Nanobody-Based Electrochemical Biosensor for Real-Time Detection of Aerosolized SARS-CoV2

基于纳米抗体的电化学生物传感器,用于实时检测气溶胶 SARS-CoV2

基本信息

  • 批准号:
    10264330
  • 负责人:
  • 金额:
    $ 43.33万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2020
  • 资助国家:
    美国
  • 起止时间:
    2020-12-21 至 2022-11-30
  • 项目状态:
    已结题

项目摘要

ABSTRACT/PROJECT SUMMARY Coronavirus 2019 (COVID-19) has afflicted 6.2 million Americans and killed 190,000 as of early September 2020 (WHO website); a roughly 3% mortality. Between a shortage in testing and unidentified asymptomatic individuals, the actual number of those infected could be 6 to 24-fold higher than that reported. SARS-CoV-2 (CoV-2), the virus underlying the disease, results in a range of symptoms; in select cases a severe respiratory illness that impedes breathing that could lead to hospitalization and death. CoV-2 is transmitted person-to- person via inhalation of the virus through mucosal membranes of the nose and throat from transfer after touching a contaminated surface or by inhaling aerosolized virus. Unfortunately, COVID-19 is likely to be prevalent well into 2021 and beyond. We must increase our ability to test for CoV-2. First, testing is needed to diagnose individuals that are symptomatic or asymptomatic to reduce community spread. And second, monitoring gathering areas for airborne virus that could inform the decision to shutdown a space or implement disinfection and mitigation of an area. We propose to use an electrochemical biosensor in two detection devices, 1) a diagnostic breathalyzer for instant detection of CoV-2 and 2) an airborne detector for real-time, continuous surveillance of a large space. We have developed a novel ultra-sensitive, antibody-based electrochemical biosensor to detect CoV-2 repeat binding domain (RBD) spike protein. The technology is based on a micro-immunoelectrode (MIE) biosensor pioneered by the Cirrito laboratory to study protein dynamics in the setting of neurodegeneration (2,3). The biosensor uses voltammetry to measure the oxidation of tyrosine amino acids; oxidation is the release of electrons that the biosensor measures as a change in current. Antibodies are covalently attached to the electrode surface to provide selectivity. Our prototype CoV-2 biosensor is sensitive to 2 femtogram/ml, compared to several current CoV-2 antigen tests that are sensitive to the low picogram/ml range. The proposal will first (Aim 1) optimize our CoV-2 biosensor to detect CoV-2 viral particles, as well as test several parameters to increase sensitivity and longevity. Aim 2 will build a test breathalyzer that will utilize a nebulizer to generate virus laden air containing aerosol droplets similar to a breath that contain defined concentrations of CoV-2 viral particles. Aim 3 will test the airborne biosensor in a realistic environment. Co-I Chakrabarty’s laboratory has unique capabilities of mimicking real-world environmental conditions, especially in the context of atmospheric aerosols, necessary for testing and optimizing the biosensor’s performance for field deployment. Atmospheric conditions include relative humidity (RH) and temperature, as well as common airborne pollutants found indoors. Finding novel means to detect the CoV-2, as well as create a platform to detect other and future pathogens, would enable us to limit the viral spread throughout the community in the current and future pandemics.
抽象/项目总结

项目成果

期刊论文数量(0)
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John R Cirrito其他文献

John R Cirrito的其他文献

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{{ truncateString('John R Cirrito', 18)}}的其他基金

The convergence of stress and sex on Abeta and tau metabolism and pathology
压力和性对 Abeta 和 tau 代谢及病理学的影响
  • 批准号:
    10734280
  • 财政年份:
    2023
  • 资助金额:
    $ 43.33万
  • 项目类别:
Nanobody-based electrochemical biosensor for real-time detection of aerosolized SARS-CoV2
基于纳米抗体的电化学生物传感器,用于实时检测雾化的 SARS-CoV2
  • 批准号:
    10656047
  • 财政年份:
    2022
  • 资助金额:
    $ 43.33万
  • 项目类别:
Nanobody-Based Electrochemical Biosensor for Real-Time Detection of Aerosolized SARS-CoV2
基于纳米抗体的电化学生物传感器,用于实时检测气溶胶 SARS-CoV2
  • 批准号:
    10320998
  • 财政年份:
    2020
  • 资助金额:
    $ 43.33万
  • 项目类别:
Effects of ApoE-enhancing Compounds on Alzheimers Disease Phenotypes In Vivo
ApoE 增强化合物对体内阿尔茨海默病表型的影响
  • 批准号:
    9752688
  • 财政年份:
    2018
  • 资助金额:
    $ 43.33万
  • 项目类别:
TEMPORAL RELATIONSHIP BETWEEN SYNAPTIC ACTIVITY AND ABETA AGGREGATION
突触活动与 ABETA 聚合之间的时间关系
  • 批准号:
    8699656
  • 财政年份:
    2013
  • 资助金额:
    $ 43.33万
  • 项目类别:
TEMPORAL RELATIONSHIP BETWEEN SYNAPTIC ACTIVITY AND ABETA AGGREGATION
突触活动与 ABETA 聚合之间的时间关系
  • 批准号:
    8566773
  • 财政年份:
    2013
  • 资助金额:
    $ 43.33万
  • 项目类别:
SYNAPTIC REGULATION OF ERK-MEDIATED AMYLOID-BETA METABOLISM
ERK 介导的淀粉样蛋白代谢的突触调节
  • 批准号:
    8517548
  • 财政年份:
    2012
  • 资助金额:
    $ 43.33万
  • 项目类别:
SYNAPTIC REGULATION OF ERK-MEDIATED AMYLOID-BETA METABOLISM
ERK 介导的淀粉样蛋白代谢的突触调节
  • 批准号:
    9064726
  • 财政年份:
    2012
  • 资助金额:
    $ 43.33万
  • 项目类别:
SYNAPTIC REGULATION OF ERK-MEDIATED AMYLOID-BETA METABOLISM
ERK 介导的淀粉样蛋白代谢的突触调节
  • 批准号:
    8342633
  • 财政年份:
    2012
  • 资助金额:
    $ 43.33万
  • 项目类别:
SYNAPTIC REGULATION OF ERK-MEDIATED AMYLOID-BETA METABOLISM
ERK 介导的淀粉样蛋白代谢的突触调节
  • 批准号:
    8661672
  • 财政年份:
    2012
  • 资助金额:
    $ 43.33万
  • 项目类别:

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