Mouse Physiology/ Surgery Core

小鼠生理学/外科核心

• 批准号: 9052809
• 负责人: Erhe Gao
• 金额: $ 34.26万
• 依托单位: TEMPLE UNIV OF THE COMMONWEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9052809
• 关键词: Animal Model; Blood Pressure; Cardiac; Cardiovascular Physiology; Cardiovascular system; Catheterization; Chronic; Conscious; Coronary artery; Development; Drug Delivery Systems; Echocardiography; Gene Delivery; Heart; Heart Hypertrophy; Heart failure; Hypertrophy; Injection of therapeutic agent; Injury; Ischemia; Lead; Left ventricular structure; Ligation; Measurement; Measures; Mediating; Modeling; Mus; Myocardial; Myocardial Infarction; Myocardial Ischemia; Operative Surgical Procedures; Phenotype; Physiological; Physiology; Productivity; Pump; Reperfusion Injury; Reperfusion Therapy; Research Personnel; Services; Telemetry; Testing; Therapeutic; Venous; Viral; constriction; coronary artery occlusion; hemodynamics; implantation; in vivo; injury and repair; mouse model; novel; pressure; repaired; success; tool

This Core will provide high-fidelity measures of cardiac physiology for the various mouse lines described in the 3 Projects and provide models of cardiac injury for all Projects to study. Specifically, this Core Unit will be responsible for providing 1) mouse models of cardiac hypertrophy and ischemic injury where both models can lead to heart failure (HF), 2) complete and comprehensive cardiovascular physiology measurements (phenotyping) of all mouse models detailed through the PPG application, and 3) cardiac viral-mediated gene delivery. Surgical mouse models include coronary artery occlusion models for ischemia/reperfusion (l/R) injury or complete coronary artery ligation producing a myocardial infarction (Ml). Also, transverse aortic constriction (TAC) for models of hypertrophy and HF will be handled in this Core. The Core will also help investigators with surgical implantation of AzIet mini-osmotic pumps for chronic drug delivery. Further, cardiac viral-mediated gene delivery can be done either though IV injection or more invasively directly to the heart as seen in this application. Comprehensive mouse cardiovascular physiological phenotyping includes in-depth assessment of murine in vivo cardiac function via echocardiography and hemodynamic measurements via Millar catheterization. Further, this Core will assess conscious blood pressure and electric cardiogram (ECG) using telemetry. This Core will be utilized by all Projects and will continue to be instrumental in providing reproducible and high-quality mouse models of cardiac injury and physiological cardiovascular phenotyping.

该核心将为3个项目中描述的各种小鼠系提供高保真的心脏生理学测量，并为所有项目提供心脏损伤模型。具体来说，这个核心单元将负责提供1)心脏肥大和缺血性损伤的小鼠模型，这两种模型都可能导致心力衰竭（HF）， 2)完整和全面的心血管生理学测量