HD Upgrade to a Nikon A1R Confocal Imaging Platform

高清升级至尼康 A1R 共焦成像平台

基本信息

  • 批准号:
    10415591
  • 负责人:
  • 金额:
    $ 29.61万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2022
  • 资助国家:
    美国
  • 起止时间:
    2022-05-01 至 2023-04-30
  • 项目状态:
    已结题

项目摘要

Project Summary We request funds to support the upgrade of our existing Nikon A1R confocal microscope, which is now almost 10 years old and lacks many of the newer features that have been developed since its purchase in 2012. The upgraded instrument will be housed within the Program in Membrane Biology (PMB) Microscopy Core at the Massachusetts General Hospital (MGH) in the multidisciplinary Simches Research Center building. The mandate of this MGH-approved Core facility is to provide access to a full range of microscopy techniques to 2 NIH funded P30 Centers and individual research groups within the MGH community and beyond. The Core has considerable expertise in static and live cell imaging techniques used by our extensive user-base over the years. Our imaging capabilites were enhanced 4 years ago by the acquisition of a Zeiss LSM800 Airyscan confocal platform to function alongside the older Nikon A1R. Together these instruments have provided sufficient high level imaging bandwidth to serve our users. The Nikon A1R is extensively used for longer term, live cell imaging and has an inverted microscope and an environmental chamber to faciliate this need. The LSM800, associated with an upright microscope and no environmental chamber, provides consistent and timely access to a high resolution confocal that is mostly used to perform static imaging of fixed specimens. With the increase in demand for imaging of living cells expressing various types of fluorescent molecules and tagged proteins, and the increasing need to image these cells over time using 3D reconstructions, the purpose of this application is to provide our users with access to modern confocal microscopy options that are better adapted for specialized imaging purposes than the current older A1R system. In particular, the new high speed HD resonant scanning mode with associated AI image processing capabilities provides a remarkable increase in image size and resolution when compafed to the old resonant scanning system. Coupled with replacement of the old PMT detectors with new GaAsp detectors and the incorporation of stable solid state lasers, these upgrades will allow specimens to be exposed to much lower laser intensities, reducing photobleaching and photodamage, while providing a significant increase in resolution compared to the old system. In addition, images and multicolor 3D stacks can be collected much more rapidly in resonant scanning mode, while not sacrificing quality, meaning that experiments can be conducted quickly and efficiently, saving time and money for our users. The PMB Core has supported hundreds of peer-reviewed publications and dozens of grant applications over the years. The Core has an ongoing quest to constantly improve and upgrade Core facilities in support of its user base. This has been achieved using funds from NIH SIG applications as well as considerable institutional support from MGH leadership. Providing our Core users with continued access to up-to-date confocal imaging is an essential part of maintaining the MGH community at the cutting edge of NIH-funded, health-related research.
项目总结

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Dennis Brown其他文献

Dennis Brown的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Dennis Brown', 18)}}的其他基金

Cell Biology of Vasopressin-induced Water Channels-Research Supplement
加压素诱导的水通道的细胞生物学-研究补充
  • 批准号:
    10835229
  • 财政年份:
    2023
  • 资助金额:
    $ 29.61万
  • 项目类别:
Cell Biology Core
细胞生物学核心
  • 批准号:
    10586202
  • 财政年份:
    2023
  • 资助金额:
    $ 29.61万
  • 项目类别:
An Open-Labeled, Single Arm Phase 2 Efficacy and Safety Study of REM-001 Photodynamic Therapy (PDT) for Treatment of Cutaneous Metastatic Breast Cancer (CMBC)
REM-001 光动力疗法 (PDT) 治疗皮肤转移性乳腺癌 (CMBC) 的开放标记单臂 2 期疗效和安全性研究
  • 批准号:
    10699535
  • 财政年份:
    2023
  • 资助金额:
    $ 29.61万
  • 项目类别:
Defining protein:protein interactions for the regulation of renal V-ATPase function: role in expression, assembly and trafficking.
定义蛋白质:调节肾 V-ATP 酶功能的蛋白质相互作用:在表达、组装和运输中的作用。
  • 批准号:
    10670311
  • 财政年份:
    2019
  • 资助金额:
    $ 29.61万
  • 项目类别:
Defining protein:protein interactions for the regulation of renal V-ATPase function: role in expression, assembly and trafficking.
定义蛋白质:调节肾 V-ATP 酶功能的蛋白质相互作用:在表达、组装和运输中的作用。
  • 批准号:
    10454931
  • 财政年份:
    2019
  • 资助金额:
    $ 29.61万
  • 项目类别:
Defining protein:protein interactions for the regulation of renal V-ATPase function: role in expression, assembly and trafficking.
定义蛋白质:调节肾 V-ATP 酶功能的蛋白质相互作用:在表达、组装和运输中的作用。
  • 批准号:
    10207619
  • 财政年份:
    2019
  • 资助金额:
    $ 29.61万
  • 项目类别:
A Zeiss LSM800 confocal microscope with Airyscan
配备 Airyscan 的 Zeiss LSM800 共焦显微镜
  • 批准号:
    9075249
  • 财政年份:
    2016
  • 资助金额:
    $ 29.61万
  • 项目类别:
Cell biology of vasopressin-induced water channels
加压素诱导的水通道的细胞生物学
  • 批准号:
    10005038
  • 财政年份:
    2012
  • 资助金额:
    $ 29.61万
  • 项目类别:
Cell Biology of Vasopressin-induced Water Channels
加压素诱导的水通道的细胞生物学
  • 批准号:
    10652774
  • 财政年份:
    2012
  • 资助金额:
    $ 29.61万
  • 项目类别:
Cell biology of vasopressin-induced water channels
加压素诱导的水通道的细胞生物学
  • 批准号:
    9176186
  • 财政年份:
    2012
  • 资助金额:
    $ 29.61万
  • 项目类别:

相似海外基金

REU Site: Design, Create, and Innovate 3-Dimensional User Interfaces to Improve Human Sensory and Motor Performance in Virtual Environments (HUMANS MOVE)
REU 网站:设计、创建和创新 3 维用户界面,以提高虚拟环境中的人类感官和运动表现 (HUMANS MOVE)
  • 批准号:
    2349771
  • 财政年份:
    2024
  • 资助金额:
    $ 29.61万
  • 项目类别:
    Standard Grant
CAREER: Atomic-level understanding of stability and transition kinetics of 3-dimensional interfaces under irradiation
职业:对辐照下 3 维界面的稳定性和转变动力学的原子水平理解
  • 批准号:
    2340085
  • 财政年份:
    2024
  • 资助金额:
    $ 29.61万
  • 项目类别:
    Continuing Grant
Artificial fabrication of 3-dimensional noncollinear magnetic order and magnetization manipulation by spin torque
三维非共线磁序的人工制造和自旋转矩磁化操纵
  • 批准号:
    23H00232
  • 财政年份:
    2023
  • 资助金额:
    $ 29.61万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Understanding of 3-dimensional seismic behavior of RC frame high-speed railway/highway viaducts using FE analysis
使用有限元分析了解 RC 框架高速铁路/公路高架桥的 3 维抗震性能
  • 批准号:
    23H01489
  • 财政年份:
    2023
  • 资助金额:
    $ 29.61万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Modernization of 3-dimensional printing capabilities at the Aquatic Germplasm and Genetic Resource Center
水产种质和遗传资源中心 3 维打印能力的现代化
  • 批准号:
    10736961
  • 财政年份:
    2023
  • 资助金额:
    $ 29.61万
  • 项目类别:
The 3-dimensional nest of the honey bee: organization, development, and impact on colony function
蜜蜂的 3 维巢穴:组织、发育及其对蜂群功能的影响
  • 批准号:
    2216835
  • 财政年份:
    2023
  • 资助金额:
    $ 29.61万
  • 项目类别:
    Standard Grant
Research on high-density 3-dimensional polymer optical waveguide device for photonics-electronics convergence
光电子融合高密度三维聚合物光波导器件研究
  • 批准号:
    23H01882
  • 财政年份:
    2023
  • 资助金额:
    $ 29.61万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Scaff-Net: 3 Dimensional multiphoton polymerisation printed scaffolds for medium throughput recording from stem cell derived human cortical networks.
Scaff-Net:3 维多光子聚合打印支架,用于从干细胞衍生的人类皮质网络进行中等通量记录。
  • 批准号:
    EP/X018385/1
  • 财政年份:
    2023
  • 资助金额:
    $ 29.61万
  • 项目类别:
    Research Grant
3-dimensional prompt gamma imaging for online proton beam dose verification
用于在线质子束剂量验证的 3 维瞬发伽马成像
  • 批准号:
    10635210
  • 财政年份:
    2023
  • 资助金额:
    $ 29.61万
  • 项目类别:
Equipment: MRI: Track 1 Acquisition of a 3-Dimensional Nanolithography Instrument
设备:MRI:轨道 1 获取 3 维纳米光刻仪器
  • 批准号:
    2320636
  • 财政年份:
    2023
  • 资助金额:
    $ 29.61万
  • 项目类别:
    Standard Grant
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了