Label-free optical imaging for human mesoscale connectivity with a focus on deep brain stimulation targets

用于人体中尺度连接的无标记光学成像，重点关注深部脑刺激目标

• 批准号: 10586107
• 负责人: TANER AKKIN
• 金额: $ 49.71万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-03-07 至 2027-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10586107
• 关键词: 3-Dimensional; Algorithms; Anatomy; Animal Model; Architecture; Atlases; Axon; Birefringence; Brain; Brain Diseases; Brain Mapping; Brain imaging; Cells; Deep Brain Stimulation; Development; Diffusion Magnetic Resonance Imaging; Disease; Dissection; Electroencephalography; Electron Microscopy; Face; Fascicle; Fiber; Foundations; Functional Magnetic Resonance Imaging; Future; Generations; Goals; Histologic; Human; Image; Imaging technology; Injections; Internal Capsule; Label; Lasers; Lateral; Location; Macaca mulatta; Maps; Mental disorders; Methods; Microscopic; Motor; Motor Cortex; Neurons; Optical Coherence Tomography; Optics; Pathway interactions; Pattern; Performance; Phase; Population; Rattus; Resolution; Sampling; Structure; Structure of subthalamic nucleus; Synapses; System; Techniques; Technology; Thalamic structure; Tissues; Tracer; Visible Radiation; Visualization; Work; analytical tool; attenuation; brain circuitry; brain tissue; cingulate cortex; connectome; density; falls; gray matter; human imaging; image processing; imaging modality; improved; in vivo; meter; multiparametric imaging; nanoscale; nervous system disorder; neural tract; neuroimaging; neurosurgery; nonhuman primate; novel; optical imaging; polarized light; quantitative imaging; reconstruction; targeted treatment; tractography; white matter

PROJECT SUMMARY AND ABSTRACT Many neurological and psychiatric disorders are essentially connectionist disorders: certain sets of neurons have abnormally increased or decreased connectivity with other sets of neurons. Deep brain stimulation (DBS) therapies target small, unique populations of axons and/or cell bodies in order to treat brain disorders and normalize connectivity. Thus, mapping the wiring diagram of the brain is an important goal. Macroscale connectivity has been studied indirectly in humans using noninvasive neuroimaging. In order to develop a much higher resolution connectivity map of the brain, this project will develop depth-resolved polarized light imaging to visualize axons and fiber tracts. Since brain imaging and mapping at microscopic resolution is feasible with intrinsic optical contrasts (e.g. polarization-based) and depth-resolved block-face imaging is desired before histological processing, we have developed the serial optical coherence scanner (SOCS) for large-scale or whole brain imaging with microscopic resolution. SOCS combines a polarization-maintaining fiber based polarization-sensitive optical coherence tomography and a tissue slicer. This project will create a novel SOCS system that can image axonal tracts at the micron scale spatial resolution using unbiased optical contrasts (Aim 1). The approach will be evaluated, refined, and compared in the same brain tissue to neural tract-tracer labeling of tracts associated with DBS targets for brain disorders, in nonhuman animal models (Aim 2). The approach will then be applied to DBS targets in the human brain (Aim 3). The physical scales at which this project investigates the brain microstructure are unique (1-10 μm resolution across centimeters of tissue). This project will pave the way for the foundation of a future human connectome at the micron scale, which is the highest resolution achievable with current optical technology for imaging an entire human brain.

项目总结与摘要