A Zeiss LSM800 confocal microscope with Airyscan

配备 Airyscan 的 Zeiss LSM800 共焦显微镜

• 批准号: 9075249
• 负责人: Dennis Brown
• 金额: $ 37.37万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-04-01 至 2017-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9075249
• 关键词: 14 year old; Cells; Communities; Confocal Microscopy; Core Facility; Ensure; Fluorescence Resonance Energy Transfer; Funding; General Hospitals; Health; Housing; Image; Imaging Techniques; Individual; Institution; Leadership; Life; Massachusetts; Medical Research; Membrane Biology; Microscope; Microscopy; Peer Review; Preparation; Publications; Research; Resolution; Services; Specimen; System; Techniques; Time; United States National Institutes of Health; base; detector; fluorescence imaging; imaging platform; improved; instrument; live cell imaging; member; microscopic imaging; multidisciplinary; programs; ranpirnase; repaired; research study

 DESCRIPTION (provided by applicant): We request funds to purchase a Zeiss LSM800 Confocal Microscope system with Airyscan to replace our 14 year old Zeiss/BioRad Radiance system that Zeiss can no longer guarantee to support or repair. The new instrument will be housed within the Program in Membrane Biology (PMB) Microscopy Core at the Massachusetts General Hospital (MGH) in the multidisciplinary Simches Research Center building. The mandate of this MGH approved Core facility is to provide access to a full range of techniques using microscopy and imaging to 2 NIH funded P30 Centers, Thematic Center members in the Simches building, and individual research groups within the MGH community and beyond. The PMB Core has developed considerable expertise in static and live cell imaging techniques that that been used by our extensive user base over the years. Our sophisticated imaging capabilities were enhanced 4 years ago by the incorporation of a Nikon A1R confocal platform to function alongside our older Zeiss Radiance, and together these instruments have been able to provide sufficient imaging bandwidth to serve our users. However, the Nikon is extensively used for longer term, live cell imaging, including FRET and FRAP experiments on live cells, while many of our users require instead consistent and timely access to a simpler confocal to perform more basic static imaging of fixed specimens. Unfortunately, we can no longer promise our many users that such access will continue after any given point in time, based on the fact that Zeiss can no longer guarantee to repair the Radiance. Therefore, the purpose of this application is: 1) To ensure that we can continue to provide routine confocal microscopy services to our extensive Core user base in the face of an old and potentially non repairable existing system; 2) To expand our Core capabilities to provide higher sensitivity and higher resolution (via the Airyscan detector) to our user base that will expand their imaging capabilities for exploring cellular details more precisely. The Airyscan detector provides a remarkable increase in resolution that is achieved easily and without the expense and specimen preparation issues that are associated with many other super resolution techniques. Since the PMB Core was founded in 1986, it has become a central part of the MGH/HMS medical research community. Over 37 NIH funded PIs and their numerous lab members within the HMS/MGH and in other institutions made significant user of the Core confocal in the past 12 months alone, and we have supported literally hundreds of peer review publications over the years. The Core has an ongoing quest to constantly improve and upgrade Core facilities in support of its user base. This has been achieved using funds from NIH SIG applications as well as considerable institutional support from the MGH leadership. Providing our Core users with continued access to an up to date fluorescence imaging platform is essential to maintaining the MGH community at the cutting edge of NIH funded, health related research.

 描述(由申请人提供)：我们申请资金购买蔡司LSM800共焦显微镜系统和Airycan，以更换我们已有14年历史的蔡司/BioRad Radiance系统，蔡司不再能保证支持或维修该系统。新仪器将安装在马萨诸塞州综合医院(MGH)多学科西姆斯研究中心大楼的膜生物学(PMB)显微核心项目中。MGH批准的这一核心设施的任务是向NIH资助的两个P30中心、Simches大楼的主题中心成员以及MGH社区内外的各个研究小组提供使用显微镜和成像的全方位技术。PMB Core在静态和活体细胞成像技术方面发展了相当多的专业知识，这些技术多年来被我们广泛的用户基础所使用。4年前，尼康A1R共焦平台的加入增强了我们复杂的成像能力，使其能够与我们老式的蔡司Radiance一起工作，这些仪器一起能够提供足够的成像带宽来服务我们的用户。然而，尼康被广泛用于更长期的活细胞成像，包括活细胞上的FRET和FRAP实验，而我们的许多用户需要一致和及时地使用更简单的共焦来对固定样本执行更基本的静态成像。遗憾的是，基于蔡司不能再保证修复Radiance的事实，我们不能再向我们的许多用户承诺，在任何给定的时间点后，这种访问将继续进行。因此，此应用程序的目的是：1)确保面对老旧且可能无法修复的现有系统，我们能够继续为我们广泛的核心用户群提供常规的共焦显微镜服务；2)扩展我们的核心能力，为我们的用户群提供更高的灵敏度和更高的分辨率(通过AiryScan探测器)，从而扩展他们的成像能力 以便更精确地探索细胞细节。AiryScan探测器显著提高了分辨率，这一点很容易实现，而且不会产生与许多其他超分辨率技术相关的费用和样品制备问题。自1986年PMB Core成立以来，它已成为MGH/HMS医学研究社区的核心部分。仅在过去的12个月里，超过37个由NIH资助的PIs和他们在HMS/MGH和其他机构中的众多实验室成员使核心共同关注的重要用户，我们在过去的几年里支持了数百种同行评议出版物。核心不断改进和升级核心设施，以支持其用户基础。这是利用来自NIH SIG申请的资金以及MGH领导层的相当大的机构支持来实现的。为我们的核心用户提供持续访问最新的荧光成像平台，对于保持MGH社区在NIH资助的健康相关研究的前沿至关重要。