A Zeiss LSM800 confocal microscope with Airyscan

配备 Airyscan 的 Zeiss LSM800 共焦显微镜

• 批准号: 9075249
• 负责人: Dennis Brown
• 金额: $ 37.37万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-04-01 至 2017-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9075249
• 关键词: 14 year old; Cells; Communities; Confocal Microscopy; Core Facility; Ensure; Fluorescence Resonance Energy Transfer; Funding; General Hospitals; Health; Housing; Image; Imaging Techniques; Individual; Institution; Leadership; Life; Massachusetts; Medical Research; Membrane Biology; Microscope; Microscopy; Peer Review; Preparation; Publications; Research; Resolution; Services; Specimen; System; Techniques; Time; United States National Institutes of Health; base; detector; fluorescence imaging; imaging platform; improved; instrument; live cell imaging; member; microscopic imaging; multidisciplinary; programs; ranpirnase; repaired; research study

 DESCRIPTION (provided by applicant): We request funds to purchase a Zeiss LSM800 Confocal Microscope system with Airyscan to replace our 14 year old Zeiss/BioRad Radiance system that Zeiss can no longer guarantee to support or repair. The new instrument will be housed within the Program in Membrane Biology (PMB) Microscopy Core at the Massachusetts General Hospital (MGH) in the multidisciplinary Simches Research Center building. The mandate of this MGH approved Core facility is to provide access to a full range of techniques using microscopy and imaging to 2 NIH funded P30 Centers, Thematic Center members in the Simches building, and individual research groups within the MGH community and beyond. The PMB Core has developed considerable expertise in static and live cell imaging techniques that that been used by our extensive user base over the years. Our sophisticated imaging capabilities were enhanced 4 years ago by the incorporation of a Nikon A1R confocal platform to function alongside our older Zeiss Radiance, and together these instruments have been able to provide sufficient imaging bandwidth to serve our users. However, the Nikon is extensively used for longer term, live cell imaging, including FRET and FRAP experiments on live cells, while many of our users require instead consistent and timely access to a simpler confocal to perform more basic static imaging of fixed specimens. Unfortunately, we can no longer promise our many users that such access will continue after any given point in time, based on the fact that Zeiss can no longer guarantee to repair the Radiance. Therefore, the purpose of this application is: 1) To ensure that we can continue to provide routine confocal microscopy services to our extensive Core user base in the face of an old and potentially non repairable existing system; 2) To expand our Core capabilities to provide higher sensitivity and higher resolution (via the Airyscan detector) to our user base that will expand their imaging capabilities for exploring cellular details more precisely. The Airyscan detector provides a remarkable increase in resolution that is achieved easily and without the expense and specimen preparation issues that are associated with many other super resolution techniques. Since the PMB Core was founded in 1986, it has become a central part of the MGH/HMS medical research community. Over 37 NIH funded PIs and their numerous lab members within the HMS/MGH and in other institutions made significant user of the Core confocal in the past 12 months alone, and we have supported literally hundreds of peer review publications over the years. The Core has an ongoing quest to constantly improve and upgrade Core facilities in support of its user base. This has been achieved using funds from NIH SIG applications as well as considerable institutional support from the MGH leadership. Providing our Core users with continued access to an up to date fluorescence imaging platform is essential to maintaining the MGH community at the cutting edge of NIH funded, health related research.

 描述（由申请人提供）：我们请求资金购买带有 Airyscan 的 Zeiss LSM800 共焦显微镜系统，以替换我们已有 14 年历史的 Zeiss/BioRad Radiance 系统，而 Zeiss 无法再保证支持或维修该系统。 新仪器将安装在马萨诸塞州总医院 (MGH) 多学科 Simches 研究中心大楼的膜生物学 (PMB) 显微镜核心项目中。 这个 MGH 批准的核心设施的任务是为 2 个 NIH 资助的 P30 中心、Simches 大楼的主题中心成员以及 MGH 社区内外的各个研究小组提供使用显微镜和成像的全套技术。 PMB Core 在静态和活细胞成像技术方面积累了丰富的专业知识，多年来我们广泛的用户群一直在使用这些技术。 四年前，我们通过将 Nikon A1R 共焦平台与旧款 Zeiss Radiance 一起使用，增强了我们先进的成像能力，这些仪器共同能够提供足够的成像带宽来为我们的用户服务。 然而，尼康广泛用于长期活细胞成像，包括活细胞上的 FRET 和 FRAP 实验，而我们的许多用户需要一致且及时地使用更简单的共聚焦来对固定样本执行更基本的静态成像。 不幸的是，我们无法再向众多用户保证此类访问将在任何给定时间点后继续进行，因为蔡司无法再保证修复 Radiance。 因此，该应用程序的目的是： 1）确保我们在面对旧的且可能无法修复的现有系统时，能够继续为我们广泛的 Core 用户群提供常规共焦显微镜服务； 2) 扩展我们的核心功能，为我们的用户群提供更高的灵敏度和更高分辨率（通过 Airyscan 探测器），从而扩展他们的成像能力 更精确地探索细胞细节。 Airyscan 检测器显着提高了分辨率，并且可以轻松实现，并且没有与许多其他超分辨率技术相关的费用和样品制备问题。 自 1986 年 PMB Core 成立以来，它已成为 MGH/HMS 医学研究界的核心部分。 仅在过去 12 个月内，就有超过 37 名 NIH 资助的 PI 及其众多 HMS/MGH 和其他机构的实验室成员大量使用了 Core 共焦，多年来我们已经支持了数百份同行评审出版物。 Core 不断寻求不断改进和升级 Core 设施以支持其用户群。 这是通过 NIH SIG 申请的资金以及 MGH 领导层的大力机构支持来实现的。 为我们的核心用户提供持续访问最新荧光成像平台对于保持 MGH 社区处于 NIH 资助的健康相关研究的前沿至关重要。