Cell Biology Core

细胞生物学核心

• 批准号: 10586202
• 负责人: Dennis Brown
• 金额: $ 13.02万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-05-15 至 2028-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10586202
• 关键词: Address; Antibodies; Area; Attention; Award; Biological; Blood Vessels; Boston; Cells; Cellular Immunology; Cellular Metabolic Process; Cellular biology; Communication; Communities; Competence; Confocal Microscopy; Core Facility; Data Reporting; Diabetes Mellitus; Electron Microscopy; Endocrinology; Endothelial Cells; Ensure; Environment; Equipment; Floor; Fluorescence Recovery After Photobleaching; Fluorescence Resonance Energy Transfer; Funding; Genetic; Goals; Grant; Human Resources; Image; Imaging Techniques; Immunofluorescence Immunologic; Incubated; Individual; Institution; Islet Cell; Journals; Laboratories; Lasers; Maintenance; Medicine; Membrane Biology; Membrane Proteins; Microdissection; Microscopy; Morphology; Nature; Procedures; Protocols documentation; Publications; Qualifying; Quality Control; Reporting; Reproducibility; Research; Research Personnel; Resources; Science; Signal Transduction; Structure; System; Targeted Research; Technical Expertise; Techniques; Temperature; Time; Training; United States National Institutes of Health; Work; base; cellular imaging; community center; data management; design; experience; high resolution imaging; imaging approach; instrumentation; programs; real-time images; recruit; stem cells; success; trafficking

PROJECT SUMMARY/ABSTRACT The Cell Biology/Morphology Core provides BADERC investigators with technical support, equipment, advice and expertise for cell biological, immunocytochemical and morphological studies related to cell/membrane biology and signal transduction; cellular immunology and islet cell biology; and vascular and endothelial cell biology. The Core is located within the MGH Program in Membrane Biology facility on the 8th floor of the Simches Research Center, which is adjacent to the MGH main campus. The Core Director, Dr. Brown, is an expert in the area of membrane biology, membrane proteins, and intracellular trafficking. He has a long and well-documented track record of operating Core facilities at MGH over the past 25 years, including this BADERC Core for over 20 years. The goal of this Core is to provide access to integrated cellular imaging solutions and related expertize for problems in Diabetes and other BADERC-targeted research areas that few or no isolated laboratories have the resources to develop individually. The Cell Biology Core is designed, therefore, to maximize the use and availability of specialized pieces of equipment, that are not only prohibitively expensive for many laboratories, but that also require expensive and regular maintenance, and appropriate technical expertise to use. The Core performs all aspects of microscopy from routine immunofluorescence imaging, confocal microscopy, and (immuno)electron microscopy. The Core is especially proud of its record in successfully attracting NIH Shared Instrumentation Grants (SIGs) and institutional support over the years. With support of Core users, we have obtained 6 SIGs in little more than 12 years, the most recent of which was a $450K award in 2016 for a new Zeiss LSM800 Airyscan confocal platform that offers high sensitivity/high resolution imaging and analysis capabilities to BADERC users. Our other major confocal platform, a Nikon A1R confocal system, offers real time imaging in a temperature controlled chamber, fluorescence resonance energy transfer (FRET) and fluorescence recovery after photobleaching (FRAP) and a SIG (for $300K) that was submitted in May 2021 to request major upgrades received an outstanding score of 20 and should be funded. We also offer laser cut microdissection and total internal reflection imaging. We also offer routine embedding and sectioning, and basic immunofluorescence incubation expertize. A major and important objective of the Core is to provide training of key personnel from participating laboratories, a strategy that has worked very successfully over the prior funding periods. Dr. Brown and his highly-qualified technical support staff have well-established and proven procedures for maximizing Core use in the context of varied projects and specific experimental requirements. We pay special attention to quality control procedures for image capture and analysis, and we have protocols for antibody verification to comply with current Rigor and Reproducibility standards. The bottom line is that this Core continues to work smoothly, as in the past. Its use has resulted in several hundred publications over that time that contain images taken by Core staff, or by trained users, including over 90 in the previous BADERC funding period. We expect this to continue and increase based on the usage proposed in this application.

项目摘要/摘要 细胞生物学/形态学核心为BADERC调查人员提供技术支持，设备，建议 和细胞生物学，免疫细胞化学和形态学研究的专业知识与细胞/膜有关 生物学和信号转导；细胞免疫学和胰岛细胞生物学；和血管和内皮细胞 生物学。核心位于Simches 8楼的膜生物学设施的MGH计划中 研究中心，毗邻MGH主校园。核心主任布朗博士是 膜生物学，膜蛋白和细胞内运输区域。他有一个漫长的记录 在过去25年中，MGH的操作核心设施的记录记录，其中包括20多个Baderc Core 年。该核心的目的是提供对集成的蜂窝成像解决方案的访问，并相关专业化 很少或没有孤立的实验室的糖尿病和其他针对BADERC的研究领域的问题 用于单独发展的资源。因此，设计了细胞生物学核心，以最大化使用和 可用性的专用设备，这些设备不仅对许多实验室而言非常昂贵，而且很昂贵 但这也需要昂贵且定期的维护以及适当的技术专业知识。核心 从常规免疫荧光成像，共聚焦显微镜和 （免疫）电子显微镜。核心为成功吸引NIH共享的记录特别自豪 多年来，仪器赠款（SIG）和机构支持。在核心用户的支持下，我们有 在12年中获得了6个Sigs Zeiss LSM800 Airyscan共聚焦平台，可提供高灵敏度/高分辨率成像和分析 Baderc用户的功能。我们的另一个主要共聚焦平台Nikon A1R共聚焦系统，可实时 温度控制室中的成像，荧光共振能量转移（FRET）和荧光 光漂白后的恢复（FRAP）和SIG（30万美元）于2021年5月提交，要求专业 升级获得了20分，应获得资金。我们还提供激光切割微分解 和总内反射成像。我们还提供常规的嵌入和切片以及基本 免疫荧光孵育专家。核心的主要和重要目标是提供培训 参与实验室的关键人员，该策略在先前的资金方面非常成功地发挥了作用 时期。布朗博士及其备受合格的技术支持人员拥有良好且可靠的程序 为了在各种项目和特定的实验要求的背景下最大化核心使用。我们支付特别的费用 注意图像捕获和分析的质量控制程序，我们有抗体方案 验证以符合当前严格和可重复性标准。最重要的是，此核心继续 像过去一样顺利工作。在那段时间内，它的使用导致了数百个出版物 由核心人员或受过训练的用户拍摄的图像，包括以前的BADERC资助期内90多个。我们 希望根据本应用程序中提出的用法继续增加并增加。