Physiology and Behavior Core

生理学和行为核心

• 批准号: 10588975
• 负责人: Jill Lynn Silverman
• 金额: $ 23.82万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-02-01 至 2028-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10588975
• 关键词: Address; Affective; Animals; Anxiety; Apnea; Applications Grants; Area; Attention; Behavior; Behavioral; Biological; Biological Assay; Biomedical Research; Breeding; Cardiovascular Physiology; Chronic; Chronic Kidney Failure; Client; Clinical Trials; Cognition; Communities; Complement; Complex; Consultations; Data; Degenerative polyarthritis; Dementia; Development; Diabetes Mellitus; Disease; Ensure; Equilibrium; Event; Exercise; Female; Gait; Generations; Genetic; Genotype; Health; Health system; Heart Diseases; Heterogeneity; Human; Hyperglycemia; Hypertension; Image; Immune; Inflammation; Insulin Resistance; Laboratories; Learning; Memory; Mental Depression; Metabolic; Metabolic Diseases; Metabolic syndrome; Metabolism; Mission; Monitor; Motor; Mus; Neurologic Symptoms; Neuropathy; Nociception; Obesity; Outcome Measure; Pain; Participant; Patients; Phenotype; Physiological; Physiology; Procedures; Publications; Recommendation; Renal function; Reproducibility; Research; Research Design; Research Personnel; Resources; Respiratory physiology; Retinal Diseases; Risk Factors; Scientist; Services; Sleep; Sleep Deprivation; Sleep Stages; Social Behavior; Specialist; Technology; Testing; Training; behavior test; behavioral phenotyping; body system; cancer type; cohort; energy balance; executive function; flexibility; gastrointestinal function; in vivo; in vivo evaluation; innovation; intestinal barrier; male; metabolic phenotype; motor disorder; mouse model; mutant; neurophysiology; non-alcoholic fatty liver disease; pre-clinical; pre-clinical research; programs; sex; sexual dimorphism; tool; training opportunity; wound healing

ABSTRACT—PHYSIOLOGY AND BEHAVIOR (PB) CORE Numerous physiological and behavioral causes, complications and consequences of diabetes and obesity can be assessed in mouse models. Key physiological and behavioral phenotypes in mice are providing useful outcome measures and translational markers to inform the development of new treatments for these and other metabolic conditions in humans. The mission of the Physiology and Behavior (PB) Core will be to advance the understanding of behavioral manifestations and pathophysiological mechanisms and systemic consequences of disease. A selection of unique, complex, and innovative in vivo tests and procedures will be combined with consultative services on planning, analyzing, and interpretation of experimental results and outcomes measures in living mice. PB Core services will complement those of other Cores in the national MPMOD Consortium and the MMH and Animal Cores of the Center. The PB Core will also leverage the expertise and capabilities of several laboratories, cores, and service centers at UC Davis to provide ancillary analytical services and resources in support of investigators using the Center for studies on live mice. The PB Core will be service-oriented, client-facing, and research-supportive. The PB Core will be staffed by a Leader, co- Leader, Core Coordinators, Technical Specialists, and 5 Scientific Consultants. The mission of the PB Core will be accomplished through the following 2 Specific Aims: Specific Aim 1: Provide innovative, comprehensive, high quality, state-of-the art physiological and behavioral testing in vivo of obesity and diabetes phenotypes to investigators from across the biomedical community. Specific Aim 2: Provide clients with consultation services and advice on study design, test selection, and experimental planning, analysis and interpretation of results and outcomes measures, in the areas of physiology and behavior related to diabetes, obesity, and other metabolic disorders. Under these specific aims, the PB Core will carry out the following activities: • Providing comprehensive phenotyping tools for in vivo characterization of ocular, cardiovascular, kidney, and gastrointestinal functions in mouse models of obesity and diabetes in both sexes of mice; • Offering a battery of tests probing multiple behavioral domains (general health, motor capabilities, anxiety, affective and social behaviors, and memory and learning) in mice to understand how obesity and metabolic disorders influence neurophysiological and executive functions; • Conducting analyses of exercise, gait, sleeping stages, spindle events, and apneas during sleep by respiratory physiology paired with neurophysiology, which will provide essential information for studying alterations of energy balance in mice and complement services provided by the MMH Core; • Providing unique, innovative services to assess special physiological functions impacted by diabetes and/or obesity, including intestinal barrier function, nociception, imaging, and neuropathy in mice; • Offering state-of-the-art technologies to observe and measure outcomes in projects that pair behavioral with neurophysiology studies simultaneously in vivo; • Assisting investigators to generate high-quality and reproducible research results for publications, pilot data for grant proposals, and preclinical findings to inform human clinical trials; • Being flexible and responsive to requests for new and customizable tests and procedures; • Participating in the Vibrant Program to assist and train a new generation of scientists with a tailored focus toward underrepresented investigators and increasing diversity in biomedical research. Infused in all activities of the PB Core will be necessary controls, tests conducted in replicates, appropriate group sizes, use males and females to address sex as a biological variable, and other aspects consistent with the principles of rigor and reproducibility.

抽象-生理和行为(PB)核心 糖尿病和肥胖症的许多生理和行为原因、并发症和后果 在小鼠模型中进行评估。小鼠的关键生理和行为表型提供了有用的 结果衡量标准和翻译标记，为这些和其他疾病的新治疗方法的开发提供信息 人类的新陈代谢状况。生理和行为(PB)核心的使命将是推动 对行为表现、病理生理机制和系统后果的理解 疾病的威胁。一系列独特、复杂和创新的体内测试和程序将与 关于实验结果和结果的规划、分析和解释的咨询服务 在活体小鼠身上进行测量。PB核心服务将补充国家MPMOD中其他核心的服务 财团和该中心的MMH和动物核心。PB Core还将利用专业知识和 加州大学戴维斯分校的几个实验室、核心和服务中心提供辅助分析的能力 支持使用活鼠研究中心的研究人员的服务和资源。PB Core将 以服务为导向，面向客户，支持研究。PB核心将由一名领导、联合- 领导、核心协调员、技术专家和5名科学顾问。 PB核心的使命将通过以下两个具体目标来完成： 具体目标1：提供创新、全面、高质量、最先进的生理和 向来自世界各地的研究人员提供肥胖和糖尿病表型的活体行为测试 生物医学社区。 具体目标二：为客户提供研究设计、测试选择、 以及试验性规划、分析和解释这些领域的成果和成果措施 与糖尿病、肥胖症和其他代谢紊乱有关的生理和行为。 根据这些具体目标，方案规划核心将开展以下活动： ·提供全面的表型分析工具，用于眼部、心血管、 肥胖和糖尿病模型小鼠的肾脏和胃肠功能； ·提供一系列检测多个行为领域的测试(一般健康、运动能力、 焦虑、情感和社交行为，以及记忆和学习)，以了解肥胖是如何 代谢紊乱会影响神经生理和执行功能； ·通过以下方式对睡眠中的运动、步态、睡眠阶段、纺锤事件和呼吸暂停进行分析 呼吸生理学与神经生理学相结合，这将为 研究小鼠能量平衡的变化和MMH核心提供的补充服务； ·提供独特的创新服务，以评估受糖尿病影响的特殊生理功能 和/或肥胖，包括小鼠的肠道屏障功能、伤害性感觉、成像和神经病变； ·提供最先进的技术来观察和衡量项目的结果 在活体内同时进行行为和神经生理学研究； ·协助调查人员为出版物产生高质量和可复制的研究结果， 拨款建议的试点数据，以及为人类临床试验提供信息的临床前研究结果； ·灵活应对对新的和可定制的测试和程序的要求； ·参与活力计划，以量身定做的方式协助和培训新一代科学家 将重点放在代表性不足的研究人员上，并增加生物医学研究的多样性。 在PB核心的所有活动中注入必要的控制、在重复中进行的测试、适当 群体规模，使用男性和女性来解决性别作为生物变量，以及其他方面与 严谨和可重复性的原则。