Metabolic Physiology and Energy Balance Core

代谢生理学和能量平衡核心

• 批准号: 10586204
• 负责人: BARBARA B. KAHN
• 金额: $ 18.35万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-05-15 至 2028-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10586204
• 关键词: Adipocytes; Adipose tissue; Affect; Area; Biological; Body Composition; Body Temperature; Body Weight decreased; Body measure procedure; Bone Density; Boston; Carbon Dioxide; Cell model; Cells; Communities; Continuous Glucose Monitor; Diabetes Mellitus; Dose; Dual-Energy X-Ray Absorptiometry; Eating; Endocrinology; Ensure; Equipment; Fatty Acids; Feedback; Genes; Genetic Vectors; Genomics; Glucose; Goals; Grant; Homeostasis; In Vitro; Indirect Calorimetry; Insulin; Insulin Resistance; Knock-in; Lipids; Lipolysis; Measurement; Measures; Metabolic; Metabolism; Mission; Modeling; Mus; Obesity; Oxygen Consumption; Pathway interactions; Phenotype; Physical activity; Physiology; Production; Proteomics; Quality Control; Reproducibility; Research; Research Design; Research Personnel; Resolution; Rodent Model; Role; Services; Surveys; Techniques; Technology; Training and Education; Triglycerides; United States National Institutes of Health; Viral Vector; Weight Gain; awake; blood glucose regulation; design; energy balance; experience; experimental study; gene product; glucose metabolism; glucose transport; glucose uptake; in vivo; insulin sensitivity; knockout gene; lipid metabolism; member; metabolic rate; metabolomics; mouse model; overexpression; respiratory; response; tool

METABOLIC PHYSIOLOGY AND ENERGY BALANCE CORE Project Summary/Abstract The main function of the Metabolic Physiology and Energy Balance Core is to perform experimental services to investigate alterations of glucose homeostasis, insulin sensitivity, lipid metabolism and body composition in rodent models of obesity and insulin resistance. Increasingly, the biologic roles of gene products and pathways emerging from genomic, proteomic, metabolomic and other discovery approaches are being queried by generating overexpression, gene knockout or knock-in of altered genes in rodent models. Thus, there is a growing need for characterization of new mouse models created by genetic or viral vector technologies. The Metabolic Physiology and Energy Balance Core is designed to meet the needs for characterization of rodent models that have alterations in glucose homeostasis, insulin action, and/or body composition and adiposity. The specific objectives of the Core are to carry out and interpret experiments for Core users aimed at investigating glucose homeostasis and energy balance. In vivo studies in awake mice include continuous glucose monitoring, measuring metabolic rates and energy balance by indirect calorimetry and body composition by EchoMRI and DEXA. Studies in primary adipocytes or adipose explants include glucose transport, metabolism, and lipolysis with assessment of insulin sensitivity. Services also include the differentiation of stromovascular cells into adipocytes and assessment of glucose uptake and insulin sensitivity. There is high demand for these services and we anticipate high utilization. The Core is actively utilized by BADERC members and other investigators in the Boston area. It enables investigators from other fields to carry out studies in the area of diabetes and metabolism that may not otherwise be feasible. Since the inception of the Core, we have received an increasing number of requests for services from investigators new to the field of metabolism, diabetes and obesity. The Core provides critical support for NIH grants for both new and established investigators. In many cases, the Core provides the tools that enable experts in other fields to apply their sophisticated techniques to metabolism research.

代谢生理学和能量平衡核心 项目摘要/摘要 新陈代谢生理和能量平衡核心的主要功能是执行实验服务 探讨啮齿类动物糖稳态、胰岛素敏感性、脂代谢和体成分的变化 肥胖和胰岛素抵抗的模型。 基因组、蛋白质组、代谢组学产生的基因产物和途径的生物学作用日益突出 而其他的发现方法正在通过产生过度表达、基因敲除或敲入 改变了啮齿动物模型中的基因。因此，越来越需要对所创建的新的鼠标模型进行表征 通过基因或病毒载体技术。新陈代谢生理和能量平衡核心旨在满足 需要对葡萄糖稳态、胰岛素作用和/或身体发生变化的啮齿动物模型进行表征 成分和肥胖。 核心的具体目标是为核心用户开展和解释旨在调查的实验 葡萄糖平衡和能量平衡。清醒小鼠的活体研究包括连续血糖监测， 用间接量热法测量代谢率和能量平衡，用EchoMRI和DEXA测量身体成分。 在原代脂肪细胞或脂肪外植体中的研究包括葡萄糖的运输、代谢和脂肪分解。 胰岛素敏感性评估。服务还包括将基质血管细胞分化为脂肪细胞。 并评估葡萄糖摄取和胰岛素敏感性。对这些服务的需求很高，我们预计 高利用率。 波士顿地区的BADERC成员和其他调查人员积极利用该核心。它实现了 来自其他领域的研究人员在糖尿病和新陈代谢领域进行研究，否则可能不会 可行。自核心项目成立以来，我们收到了越来越多的服务请求 新陈代谢、糖尿病和肥胖症领域的研究人员。核心为美国国立卫生研究院的拨款提供关键支持 无论是新手还是老牌调查员。在许多情况下，核心提供的工具使其他领域的专家能够 将他们的尖端技术应用于新陈代谢研究。