Metabolic Physiology and Energy Balance Core

代谢生理学与能量平衡核心 项目总结/摘要 代谢生理学和能量平衡核心的主要功能是执行实验服务 研究啮齿类动物血糖稳态、胰岛素敏感性、脂代谢和体成分的变化 肥胖和胰岛素抵抗的模型。 越来越多的基因产物和途径的生物学作用出现在基因组，蛋白质组，代谢组， 和其他发现方法正在通过产生过表达，基因敲除或敲入来质疑。 在啮齿动物模型中改变基因。因此，越来越需要表征所创建的新小鼠模型 通过基因或病毒载体技术。代谢生理学和能量平衡核心旨在满足 需要表征具有葡萄糖稳态、胰岛素作用和/或身体改变的啮齿动物模型 组成和肥胖。 核心的具体目标是为核心用户进行和解释旨在调查的实验， 葡萄糖稳态和能量平衡。在清醒小鼠中的体内研究包括连续葡萄糖监测， 通过间接量热法测量代谢率和能量平衡，通过EchoMRI和DEXA测量身体组成。 在原代脂肪细胞或脂肪外植体中的研究包括葡萄糖转运、代谢和脂肪分解， 评估胰岛素敏感性。服务还包括基质血管细胞分化为脂肪细胞 以及葡萄糖摄取和胰岛素敏感性的评估。对这些服务的需求很大，我们预计 利用率高。 BADERC成员和波士顿地区的其他调查人员积极利用该核心。它使 研究人员从其他领域进行研究，在该地区的糖尿病和代谢，否则可能不会 可行自核心小组成立以来，我们收到越来越多来自 新的代谢，糖尿病和肥胖症领域的研究人员。核心为NIH赠款提供关键支持 对于新的和现有的调查人员。在许多情况下，核心提供的工具，使专家在其他 将他们的尖端技术应用于新陈代谢研究。

METABOLIC PHYSIOLOGY AND ENERGY BALANCE CORE Project Summary/Abstract The main function of the Metabolic Physiology and Energy Balance Core is to perform experimental services to investigate alterations of glucose homeostasis, insulin sensitivity, lipid metabolism and body composition in rodent models of obesity and insulin resistance. Increasingly, the biologic roles of gene products and pathways emerging from genomic, proteomic, metabolomic and other discovery approaches are being queried by generating overexpression, gene knockout or knock-in of altered genes in rodent models. Thus, there is a growing need for characterization of new mouse models created by genetic or viral vector technologies. The Metabolic Physiology and Energy Balance Core is designed to meet the needs for characterization of rodent models that have alterations in glucose homeostasis, insulin action, and/or body composition and adiposity. The specific objectives of the Core are to carry out and interpret experiments for Core users aimed at investigating glucose homeostasis and energy balance. In vivo studies in awake mice include continuous glucose monitoring, measuring metabolic rates and energy balance by indirect calorimetry and body composition by EchoMRI and DEXA. Studies in primary adipocytes or adipose explants include glucose transport, metabolism, and lipolysis with assessment of insulin sensitivity. Services also include the differentiation of stromovascular cells into adipocytes and assessment of glucose uptake and insulin sensitivity. There is high demand for these services and we anticipate high utilization. The Core is actively utilized by BADERC members and other investigators in the Boston area. It enables investigators from other fields to carry out studies in the area of diabetes and metabolism that may not otherwise be feasible. Since the inception of the Core, we have received an increasing number of requests for services from investigators new to the field of metabolism, diabetes and obesity. The Core provides critical support for NIH grants for both new and established investigators. In many cases, the Core provides the tools that enable experts in other fields to apply their sophisticated techniques to metabolism research.