Mouse Physiology/ Surgery Core

小鼠生理学/外科核心

• 批准号: 8845233
• 负责人: Erhe Gao
• 金额: $ 33.76万
• 依托单位: TEMPLE UNIV OF THE COMMONWEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8845233
• 关键词: A Mouse; Animal Model; Blood Pressure; Cardiac; Cardiovascular Physiology; Cardiovascular system; Catheterization; Chronic; Conscious; Coronary artery; Development; Drug Delivery Systems; Echocardiography; Gene Delivery; Heart; Heart Hypertrophy; Heart failure; Hypertrophy; Injection of therapeutic agent; Injury; Ischemia; Lead; Left ventricular structure; Ligation; Measurement; Measures; Mediating; Modeling; Mus; Myocardial; Myocardial Infarction; Myocardial Ischemia; Operative Surgical Procedures; Phenotype; Physiological; Physiology; Productivity; Pump; Reperfusion Injury; Reperfusion Therapy; Research Personnel; Services; Telemetry; Testing; Therapeutic; Venous; Viral; constriction; coronary artery occlusion; hemodynamics; implantation; in vivo; injury and repair; mouse model; novel; pressure; repaired; success; tool

This Core will provide high-fidelity measures of cardiac physiology for the various mouse lines described in the 3 Projects and provide models of cardiac injury for all Projects to study. Specifically, this Core Unit will be responsible for providing 1) mouse models of cardiac hypertrophy and ischemic injury where both models can lead to heart failure (HF), 2) complete and comprehensive cardiovascular physiology measurements (phenotyping) of all mouse models detailed through the PPG application, and 3) cardiac viral-mediated gene delivery. Surgical mouse models include coronary artery occlusion models for ischemia/reperfusion (l/R) injury or complete coronary artery ligation producing a myocardial infarction (Ml). Also, transverse aortic constriction (TAC) for models of hypertrophy and HF will be handled in this Core. The Core will also help investigators with surgical implantation of AzIet mini-osmotic pumps for chronic drug delivery. Further, cardiac viral-mediated gene delivery can be done either though IV injection or more invasively directly to the heart as seen in this application. Comprehensive mouse cardiovascular physiological phenotyping includes in-depth assessment of murine in vivo cardiac function via echocardiography and hemodynamic measurements via Millar catheterization. Further, this Core will assess conscious blood pressure and electric cardiogram (ECG) using telemetry. This Core will be utilized by all Projects and will continue to be instrumental in providing reproducible and high-quality mouse models of cardiac injury and physiological cardiovascular phenotyping.

该核心将为3个项目中描述的各种小鼠品系提供心脏生理学的高保真测量，并为所有研究项目提供心脏损伤模型。具体而言，该核心单位将负责提供1）心脏肥大和缺血性损伤的小鼠模型，其中两种模型均可导致心力衰竭（HF），2）完整和全面的心血管生理学测量 通过PPG应用详细描述的所有小鼠模型的表型分析，和3）心脏病毒介导的基因递送。 手术小鼠模型包括缺血/再灌注（I/R）损伤的冠状动脉闭塞模型或产生心肌梗塞（MI）的完全冠状动脉结扎。此外，肥大和HF模型的横向主动脉缩窄（TAC）将在本核心中处理。核心还将帮助研究人员进行AzIet微型渗透泵的手术植入，用于慢性药物输送。此外，心脏病毒介导的基因递送可以通过IV注射或更具侵入性地直接向心脏进行，如本申请中所见。 全面的小鼠心血管生理表型分析包括通过超声心动图对小鼠体内心脏功能的深入评估和通过Millar导管插入术进行的血液动力学测量。 此外，该核心将使用遥测技术评估清醒血压和心电图（ECG）。该核心将被所有项目使用，并将继续有助于提供心脏损伤和生理心血管表型的可重现和高质量的小鼠模型。