tissue differentiation, as well as 3) drug response and toxicity. This application describes the request for acquisition of a state-of-the-art MALDI tissue imaging instrument for protein profiling and tissue imaging of samples of cardiovascular, cancer and ophthalmologic importance. Specifically, the Bruker Ultraflex II, MALDI tandem time-of-flight instrument is requested to allow the highest speed image acquisition currently possible. In addition, supported software for both imaging and profiling acquisition and analysis are requested. This powerful technology is expected to have significant impact on areas of major research strength at the Medical University of South Carolina as represented by the 12 NIH supported research projects described in this proposal.