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Genome Editing

基因组编辑

基本信息

批准号：
10580681
负责人：
Derek B. Sant'Angelo
金额：
$ 12.43万
依托单位：
RUTGERS BIOMEDICAL AND HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-03-01 至 2024-02-29
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10580681
关键词：
Animal Model Bacterial Artificial Chromosomes Breeding CRISPR/Cas technology Cancer Center Support Grant Cancer Institute of New Jersey Cancer Model Cancer Patient Cell Line Cell division Cell model Cells Clone Cells Clustered Regularly Interspaced Short Palindromic Repeats Complex Consultations Cryopreservation Custom DNA DNA Sequence Development Disease Electroporation Ensure Equilibrium Equipment Generations Genes Genetically Engineered Mouse Health Heritability Housing Human Human Cell Line Individual Institution Laboratories Leadership Malignant Neoplasms Mediating Methodology Methods Microinjections Modeling Molecular Biology Mouse Cell Line Mouse Strains Mus Mutant Strains Mice Mutation Phenotype Play Preparation Procedures Process Reagent Research Research Personnel Research Project Grants Resource Sharing Resources Role Services Speed Technical Degree Technical Expertise Technology Training and Education Transgenic Mice Validation base editing cancer initiation design embryo cryopreservation embryonic stem cell experience genome editing germ free condition human cancer mouse model in vivo innovation member mouse genome mouse model novel diagnostics novel strategies novel therapeutics nuclease precise genome editing preservation skills sperm cryopreservation tool tumor tumor progression tumorigenesis zygote

项目摘要

! GENOME EDITING SHARED RESOURCE PROJECT SUMMARY/ABSTRACT Mouse models of human cancer are instrumental in understanding tumorigenesis. Advances continue to be made that allow better understanding of the mechanisms of tumor development, which leads toward the identification of new diagnostics and therapies. The Genome Editing shared resource at Rutgers Cancer Institute of New Jersey (CINJ) was established in 2016 as a consortium managed shared resource whose purpose is to provide a centralized laboratory for the generation and preservation of mouse research models and creation of custom genome edited cell lines. The services offered by the Genome Editing shared resource ensure that investigators across the consortium are equipped to make scientific breakthroughs and speed research into mechanisms of cancer initiation, progression and treatment using genetically engineered mouse models (GEMMs) and genetically modified human and mouse cell lines. The considerable experience and technical expertise of the managing and scientific directors are also a valuable resource made available to investigators. Moreover, Genome Editing offers cryopreservation of research mouse models to safeguard against devastating loss of investigators’ valuable GEMM lines. Almost all of the procedures conducted by the shared resource require a high degree of technical expertise and equipment not readily available to individual laboratories. In addition to its service functions, the Genome Editing shared resource plays an important intellectual role in the development and pursuit of mouse-oriented research projects and the application of genome editing throughout the CINJ consortium. The shared resource also engages in the development of new approaches and methodologies in genome editing to better serve its users.

! 基因组编辑共享资源项目总结/摘要人类癌症的小鼠模型有助于理解肿瘤发生。进步继续这使得人们能够更好地了解肿瘤发展的机制，这将导致确定新的诊断和治疗方法。基因组编辑在罗格斯癌症共享资源新泽西研究所（CINJ）成立于2016年，是一个管理共享资源的财团，目的是为小鼠研究模型的生成和保存提供一个集中的实验室和创建定制基因组编辑的细胞系。基因组编辑共享资源提供的服务确保整个联盟的研究人员有能力取得科学突破，加快对癌症发生机制的研究，使用基因工程小鼠模型（GEMM）和基因修饰的人和小鼠细胞系。管理人员的丰富经验和技术专长，科学主任也是研究人员可以利用的宝贵资源。此外，基因组编辑提供研究小鼠模型的冷冻保存，以防止研究人员的毁灭性损失。宝贵的GEMM线路。几乎所有由共享资源执行的程序都需要高度的个别实验室不容易获得的技术专门知识和设备。除了其服务功能外，基因组编辑共享资源在以下方面发挥着重要的智力作用：发展和追求以小鼠为导向的研究项目和基因组编辑的应用在CINJ联盟中。共享资源也参与新方法的开发以及基因组编辑的方法，以更好地为用户服务。