Core B: Mouse Physiology and Surgery Core

核心 B：小鼠生理学和外科核心

• 批准号: 10612819
• 负责人: Erhe Gao
• 金额: $ 31.7万
• 依托单位: TEMPLE UNIV OF THE COMMONWEALTH
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-05-01 至 2025-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10612819
• 关键词: Animal Model; Animals; Area; Biochemical; Blood Pressure; Cardiac; Cardiorenal syndrome; Cardiovascular Physiology; Cardiovascular system; Catheterization; Catheters; Cell Therapy; Cells; Chronic; Collaborations; Conscious; Data; Data Analyses; Distant; Drug Delivery Systems; Echocardiography; Experimental Designs; Functional disorder; Gene Delivery; Genes; Goals; Heart; Heart Injuries; Heart failure; Histologic; Infrastructure; Injections; Intravenous; Left ventricular structure; Measurement; Mediating; Methodology; MicroRNAs; Modeling; Mus; Muscle Cells; Myocardial; Myocardial Infarction; Myocardial Ischemia; Operative Surgical Procedures; Organ; Osmosis; Pathology; Pathway interactions; Phenotype; Physiology; Program Research Project Grants; Protocols documentation; Pump; Reperfusion Injury; Reproducibility; Research Personnel; Resources; Services; Structure; Telemetry; Vertebral column; Viral; aorta constriction; cardioprotection; collaborative environment; coronary artery occlusion; exosome; experience; heart function; hemodynamics; implantation; in vivo; mouse model; myocardial injury; novel; pressure; programs; repair strategy; stem cell delivery; stem cells; success

Core B SUMMARY The Mouse Physiology and Surgical Core (Core B) will be a focal point of the program by providing the infrastructure to support numerous experimental approaches to assess cardiac physiology and pathology in the intact animal. Utilizing the vast experience and expertise of the Core Director's, and well established and proven protocols for the assessment of cardioprotection in multiple animal models. The Core will not only provide a consistent and reproducible service in support of the projects but will further participate in experimental design, prioritization of resources, and the interpretation of data. In addition, Core B will identify additional areas for collaboration across all projects as the program matures and thus be central to the interactive environment and comprehensive analysis of the overall platform as data is generated. The scientific core will provide a wide-range of services pertinent to the overall success of the program including in providing multiple models of myocardial injury to induce heart failure with also distant organ dysfunction such as cardiorenal syndrome. The core will also be a resource to assist in cell and gene delivery to the heart and also the assessment of cardiac function via echocardiography and catheter based hemodynamic measurement. Further, the core will be invaluable to assist PPG investigators in the assessment of cardiac structure by multiple methodologies, and histological and biochemical assessment of myocardial injury. These services will provide the backbone for all Projects of this Program Project Grant to achieve their goals more efficiently.

核心B总结 小鼠生理学和外科核心（核心 B）将成为该计划的焦点，通过提供 支持多种实验方法来评估心脏生理学和病理学的基础设施 完整的动物。利用核心董事的丰富经验和专业知识，以及成熟且经过验证的 在多种动物模型中评估心脏保护的方案。核心不仅提供 提供一致且可重复的服务来支持项目，但将进一步参与实验设计， 资源的优先级和数据的解释。此外，核心 B 将确定其他领域 随着项目的成熟，所有项目之间的协作将成为交互式环境的核心， 生成数据时对整个平台进行综合分析。科学核心将提供广泛的 与该计划总体成功相关的服务，包括提供多种心肌模型 损伤诱发心力衰竭以及远处器官功能障碍，例如心肾综合征。核心还将 是协助细胞和基因递送至心脏以及通过以下方式评估心脏功能的资源 超声心动图和基于导管的血流动力学测量。此外，核心对于协助 PPG 研究人员通过多种方法、组织学和组织学方法评估心脏结构 心肌损伤的生化评估。这些服务将为该项目的所有项目提供支柱 计划项目补助金以更有效地实现他们的目标。