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Lateral Hypothalamic Circuits for Feeding and Reinforcement

用于喂养和强化的外侧下丘脑回路

基本信息

批准号：
10588126
负责人：
Garret D Stuber
金额：
$ 45.81万
依托单位：
UNIVERSITY OF WASHINGTON
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-05-01 至 2026-02-28
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10588126
关键词：
Anatomy Animal Feed Animals Appetitive Behavior Architecture Area Behavioral Brain Brain region Calcium Cell physiology Cells Code Communities Consummatory Behavior Consumption Data Data Set Desire for food Disease Dynorphins Electric Stimulation Elements Fasting Food Foundations Funding Future Gene Expression Gene Expression Profile Gene Expression Profiling Genes Genetic Genetic Transcription Glutamates Goals Habenula Head Hour Hypothalamic structure Image Individual Lateral Lateral Hypothalamic Area Link Mental disorders Modification Molecular Molecular Profiling Monitor Motivation Mus Neuroanatomy Neurons Neuropeptides Neurotensin Organism Output Pathway interactions Pharmacology Study Play Population Process Psychological reinforcement Research Resources Response to stimulus physiology Rewards Rodent Role Satiation Seminal Shapes Signal Transduction Site Somatostatin Stimulus Sucrose Supporting Cell Surveys Testing Thalamic structure Therapeutic Intervention Ventral Tegmental Area Ventricular Viral Work addiction brain circuitry cell type data resource differential expression drug of abuse experimental study feeding insight interdisciplinary approach motivated behavior neural circuit neuropsychiatric disorder novel optogenetics parabrachial nucleus reward processing single-cell RNA sequencing temporal measurement tool two-photon

项目摘要

Abstract: Motivated behaviors such as feeding and reward-seeking are critical for an organism's survival. These processes require distributed neuronal networks and supporting cell types in multiple brain regions to be tightly regulated and tuned in order to orchestrate behavioral output. The lateral hypothalamic area (LHA) has long been identified as a critical neuroanatomical substrate for motivated behavior. Despite decades of research, the molecular identity of defined LHA cell types remains poorly understood. Additionally, while LHA neurons have been previously shown to encode appetitive and consummatory behaviors via distinct cellular ensembles, it remains unknown how distinct LHA output neurons contribute to these processes. Here we propose to continue to study the neural circuits of the LHA, and to identify how distinct cell types contribute to feeding and reinforcement. Using an interdisciplinary approach to leverage cutting edge tools such as single cell transcriptional profiling, two photon calcium imaging, and viral we aim to undercover key circuit elements, and novel circuit specific gene expression patterns that can be leveraged for future therapeutic interventions for addiction and other neuropsychiatric disorders.

摘要：有动机的行为，如觅食和寻求奖励，对有机体的生存至关重要。这些这一过程要求分布在多个脑区的神经元网络和支持细胞类型紧密相连为了协调行为输出而进行调节和调整。下丘脑外侧区(LHA)有较长的被确认为动机行为的关键神经解剖学底物。尽管进行了数十年的研究，已定义的LHA细胞类型的分子同一性仍然知之甚少。此外，虽然LHA神经元以前已经被证明通过不同的细胞集合来编码食欲和消耗性行为，目前尚不清楚LHA输出神经元对这些过程有何不同的贡献。在此，我们建议继续研究LHA的神经回路，并确定不同类型的细胞如何对摄食和增援。使用跨学科方法来利用尖端工具，如Single CELL 转录图谱、双光子钙成像和病毒我们的目标是隐藏关键的电路元件，以及新的电路特异性基因表达模式，可用于未来的治疗干预成瘾和其他神经精神障碍。