Physiology and Behavior Core

生理学和行为核心

• 批准号: 10588975
• 负责人: Jill Lynn Silverman
• 金额: $ 23.82万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-02-01 至 2028-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10588975
• 关键词: Address; Affective; Animals; Anxiety; Apnea; Applications Grants; Area; Attention; Behavior; Behavioral; Biological; Biological Assay; Biomedical Research; Breeding; Cardiovascular Physiology; Chronic; Chronic Kidney Failure; Client; Clinical Trials; Cognition; Communities; Complement; Complex; Consultations; Data; Degenerative polyarthritis; Dementia; Development; Diabetes Mellitus; Disease; Ensure; Equilibrium; Event; Exercise; Female; Gait; Generations; Genetic; Genotype; Health; Health system; Heart Diseases; Heterogeneity; Human; Hyperglycemia; Hypertension; Image; Immune; Inflammation; Insulin Resistance; Laboratories; Learning; Memory; Mental Depression; Metabolic; Metabolic Diseases; Metabolic syndrome; Metabolism; Mission; Monitor; Motor; Mus; Neurologic Symptoms; Neuropathy; Nociception; Obesity; Outcome Measure; Pain; Participant; Patients; Phenotype; Physiological; Physiology; Procedures; Publications; Recommendation; Renal function; Reproducibility; Research; Research Design; Research Personnel; Resources; Respiratory physiology; Retinal Diseases; Risk Factors; Scientist; Services; Sleep; Sleep Deprivation; Sleep Stages; Social Behavior; Specialist; Technology; Testing; Training; behavior test; behavioral phenotyping; body system; cancer type; cohort; energy balance; executive function; flexibility; gastrointestinal function; in vivo; in vivo evaluation; innovation; intestinal barrier; male; metabolic phenotype; motor disorder; mouse model; mutant; neurophysiology; non-alcoholic fatty liver disease; pre-clinical; pre-clinical research; programs; sex; sexual dimorphism; tool; training opportunity; wound healing

ABSTRACT—PHYSIOLOGY AND BEHAVIOR (PB) CORE Numerous physiological and behavioral causes, complications and consequences of diabetes and obesity can be assessed in mouse models. Key physiological and behavioral phenotypes in mice are providing useful outcome measures and translational markers to inform the development of new treatments for these and other metabolic conditions in humans. The mission of the Physiology and Behavior (PB) Core will be to advance the understanding of behavioral manifestations and pathophysiological mechanisms and systemic consequences of disease. A selection of unique, complex, and innovative in vivo tests and procedures will be combined with consultative services on planning, analyzing, and interpretation of experimental results and outcomes measures in living mice. PB Core services will complement those of other Cores in the national MPMOD Consortium and the MMH and Animal Cores of the Center. The PB Core will also leverage the expertise and capabilities of several laboratories, cores, and service centers at UC Davis to provide ancillary analytical services and resources in support of investigators using the Center for studies on live mice. The PB Core will be service-oriented, client-facing, and research-supportive. The PB Core will be staffed by a Leader, co- Leader, Core Coordinators, Technical Specialists, and 5 Scientific Consultants. The mission of the PB Core will be accomplished through the following 2 Specific Aims: Specific Aim 1: Provide innovative, comprehensive, high quality, state-of-the art physiological and behavioral testing in vivo of obesity and diabetes phenotypes to investigators from across the biomedical community. Specific Aim 2: Provide clients with consultation services and advice on study design, test selection, and experimental planning, analysis and interpretation of results and outcomes measures, in the areas of physiology and behavior related to diabetes, obesity, and other metabolic disorders. Under these specific aims, the PB Core will carry out the following activities: • Providing comprehensive phenotyping tools for in vivo characterization of ocular, cardiovascular, kidney, and gastrointestinal functions in mouse models of obesity and diabetes in both sexes of mice; • Offering a battery of tests probing multiple behavioral domains (general health, motor capabilities, anxiety, affective and social behaviors, and memory and learning) in mice to understand how obesity and metabolic disorders influence neurophysiological and executive functions; • Conducting analyses of exercise, gait, sleeping stages, spindle events, and apneas during sleep by respiratory physiology paired with neurophysiology, which will provide essential information for studying alterations of energy balance in mice and complement services provided by the MMH Core; • Providing unique, innovative services to assess special physiological functions impacted by diabetes and/or obesity, including intestinal barrier function, nociception, imaging, and neuropathy in mice; • Offering state-of-the-art technologies to observe and measure outcomes in projects that pair behavioral with neurophysiology studies simultaneously in vivo; • Assisting investigators to generate high-quality and reproducible research results for publications, pilot data for grant proposals, and preclinical findings to inform human clinical trials; • Being flexible and responsive to requests for new and customizable tests and procedures; • Participating in the Vibrant Program to assist and train a new generation of scientists with a tailored focus toward underrepresented investigators and increasing diversity in biomedical research. Infused in all activities of the PB Core will be necessary controls, tests conducted in replicates, appropriate group sizes, use males and females to address sex as a biological variable, and other aspects consistent with the principles of rigor and reproducibility.

摘要 - 生理学和行为（PB）核心 糖尿病和肥胖的许多身体和行为原因，并发症以及后果可以 在鼠标模型中进行评估。小鼠的关键生理和行为表型正在提供有用 结果措施和翻译标记，以告知这些和其他新疗法的开发 人类的代谢条件。生理和行为（PB）核心的使命将是促进 了解行为表现和病理生理机制和全身后果 疾病。各种独特，复杂和创新的体内测试和程序将与 有关实验结果和结果的计划，分析和解释的咨询服务 活小鼠的措施。 PB核心服务将完成国家MPMOD的其他核心 联盟和中心的MMH和动物核心。 PB核心还将利用专业知识和 加州大学戴维斯分校的几个实验室，核心和服务中心的功能可提供辅助分析 使用现场小鼠研究中心支持调查人员的服务和资源。 PB核心将 以服务为导向，面向客户和研究支持。 PB核心将由领导者组成 领导者，核心协调员，技术专家和5位科学顾问。 PB核心的任务将通过以下两个具体目标完成： 特定目标1：提供创新，全面，高质量，最先进的生理和 肥胖和糖尿病表型的行为测试向整个研究人员 生物医学界。 特定目标2：为客户提供有关研究设计，测试选择的建议， 以及在该领域的实验计划，分析和解释结果和结果措施 与糖尿病，肥胖和其他代谢障碍有关的生理和行为。 在这些特定目标下，PB核心将进行以下活动： •提供全面的表型工具，用于眼部，心血管的体内表征 肾脏和肥胖和糖尿病模型的胃肠道功能在两种小鼠的性别中； •提供一系列测试，以探测多个行为域（一般健康，运动能力， 小鼠的焦虑，情感和社交行为以及记忆和学习）以了解肥胖 代谢疾病影响神经生理和执行功能； •通过睡眠期间进行运动，步态，睡眠阶段，主轴事件和呼吸暂停的分析 呼吸生理与神经生理学配对，这将为 研究MMH核心提供的小鼠和补充服务的能量平衡的变化； •提供独特的创新服务，以评估受糖尿病影响的特殊生理功能 和/或肥胖，包括小鼠的肠屏障功能，伤害感受，成像和神经病； •提供最先进的技术来观察和衡量成对的项目的结果 神经生理学研究的行为仅在体内； •协助研究人员为出版物生成高质量和可重复的研究结果， 授予建议的试点数据和临床前发现，以告知人类临床试验； •灵活并响应对新的和可自定义的测试和程序的要求； •参加充满活力的计划，以协助和培训新一代科学家，并量身定制 专注于代表性不足的研究人员，并增加了生物医学研究的多样性。 注入PB核心的所有活动都将是必要的控制，重复进行的测试，适当 群体大小，使用男性和女性将性作为生物学变量，以及其他方面一致 严格和可重复性的原则。