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Metabolic Physiology Core

代谢生理学核心

基本信息

批准号：
7925277
负责人：
BARBARA B. KAHN
金额：
$ 35.15万
依托单位：
MASSACHUSETTS GENERAL HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-05-01 至 2015-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7925277
关键词：
Address Adipocytes Adipose tissue Animal Model Animals Area Arts Biological Biological Assay Biopsy Body Composition Body Weight Bone Density Boston Cannulas Catheters Cell Nucleus Consultations Coulter counter Custom DEXA Data Development Diabetes Mellitus Dissection Doctor of Philosophy Eating Educational process of instructing Energy Metabolism Equipment Experimental Designs Fatty Acids Fatty acid glycerol esters Funding Genetically Engineered Mouse Glucose Glucose tolerance test Grant Homeostasis Hormones Human Hyperglycemia Hypoglycemia Hypothalamic structure In Vitro Individual Infusion procedures Injection of therapeutic agent Institutes Insulin Insulin Resistance International Intravenous Journals Knockout Mice Laboratories Lead Leptin Leptin resistance Letters Manuscripts Measurement Measures Metabolic Metabolism Methods Modeling Mus Neuropeptides Non-Insulin-Dependent Diabetes Mellitus Nonesterified Fatty Acids Obesity Operative Surgical Procedures Paper Peptides Peripheral Phosphorylation Physiology Postdoctoral Fellow Preparation Process Production Protocols documentation Publications Publishing Pyruvate Pyruvates Rattus Research Research Design Research Personnel Rodent Rodent Model Role STAT3 gene Sampling Services Signal Pathway Signal Transduction Skeletal Muscle Techniques Testing Thinness Time Tissue Transplantation Tissues Transgenic Mice Transgenic Organisms Triglycerides United States National Institutes of Health Variant Work Writing adenylate kinase blood glucose regulation design energy balance experience fatty acid metabolism gene discovery glucose production glucose transport glucose uptake implantation in vitro Assay in vivo insulin sensitivity insulin sensitivity/resistance insulin signaling insulin tolerance knockout gene lipid biosynthesis lipid metabolism meetings member metabolic abnormality assessment metabolomics mouse model overexpression research study response subcutaneous tool

项目摘要

The Animal Metabolic Physiology Core is designed to meet the needs for characterization of rodent models that have alterations in glucose homeostasis, insulin action, leptin action and/or body composition. Its main function is to teach and provide consultation on ex vivo and in vivo methods to investigate the mechanisms for metabolic alterations in genetically-manipulated mouse models and in dietary models of obesity/leanness or insulin resistance/sensitivity. The specific objectives of the Core are 1) to teach and 2) to provide consultation to investigators in designing, carrying out and interpreting in vitro and in vivo studies to investigate the action of insulin and hormones that regulate energy balance such as leptin; glucose homeostasis; lipid metabolism; and body composition; 3) The Core also performs a limited number of these studies on selected mouse or rat models created by or obtained by members of the Center. Services of the Core include ex vivo methods such as adipocyte isolation, and dissection and incubation of isolated skeletal muscles to study glucose transport, insulin signaling, leptin signaling, and glucose and fatty acid metabolism. In vivo methods assessing insulin action and glucose homeostasis include insulin tolerance test, glucose tolerance test, signaling assays in response to insulin injection or infusion, and measurement of glucose turnover, endogenous glucose production and glucose uptake by individual tissues during clamp studies. In vivo methods to investigate biological actions of orexigenic and anorexigenic hormones include measurement of food intake, energy expenditure and leptin signaling in peripheral tissues and hypothalamic nuclei. The Core also provides specialized equipment such as a DEXA scanner for non-invasive analysis of body composition and bone mineral density and a Coulter Counter for determining adipocyte number and size distribution. The Core Director, Barbara B. Kahn, MD, the Associate Core Director, Odile Peroni, PhD and the Research Associates who work with the Core have extensive experience with the metabolic and signaling assays offered by the Core. They are developing new assays to characterize additional aspects of integrated fuel metabolism and enerov homeostasis.

动物代谢生理学核心是为了满足在葡萄糖稳态、胰岛素作用、瘦素作用和/或身体组成方面发生变化的啮齿动物模型的特征描述的需要。它的主要功能是教授和提供关于体外和体内方法的咨询，以研究基因操纵的小鼠模型和肥胖/瘦或胰岛素抵抗/敏感的饮食模型中代谢变化的机制。该中心的具体目标是1)指导和2)向研究人员提供咨询，以设计、实施和解释体外和体内研究，以调查胰岛素和调节能量平衡的激素的作用，如瘦素、葡萄糖稳态、脂肪代谢和身体组成；3)中心还对中心成员创建或获得的选定小鼠或大鼠模型进行有限数量的此类研究。 Core的服务包括体外方法，如脂肪细胞分离，以及分离和孵化分离的骨骼肌，以研究葡萄糖运输、胰岛素信号、瘦素信号以及葡萄糖和脂肪酸代谢。体内评价胰岛素作用和葡萄糖稳态的方法包括胰岛素耐量试验、葡萄糖耐量试验、对胰岛素注射或输注反应的信号分析，以及在钳夹研究中测量葡萄糖周转、内源性葡萄糖产量和单个组织对葡萄糖的摄取。体内研究食欲素和厌食症激素的生物学作用的方法包括测量食物摄入量、能量消耗和周围组织和下丘脑的瘦素信号。原子核。Core还提供专门的设备，如用于非侵入性分析身体成分和骨密度的DEXA扫描仪，以及用于确定脂肪细胞数量和大小分布的库尔特计数器。Core董事Barbara B.Kahn医学博士、Core副董事、Odile Peroni博士和与Core一起工作的Research Associates在Core提供的代谢和信号分析方面拥有丰富的经验。他们正在开发新的分析方法，以表征综合燃料代谢和能量动态平衡的其他方面。