Utilizing beige fat to improve muscle function after rotator cuff repair

利用米色脂肪改善肩袖修复后的肌肉功能

• 批准号: 10605678
• 负责人: Brian Feeley
• 金额: $ 29.35万
• 依托单位: NORTHERN CALIFORNIA INSTITUTE/RES/EDU
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10605678
• 关键词: Adopted; Affect; American; Arthralgia; Arthritis; Award; Back Pain; Biological Assay; Biomedical Research; Cellular biology; Chronic; Clinical; Clinical Research; Communities; Data; Disease; Exposure to; Fatty acid glycerol esters; Funding; Future; Gene Expression; Goals; Grant; Human; Immersion; Improve Access; Individual; Injury; K-Series Research Career Programs; Laboratories; Latinx; Learning; Mentors; Mentorship; Metabolic; Mitochondria; Modeling; Muscle; Muscle function; Muscular Dystrophies; Musculoskeletal; Musculoskeletal Diseases; Organ; Orthopedic Surgery; Orthopedics; Osteoporosis; Outcome; Persons; Phase; Phenotype; Postdoctoral Fellow; Process; Research; Rotator Cuff; Science; Scientist; Source; Surgeon; System; Time; Tissues; Training; Translational Research; Underrepresented Populations; Underrepresented Students; United States; United States National Institutes of Health; Vertebral column; Woman; Work; advanced analytics; aging population; career; disability; improved; improved outcome; injury and repair; insight; laboratory experience; lost earning; medical specialties; multidisciplinary; muscle degeneration; muscle regeneration; next generation; post-doctoral training; programs; repaired; rotator cuff injury; sarcopenia; skills; stem cells; student training; success; transcriptomics; translational potential; undergraduate student

Project Summary The 2nd leading cause of disability worldwide is musculoskeletal disease, including back pain, arthritis, and osteoporosis. Musuloskeletal conditions disproportionately affect women and non-white people, a problem exacerbated by the fact that orthopedics is the least diverse clinical and biomedical research specialty. Musculoskeletal science represents an ideal multi-disciplinary scientific field to train students across the spectrum of transcriptomics, cellular biology, inter-organ cross talk, and translational research. Critical gaps in the current system are the fact that there is a lack of underrepresented trainees with the expertise to achieve NIH funding in orthopedic surgery, and a similar lack of clear mentorship and guidance for surgeon scientists to train the next generation of scientists that can ameliorate the burden of musculoskeletal disease on our aging population. While many surgeon scientists seek to train underrepresented applicants, most lack the framework and training necessary for the effective mentorship of underrepresented students in the fields of biomedical research, musculoskeletal disease, and orthopedic surgery. Bridging these gaps is a necessary and achievable goal to promote a diverse and equitable scientific community focused in improving the outcomes of orthopedic and musculoskeletal degenerative processes. This proposal will seek to bridge two critical gaps in our ability to train underrepresented people in the field of musculoskeletal biomedical research. In Aim 1, we will continue the immersive training of Steven Garcia towards a K08 award, providing him the necessary bridge towards independent funding. This will be achieved by allowing him to continue his post-doctoral work on the transcriptomic profiling of FAPs in muscle injury states as well as develop new insights leveraging spatial transcriptomic analysis of FAP gene expression. In Aim 2, we will enhance the mentorship program provided by the Feeley- Liu lab by providing mentorship training for Dr. Feeley through the well-established UCSF Diversity Mentorship program. Additionally, current and future underrepresented students will be given specific time within the laboratory training to learn advanced skills necessary (e.g. transcriptomic analysis, metabolic assays of mitochondrial function, advanced statistical analytics), as well as exposure to clinical orthopedic surgery to understand the translational potential of the current bench research.

项目摘要 全球第二大残疾原因是肌肉骨骼疾病，包括背部 疼痛关节炎和骨质疏松症肌肉骨骼疾病不成比例地影响 妇女和非白人，一个问题加剧了这一事实，整形外科是 临床和生物医学研究专业最少。肌肉骨骼科学 代表了一个理想的多学科科学领域，以培养学生跨越频谱 转录组学、细胞生物学、器官间串扰和转化研究。 目前系统的关键差距是， 具有专门知识的受训人员，以获得NIH在骨科手术方面的资助，以及类似的 缺乏对外科医生科学家培训下一代的明确指导和指导 科学家们可以减轻肌肉骨骼疾病对我们衰老的负担， 人口虽然许多外科医生科学家寻求培训代表性不足的申请人， 大多数国家缺乏有效指导所需的框架和培训， 生物医学研究、肌肉骨骼疾病、 和整形外科。弥合这些差距是一个必要和可实现的目标， 促进多样化和公平的科学界，专注于改善成果 骨科和肌肉骨骼退化的过程。 这项建议将寻求弥补我们在培训代表性不足的能力方面的两个关键差距 肌肉骨骼生物医学研究领域的人。在目标1中，我们将继续 史蒂芬·加西亚的沉浸式培训，为他提供必要的K 08奖项， 走向独立融资的桥梁。这将通过允许他继续 他在肌肉损伤状态下FAP转录组学分析的博士后工作， 利用FAP基因的空间转录组学分析， 表情在目标2中，我们将加强费利提供的导师计划- 刘实验室通过提供导师培训博士. Feeley通过完善的UCSF 多样性指导计划。此外，目前和未来的代表性不足的学生 将在实验室内给予特定的时间进行培训，以学习必要的高级技能 (e.g.转录组学分析，线粒体功能的代谢测定，高级 统计分析），以及接触临床骨科手术，以了解 目前台架研究的转化潜力。