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Human Skin Disease Resource-based Center

人类皮肤病资源中心

基本信息

批准号：
10615211
负责人：
Rachael Ann Clark
金额：
$ 88.28万
依托单位：
BRIGHAM AND WOMEN'S HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-07-19 至 2026-04-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10615211
关键词：
ATAC-seq Acceleration Address Advisory Committees Animal Model Animals Antibodies Biological Biopsy Blood Cells Clinical Trials Color Communication Communities Consent Cryopreservation Cytometry DNA Library Diagnosis Disease Eligibility Determination Environment Flow Cytometry Formalin Frozen Sections Funding Gene Expression Genetic Fingerprintings Genotype Goals Human Human Biology Image Immune system Immunodeficient Mouse Individual Institution International Knowledge Leadership Malignant Neoplasms Medicine Mentorship Modeling Mus Paraffin Embedding Pathologic Pathology Patients Physicians Plasma Population Positioning Attribute Protein Analysis Proteins Protocols documentation RNA Research Research Personnel Research Project Grants Resources Sampling Science Scientist Serum Services Shipping Skin Specimen System T cell receptor repertoire sequencing T-Cell Receptor Genes Talents Techniques Technology Time Tissue Embedding Tissues Training Underrepresented Populations Vaccination Woman Work biobank cellular imaging cost digital empowerment human tissue insight member methicillin resistant Staphylococcus aureus mouse model nano-string new technology next generation outreach people of color protein expression recruit single-cell RNA sequencing skin disorder spectrograph symposium therapy development tool translational scientist

项目摘要

Project Summary/Abstract: Overall Component Mouse models have provided many biologic insights and remain the most popular system in which to conduct skin disease research. However. there are significant differences between the skin and immune systems of mice and humans and these differences are incompletely characterized, making it difficult to know if observations made in mice will hold true in humans. Research carried out on human cells and tissues can address this knowledge gap. Human biobanks and powerful new analytic techniques have become available that make high-quality human skin disease research accessible. The goals of this Center are to accelerate human skin disease research by providing researchers at any institution with access to human specimens and cutting edge analytic techniques and to bring new diverse investigators into the field of human skin disease research. We include 45 research projects from investigators who wish to utilize Center services; however, any researcher wishing to carry out human skin disease research is a potential member of the research community. The Center is composed of an Administrative Core and three Resource Cores. The Administrative Core manages and oversees all activities of the Center and administers Diversity and Outreach activities, including funding for diverse investigators at multiple stages of training and the biennial International Conference on Human Skin Disease. The Human Tissues Biobank Core provides access to over 113,000 highly characterized consented patients, over 1.5 million banked pathologic specimens, both searchable by diagnosis, as well as to fresh human skin, purified cell populations from human skin and immunodeficient mice grafted with human skin and blood. The Single Cell and Immunoanalysis Core provides access to single cell RNA and ATAC sequencing, flow cytometry-based single cell imaging, mass cytometry by time of flight (CyTOF) and high throughput TCR sequencing. The Next Generation Tissue Analysis & Imaging Core provides access to six color tyramide amplification based immunostaining combined with spectral imaging and automated cell analysis, NanoString RNA and DNA profiling and Digital Spatial Profiling, a state-of-the-art technique that that can profile expression of 10-1000s of protein or RNA targets in FFPE and frozen sections. In summary, the Center provides access to biobanks and cutting-edge human analytic techniques that enable translational researchers to carry out high quality human skin disease research.

项目概要/摘要：整体组件小鼠模型提供了许多生物学见解，并且仍然是最受欢迎的系统，皮肤病研究然而.皮肤和免疫系统之间有显着差异，小鼠和人类，这些差异的特征不完全，很难知道，在小鼠身上进行的观察也适用于人类。对人体细胞和组织进行的研究可以填补这一知识空白。人类生物库和强大的新分析技术已经成为可能使高质量的人类皮肤病研究变得容易。该中心的目标是加快通过向任何机构的研究人员提供人体标本，尖端的分析技术，并将新的多样化研究人员带入人类皮肤病领域 research.我们包括希望利用中心服务的研究人员的45个研究项目;然而，希望进行人类皮肤病研究的研究人员是该研究的潜在成员社区该中心由一个行政核心和三个资源核心组成。行政核心管理和监督中心的所有活动，并管理多样性和推广活动，包括为处于多个培训阶段的各种调查人员提供资金，以及两年一次的国际人类皮肤病会议。人类组织生物库核心提供了超过113，000个高度特征化的知情同意患者，超过150万个库存病理标本，均可通过诊断，以及新鲜的人皮肤，来自人皮肤和免疫缺陷小鼠的纯化细胞群移植了人皮和血单细胞和免疫分析核心提供对单细胞的访问 RNA和ATAC测序、基于流式细胞术的单细胞成像、飞行时间质谱分析（CyTOF）和高通量TCR测序。下一代组织分析和成像核心提供了基于六色酪胺扩增的免疫染色与光谱成像的结合，自动化细胞分析，NanoString RNA和DNA分析以及数字空间分析，这是最先进的可以在FFPE和冷冻切片中分析10- 1000个蛋白质或RNA靶标表达技术。总而言之，该中心提供生物库和尖端的人类分析技术，翻译研究人员进行高质量的人类皮肤病研究。

项目成果

期刊论文数量（27）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

In-Depth Proteomic Map of Innate Lymphoid Cells from Healthy Human Skin and Blood.

健康人类皮肤和血液先天淋巴细胞的深入蛋白质组图谱。

DOI：
10.1016/j.jid.2023.07.011
发表时间：
2024
期刊：
The Journal of investigative dermatology
影响因子：
0
作者：
Teunissen,MarcelBM;PilgaardMøller,LineB;Løvendorf,MarianneB;Skov,Lone;Bonefeld,CharlotteM;Bekkenk,MarcelW;Clark,RachaelA;Mann,Matthias;Dyring-Andersen,Beatrice
通讯作者：
Dyring-Andersen,Beatrice

Induction of IL-1β and antimicrobial peptides as a potential mechanism for topical dithranol.

DOI：
10.1111/exd.14310
发表时间：
2021-06
期刊：
Experimental dermatology
影响因子：
3.6
作者：
Benezeder T;Gehad A;Patra V;Clark R;Wolf P
通讯作者：
Wolf P

CCR8 is a new therapeutic target in cutaneous T-cell lymphomas.

DOI：
10.1182/bloodadvances.2021006512
发表时间：
2022-06-14
期刊：
BLOOD ADVANCES
影响因子：
7.5
作者：
Giustiniani, Jerome;Dobos, Gabor;Moins-Teisserenc, Helene;Eustaquio, Tiago;Battistella, Maxime;Ortonne, Nicolas;Ram-Wolff, Caroline;Bouaziz, Jean-David;Marie-Cardine, Anne;Mourah, Samia;Bagot, Martine;Kupper, Thomas S.;Clark, Rachael A.;Bensussan, Armand;de Masson, Adele
通讯作者：
de Masson, Adele

Resident memory T cells: Runx and hide.

常驻记忆 T 细胞：运行和隐藏。